##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630094.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	55035
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.65494685200327	33.0	31.0	34.0	31.0	34.0
2	32.84686108839829	34.0	31.0	34.0	31.0	34.0
3	32.94846915599164	34.0	31.0	34.0	31.0	34.0
4	36.24335422912692	37.0	37.0	37.0	35.0	37.0
5	36.25414736077042	37.0	37.0	37.0	35.0	37.0
6	36.3690015444717	37.0	37.0	37.0	35.0	37.0
7	36.29059689288634	37.0	37.0	37.0	35.0	37.0
8	36.34214590715	37.0	37.0	37.0	35.0	37.0
9	38.05407467974925	39.0	38.0	39.0	35.0	39.0
10	38.057581539020624	39.0	38.0	39.0	37.0	39.0
11	38.11180158081221	39.0	39.0	39.0	37.0	39.0
12	38.0275461070228	39.0	38.0	39.0	35.0	39.0
13	38.07413464159171	39.0	38.0	39.0	35.0	39.0
14	39.36305987099119	41.0	39.0	41.0	36.0	41.0
15	39.34921413645862	41.0	39.0	41.0	36.0	41.0
16	39.24090124466249	41.0	39.0	41.0	36.0	41.0
17	39.27887707822295	40.0	39.0	41.0	36.0	41.0
18	39.247769601162894	40.0	39.0	41.0	36.0	41.0
19	39.29219587535205	41.0	39.0	41.0	36.0	41.0
20	39.428327428000365	41.0	39.0	41.0	36.0	41.0
21	39.39411283728536	41.0	39.0	41.0	36.0	41.0
22	39.37439811029345	41.0	39.0	41.0	36.0	41.0
23	39.292613791223765	41.0	39.0	41.0	35.0	41.0
24	39.22634687017353	41.0	39.0	41.0	35.0	41.0
25	39.16718451894249	41.0	39.0	41.0	35.0	41.0
26	38.89379485781775	41.0	38.0	41.0	35.0	41.0
27	38.75417461615336	40.0	38.0	41.0	35.0	41.0
28	38.63917507040974	40.0	38.0	41.0	35.0	41.0
29	38.495393840283455	40.0	38.0	41.0	35.0	41.0
30	38.19831016625783	40.0	37.0	41.0	35.0	41.0
31	37.959716544017446	40.0	37.0	41.0	34.0	41.0
32	37.777650585990735	40.0	36.0	41.0	34.0	41.0
33	37.603179794676116	40.0	35.0	41.0	33.0	41.0
34	37.42879985463796	40.0	35.0	41.0	33.0	41.0
35	37.18041246479513	40.0	35.0	41.0	33.0	41.0
36	36.991787044607975	40.0	35.0	41.0	33.0	41.0
37	36.77941310075406	40.0	35.0	41.0	31.0	41.0
38	36.54367220859453	40.0	35.0	41.0	31.0	41.0
39	36.32780957572454	40.0	35.0	41.0	30.0	41.0
40	36.12646497683293	40.0	35.0	41.0	29.0	41.0
41	35.83252475697284	39.0	35.0	41.0	26.0	41.0
42	35.537294448987005	39.0	35.0	41.0	24.0	41.0
43	35.07971290996638	39.0	35.0	41.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	0.0
16	0.0
17	4.0
18	3.0
19	2.0
20	8.0
21	25.0
22	43.0
23	67.0
24	101.0
25	160.0
26	230.0
27	340.0
28	479.0
29	632.0
30	722.0
31	931.0
32	1089.0
33	1402.0
34	1790.0
35	2372.0
36	3666.0
37	7427.0
38	10381.0
39	23159.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.085218497319886	19.598437358044883	13.044426274189153	21.27191787044608
2	19.851003906604888	23.14890524211865	33.233396929226856	23.766693922049605
3	20.063595893522304	25.311165621876984	32.60288907059144	22.02234941400927
4	13.091668937948578	17.890433360588716	35.84446261469974	33.17343508676297
5	11.861542654674299	39.62750976651222	35.2939038793495	13.217043699463977
6	30.251658035795405	37.559734714272736	14.663396020714092	17.52521122921777
7	25.79086036158808	33.0753157081857	22.596529481239212	18.53729444898701
8	28.065776324157355	32.95175797219951	19.187789588443717	19.79467611519942
9	24.99136912873626	13.778504587989463	21.6734805124012	39.55664577087308
10	18.624511674389023	27.97492504769692	31.343690378849825	22.05687289906423
11	33.18252021440901	23.04170073589534	21.942400290724084	21.833378758971563
12	20.71954210956664	30.971200145362044	27.9912782774598	20.31797946761152
13	30.822204051966928	21.64804215499228	25.505587353502317	22.024166439538476
14	19.273189788316525	24.277278095757246	30.084491687108205	26.365040428818027
15	24.457163623148904	28.163895702734624	26.78295630053602	20.595984373580446
16	20.95757245389298	26.664849641137454	28.46007086399564	23.917507040973927
17	20.62142273098937	29.143272462978103	28.483692195875353	21.751612610157174
18	21.002998092123196	26.506768420096304	29.6029799218679	22.8872535659126
19	23.081675297537927	25.58371945125829	32.117743254292726	19.216861996911057
20	21.262832742800036	26.174252748251114	32.53565912601072	20.02725538293813
21	20.919414917779594	26.52130462432997	31.956027982193152	20.603252475697285
22	20.018170255292087	27.377123648587265	32.23584991369129	20.36885618242936
23	20.367039156900155	26.835650040883074	32.553829381302805	20.243481420913962
24	20.183519578450078	27.756881984191878	31.492686472244934	20.56691196511311
25	19.135095848096668	27.67148178431907	32.62832742800036	20.5650949395839
26	19.229581175615518	28.24929590260743	32.67920414281821	19.841918778958846
27	19.37676024348142	28.36376851094758	31.4436267829563	20.8158444626147
28	19.718361042972653	28.038520941219225	32.922685563732166	19.320432452075952
29	20.824929590260744	28.40192604706096	31.354592532025077	19.41855183065322
30	19.353138911601707	28.10029980921232	33.44780594167348	19.09875533751249
31	19.00790406105206	28.358317434359954	32.32125011356409	20.312528391023893
32	17.58153902062324	28.212955392023257	32.87362587444353	21.331879712909966
33	18.453711274643407	28.798037612428455	32.971745253020806	19.77650585990733
34	19.151449077859546	28.178431906968292	32.688289270464246	19.981829744707913
35	18.735350231670754	29.524847824111927	32.04142818206596	19.69837376215136
36	20.824929590260744	27.93676751158354	30.856727537021893	20.381575361133823
37	18.642681929681114	27.85500136276915	31.25283910238939	22.249477605160354
38	19.13872989915508	26.915599164168256	33.25883528663578	20.686835650040884
39	18.008540019987283	27.179067865903516	33.22612882711002	21.586263286999184
40	17.45798128463705	27.386208776233307	34.34541655310257	20.810393386027073
41	17.839556645770873	26.968292904515305	34.12555646406832	21.066593985645497
42	17.66330516943763	27.393476878350143	33.27518851639866	21.668029435813573
43	17.701462705551013	26.586717543381482	33.68947033705824	22.02234941400927
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	195.0
1	154.5
2	114.0
3	196.5
4	279.0
5	279.0
6	310.5
7	342.0
8	312.5
9	283.0
10	396.0
11	509.0
12	509.0
13	857.0
14	1205.0
15	1596.0
16	1987.0
17	1793.5
18	1600.0
19	1600.0
20	1736.0
21	1872.0
22	1276.5
23	681.0
24	664.5
25	648.0
26	648.0
27	720.5
28	793.0
29	921.0
30	1049.0
31	1275.0
32	1501.0
33	1501.0
34	1827.5
35	2154.0
36	2521.5
37	2889.0
38	2993.0
39	3097.0
40	3097.0
41	3338.5
42	3580.0
43	3920.0
44	4260.0
45	4117.5
46	3975.0
47	3975.0
48	3942.0
49	3909.0
50	3657.0
51	3405.0
52	3083.0
53	2761.0
54	2761.0
55	2680.0
56	2599.0
57	2370.0
58	2141.0
59	1990.5
60	1840.0
61	1840.0
62	1672.5
63	1505.0
64	1365.0
65	1225.0
66	1074.5
67	924.0
68	924.0
69	781.0
70	638.0
71	533.0
72	428.0
73	349.0
74	270.0
75	270.0
76	220.0
77	170.0
78	125.5
79	81.0
80	69.5
81	58.0
82	58.0
83	44.5
84	31.0
85	25.5
86	20.0
87	14.5
88	9.0
89	9.0
90	7.5
91	6.0
92	3.5
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	55035.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.952121377305346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.97376658220301	51.18379213227946
2	9.193620509763004	11.207413464159172
3	2.5100611119391862	4.589806486781139
4	1.070204203308988	2.609248659943672
5	0.6051572514532717	1.8442809121468156
6	0.45610374124310626	1.6680294358135732
7	0.33984200327917724	1.449986372308531
8	0.26233417796989117	1.2791859725629144
9	0.19078849306901177	1.0466067048242027
>10	1.3325383812788791	14.806941037521575
>50	0.03577284245043971	1.4409012446624874
>100	0.020867491429423162	2.200417915871718
>500	0.008943210612609928	4.673389661124739
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	968	1.7588807122740073	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	931	1.6916507676932862	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	673	1.2228581811574453	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	430	0.7813209775597346	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	173	0.3143454165531026	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	148	0.26891977832288544	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	129	0.2343962932679204	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	112	0.20350685927137277	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	110	0.1998728082129554	No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTA	109	0.19805578268374668	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	92	0.16716634868719904	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	85	0.15444716998273827	No Hit
CCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAA	75	0.1362769146906514	No Hit
GCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGA	71	0.12900881257381666	No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC	70	0.12719178704460798	No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACA	67	0.12174071045698191	No Hit
GCATAGTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGT	61	0.11083855728172981	No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA	57	0.10357045516489506	No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG	56	0.10175342963568638	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0018170255292086854	0.0
14	0.0	0.0	0.0	0.0018170255292086854	0.0
15	0.0	0.0	0.0	0.0018170255292086854	0.0
16	0.0	0.0	0.0	0.0018170255292086854	0.0
17	0.0	0.0	0.0	0.0018170255292086854	0.0
18	0.0	0.0	0.0	0.0018170255292086854	0.0
19	0.0	0.0	0.0	0.0018170255292086854	0.0
20	0.0	0.0	0.0	0.0018170255292086854	0.0
21	0.0	0.0	0.0	0.003634051058417371	0.0
22	0.0	0.0	0.0	0.009085127646043427	0.0
23	0.0	0.0	0.0	0.010902153175252113	0.0
24	0.0	0.0	0.0	0.016353229762878167	0.0
25	0.0	0.0	0.0	0.029072408467338967	0.0
26	0.0	0.0	0.0	0.03815753611338239	0.0
27	0.0	0.0	0.0	0.1235577359861906	0.0
28	0.0	0.0	0.0	0.2834559825565549	0.0
29	0.0	0.0	0.0	0.5469246842918143	0.0
30	0.0	0.0	0.0	0.8212955392023258	0.0
31	0.0	0.0	0.0	1.275551921504497	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAAATCT	30	8.168887E-6	37.0	4
AGAAATC	30	8.168887E-6	37.0	3
CAGAAAT	30	8.168887E-6	37.0	2
CTTGAAA	25	1.21840814E-4	37.0	27
TCTTCTG	30	8.168887E-6	37.0	20
CGTCTTC	30	8.168887E-6	37.0	18
GTATGCC	35	2.347519E-5	31.714285	12
AAATCTC	35	2.347519E-5	31.714285	5
TATGCCG	35	2.347519E-5	31.714285	13
CCGTCTT	35	2.347519E-5	31.714285	17
CGTATGC	35	2.347519E-5	31.714285	11
TCGTATG	35	2.347519E-5	31.714285	10
GCCGTCT	35	2.347519E-5	31.714285	16
CTCGTAT	35	2.347519E-5	31.714285	9
AGGGTTG	25	0.0054515223	29.6	5
AATCTCG	40	5.8394897E-5	27.75	6
TCTCGTA	40	5.8394897E-5	27.75	8
GTCTTCT	40	5.8394897E-5	27.75	19
TATACAC	35	8.762034E-4	26.42857	37
GGTATCA	310	0.0	25.661291	1
>>END_MODULE