FastQCFastQC Report
Fri 10 Feb 2017
ERR1630094.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630094.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55035
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9681.7588807122740073No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9311.6916507676932862No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6731.2228581811574453No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4300.7813209775597346No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1730.3143454165531026No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1480.26891977832288544No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1290.2343962932679204No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1120.20350685927137277No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1100.1998728082129554No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTA1090.19805578268374668No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.16716634868719904No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA850.15444716998273827No Hit
CCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAA750.1362769146906514No Hit
GCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGA710.12900881257381666No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC700.12719178704460798No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACA670.12174071045698191No Hit
GCATAGTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGT610.11083855728172981No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA570.10357045516489506No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG560.10175342963568638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATCT308.168887E-637.04
AGAAATC308.168887E-637.03
CAGAAAT308.168887E-637.02
CTTGAAA251.21840814E-437.027
TCTTCTG308.168887E-637.020
CGTCTTC308.168887E-637.018
GTATGCC352.347519E-531.71428512
AAATCTC352.347519E-531.7142855
TATGCCG352.347519E-531.71428513
CCGTCTT352.347519E-531.71428517
CGTATGC352.347519E-531.71428511
TCGTATG352.347519E-531.71428510
GCCGTCT352.347519E-531.71428516
CTCGTAT352.347519E-531.7142859
AGGGTTG250.005451522329.65
AATCTCG405.8394897E-527.756
TCTCGTA405.8394897E-527.758
GTCTTCT405.8394897E-527.7519
TATACAC358.762034E-426.4285737
GGTATCA3100.025.6612911
Produced by FastQC (version 0.11.5)