##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630095.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24326
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.69941626243526	34.0	31.0	34.0	31.0	34.0
2	32.88243032146674	34.0	31.0	34.0	31.0	34.0
3	33.00394639480391	34.0	33.0	34.0	31.0	34.0
4	36.3169448326893	37.0	37.0	37.0	35.0	37.0
5	36.28718243854312	37.0	37.0	37.0	35.0	37.0
6	36.35834086985119	37.0	37.0	37.0	35.0	37.0
7	36.257337827838526	37.0	37.0	37.0	35.0	37.0
8	36.2964318013648	37.0	37.0	37.0	35.0	37.0
9	38.080859985201016	39.0	39.0	39.0	35.0	39.0
10	38.06906190906849	39.0	38.0	39.0	35.0	39.0
11	38.092082545424645	39.0	39.0	39.0	37.0	39.0
12	38.03239332401546	39.0	38.0	39.0	35.0	39.0
13	38.04883663569843	39.0	38.0	39.0	35.0	39.0
14	39.39973690701307	41.0	39.0	41.0	36.0	41.0
15	39.37934720052618	41.0	39.0	41.0	36.0	41.0
16	39.25770780235139	41.0	39.0	41.0	36.0	41.0
17	39.26642275754337	41.0	39.0	41.0	36.0	41.0
18	39.20126613499959	40.0	39.0	41.0	36.0	41.0
19	39.224533421031	41.0	39.0	41.0	36.0	41.0
20	39.40680753103675	41.0	39.0	41.0	36.0	41.0
21	39.38719888185481	41.0	39.0	41.0	36.0	41.0
22	39.35299679355422	41.0	39.0	41.0	36.0	41.0
23	39.28833347036093	41.0	39.0	41.0	35.0	41.0
24	39.19041354928883	41.0	39.0	41.0	35.0	41.0
25	39.1532105566061	41.0	39.0	41.0	35.0	41.0
26	38.89755816821508	41.0	39.0	41.0	35.0	41.0
27	38.740524541642685	40.0	38.0	41.0	35.0	41.0
28	38.63561621310532	40.0	38.0	41.0	35.0	41.0
29	38.427896078270166	40.0	38.0	41.0	35.0	41.0
30	38.199909561785745	40.0	38.0	41.0	34.0	41.0
31	37.89644824467648	40.0	37.0	41.0	34.0	41.0
32	37.70833675902327	40.0	36.0	41.0	33.0	41.0
33	37.538354024500535	40.0	35.0	41.0	33.0	41.0
34	37.36014963413631	40.0	35.0	41.0	33.0	41.0
35	37.11658307983228	40.0	35.0	41.0	33.0	41.0
36	37.00250760503165	40.0	35.0	41.0	32.0	41.0
37	36.80337910055085	40.0	35.0	41.0	31.0	41.0
38	36.566389870920005	40.0	35.0	41.0	31.0	41.0
39	36.32738633560799	40.0	35.0	41.0	30.0	41.0
40	36.11251336019074	40.0	35.0	41.0	28.0	41.0
41	35.80161144454493	39.0	35.0	41.0	26.0	41.0
42	35.52129408862945	39.0	35.0	41.0	23.0	41.0
43	35.040245005344076	39.0	35.0	41.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	8.0
21	8.0
22	26.0
23	31.0
24	52.0
25	79.0
26	126.0
27	160.0
28	231.0
29	260.0
30	330.0
31	395.0
32	496.0
33	620.0
34	778.0
35	1080.0
36	1574.0
37	3101.0
38	4424.0
39	10544.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.675409027378116	19.563430074817067	13.060100304201267	21.701060593603554
2	19.226342185316124	23.89624270328044	34.46107045959056	22.416344651812874
3	20.891227493217134	25.910548384444628	30.794211954287594	22.404012168050645
4	14.235797089533833	16.866726958809505	34.50628956671874	34.391186384937924
5	13.61095124558086	37.94294170846008	33.59779659623448	14.848310449724575
6	31.82602976239415	36.010852585710765	14.749650579626735	17.413467072268354
7	26.111978952561042	31.25051385349009	22.572556112801117	20.064951081147743
8	26.703938173148074	33.39636602811807	20.245827509660447	19.65386828907342
9	24.59508344980679	14.293348680424236	21.23242621063882	39.87914165913015
10	18.26851927978295	27.37400312422922	31.29984378853901	23.05763380744882
11	33.59368576831373	22.695880950423415	21.886047850036995	21.82438543122585
12	20.636356162131055	28.414042588177256	29.363643837868945	21.58595741182274
13	30.683219600427524	20.599358710844363	25.643344569596316	23.074077119131793
14	20.850119214009702	23.296061826851926	28.5414782537203	27.31234070541807
15	24.233330592781385	28.455150867384692	25.18293184247307	22.12858669736085
16	21.705171421524295	27.152018416509083	27.9330757214503	23.209734440516318
17	20.952889912028283	28.685357230946313	27.604209487790843	22.757543369234565
18	21.433856778755242	25.37202992682726	29.055331743813205	24.13878155060429
19	22.843870755570173	26.18186302721368	30.259804324590974	20.714461892625174
20	22.634218531612266	26.46962098166571	30.416015785579216	20.480144701142812
21	21.881937022116254	26.440845186220507	30.46945654854888	21.207761243114362
22	21.553070788456797	27.4767738222478	30.313245087560638	20.65691030173477
23	21.474965057962674	26.642275754336925	30.235139357066515	21.64761983063389
24	21.018663158760173	27.22190249116172	30.350242538847322	21.409191811230784
25	21.080325577571323	26.695716517306582	30.753103675080162	21.47085423004193
26	21.53251664885308	27.06569103017348	30.691441256269016	20.710351064704433
27	21.072103921729838	27.6000986598701	30.47767820439036	20.850119214009702
28	20.554139603716187	27.64942859491902	30.576338074488202	21.220093726876595
29	20.628134506289566	28.25372029926827	30.564005590725973	20.554139603716187
30	20.615802022527337	28.377045136890573	30.51056482775631	20.496588012825782
31	20.850119214009702	28.397599276494283	30.235139357066515	20.5171421524295
32	20.5171421524295	28.187947052536384	30.49001068815259	20.804900106881526
33	20.295157444709364	28.43048589986023	30.461234892707388	20.813121762723014
34	20.044396941544026	28.278385266792732	30.08303872399901	21.594179067664225
35	20.04850776946477	27.653539422839764	31.29984378853901	20.99810901915646
36	20.0896160486722	27.628874455315298	30.592781386171175	21.68872810984132
37	19.896407136397272	27.534325413138205	30.592781386171175	21.97648606429335
38	19.394886130066595	26.975252815917127	31.924689632491983	21.705171421524295
39	19.711419879963824	27.07391268601496	31.562936775466575	21.651730658554634
40	19.415440269670313	27.012250267203815	31.95346542793719	21.618844035188687
41	19.17290142234646	27.061580202252735	31.55060429170435	22.214914083696456
42	18.16985940968511	26.94236619255118	32.4303214667434	22.45745293102031
43	18.161637753843625	26.53950505631834	31.821918934473402	23.47693825536463
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	55.0
1	44.5
2	34.0
3	62.5
4	91.0
5	91.0
6	103.5
7	116.0
8	114.0
9	112.0
10	161.0
11	210.0
12	210.0
13	354.5
14	499.0
15	651.5
16	804.0
17	743.0
18	682.0
19	682.0
20	719.0
21	756.0
22	562.0
23	368.0
24	397.0
25	426.0
26	426.0
27	448.5
28	471.0
29	525.0
30	579.0
31	637.5
32	696.0
33	696.0
34	819.5
35	943.0
36	986.0
37	1029.0
38	1114.5
39	1200.0
40	1200.0
41	1174.0
42	1148.0
43	1200.5
44	1253.0
45	1278.0
46	1303.0
47	1303.0
48	1366.5
49	1430.0
50	1471.0
51	1512.0
52	1526.0
53	1540.0
54	1540.0
55	1469.5
56	1399.0
57	1410.0
58	1421.0
59	1281.5
60	1142.0
61	1142.0
62	1030.0
63	918.0
64	814.5
65	711.0
66	631.5
67	552.0
68	552.0
69	472.0
70	392.0
71	320.5
72	249.0
73	194.5
74	140.0
75	140.0
76	109.5
77	79.0
78	56.0
79	33.0
80	26.5
81	20.0
82	20.0
83	13.5
84	7.0
85	5.0
86	3.0
87	2.5
88	2.0
89	2.0
90	1.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	24326.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.23193291128833
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.3073541759273	75.99687577078024
2	6.781251543438533	11.28833347036093
3	1.1656047809552033	2.9104661678862125
4	0.27164518200227195	0.9043821425635123
5	0.15310910258309873	0.6371783277152018
6	0.044451029782189955	0.22198470772013482
7	0.044451029782189955	0.258982159006824
8	0.0296340198547933	0.1973197401956754
9	0.01975601323652887	0.14798980514675655
>10	0.15310910258309873	2.540491655019321
>50	0.009878006618264434	0.4727452108854723
>100	0.01975601323652887	4.423250842719724
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	355	1.4593439118638494	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	336	1.3812381813697279	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	243	0.9989311847406068	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	142	0.5837375647455397	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58	0.2384280194031078	No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGT	57	0.23431719148236455	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43	0.1767656005919592	No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT	41	0.16854394475047274	RNA PCR Primer, Index 23 (95% over 23bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40	0.1644331168297295	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37	0.1521006330674998	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36	0.14798980514675655	No Hit
GAGTACGGGGGAGGCAGTACAGGCACTAAAAAAAAAAAAAAAA	33	0.13565732138452682	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31	0.12743566554304037	No Hit
AGGCAGTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAA	28	0.11510318178081065	No Hit
GTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27	0.11099235386006741	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.004110827920743238	0.0
19	0.0	0.0	0.0	0.004110827920743238	0.0
20	0.0	0.0	0.0	0.008221655841486476	0.0
21	0.0	0.0	0.0	0.008221655841486476	0.0
22	0.0	0.0	0.0	0.008221655841486476	0.0
23	0.0	0.0	0.0	0.01644331168297295	0.0
24	0.0	0.0	0.0	0.0328866233659459	0.0
25	0.0	0.0	0.0	0.057551590890405326	0.0
26	0.0	0.0	0.0	0.0657732467318918	0.0
27	0.0	0.0	0.0	0.1644331168297295	0.0
28	0.0	0.0	0.0	0.34530954534243197	0.0
29	0.0	0.0	0.0	0.5672942530625668	0.0
30	0.0	0.0	0.0	0.830387239990134	0.0
31	0.0	0.0	0.0	1.2414700320644578	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	140	0.0	27.75	1
GTATCAA	205	0.0	18.951218	2
TACGGGG	50	0.006843397	18.499998	4
ATCAACG	270	0.0	14.388888	4
AACGCAG	275	0.0	14.127272	7
TATCAAC	275	0.0	14.127272	3
TCAACGC	285	0.0	13.631578	5
CAACGCA	290	0.0	13.396551	6
CGCAGAG	295	0.0	13.169492	9
ACGCAGA	310	0.0	12.532257	8
CAGAGTA	310	0.0	12.532257	11
AGAGTAC	315	0.0	12.333333	12
GCAGAGT	320	0.0	12.140625	10
AGTACGG	170	6.353612E-5	10.882353	2
GAGTACG	170	6.353612E-5	10.882353	1
GTACGGG	170	6.353612E-5	10.882353	3
AGTACTT	210	4.92393E-5	9.690475	14
GAGTACT	210	4.92393E-5	9.690475	13
CTTTTTT	260	5.2577954E-5	8.538462	18
GTACTTT	245	2.642739E-4	8.306122	15
>>END_MODULE