FastQCFastQC Report
Fri 10 Feb 2017
ERR1630093.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630093.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2819032
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT100910.35795975356079673No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT82140.2913766143839446No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT66760.23681887967217113No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47530.16860397469769764No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG28200.10003433802808906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20150.029.1960321
CGTGCGA2150.018.93023310
CGGTAAT650.001580703717.0769231
GTATCAA34950.016.7796862
CGAGAAT2400.016.187514
CCGTACG957.065492E-515.57894813
CGAATTA2750.015.47272815
CCGTGCG3250.014.89
TAGGACA7300.014.4452074
GTGCGAG2700.014.38888911
GTACGAT1051.6569783E-414.0952386
CGTACGC950.001245858813.63157914
CGATAGT1409.569792E-613.21428528
TGCGAGA3100.013.12903212
TACGCCG850.00940916413.05882335
TCTATAC3400.013.0588233
TTAACGG1000.001833628612.95000135
TAACGGC1153.5800386E-412.86956536
TATCAAC46150.012.8678233
TTAGGAC7700.012.7337663
Produced by FastQC (version 0.11.5)