##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630093.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2819032
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83107818570346	34.0	31.0	34.0	31.0	34.0
2	33.0560359016854	34.0	33.0	34.0	31.0	34.0
3	33.1831213693211	34.0	33.0	34.0	31.0	34.0
4	36.4766813572886	37.0	37.0	37.0	35.0	37.0
5	36.42842755953107	37.0	37.0	37.0	35.0	37.0
6	36.47446783151096	37.0	37.0	37.0	35.0	37.0
7	36.40459100854478	37.0	37.0	37.0	35.0	37.0
8	36.415006285845635	37.0	37.0	37.0	35.0	37.0
9	38.18444664693413	39.0	39.0	39.0	37.0	39.0
10	38.16432910303963	39.0	39.0	39.0	37.0	39.0
11	38.199311323887066	39.0	39.0	39.0	37.0	39.0
12	38.12762146722705	39.0	39.0	39.0	37.0	39.0
13	38.148089131304644	39.0	39.0	39.0	37.0	39.0
14	39.601857658941086	41.0	40.0	41.0	37.0	41.0
15	39.588478243595674	41.0	40.0	41.0	37.0	41.0
16	39.513187505498344	41.0	39.0	41.0	37.0	41.0
17	39.43347574628454	41.0	39.0	41.0	37.0	41.0
18	39.32236633000264	41.0	39.0	41.0	36.0	41.0
19	39.35377711214346	41.0	39.0	41.0	36.0	41.0
20	39.57531273146243	41.0	39.0	41.0	37.0	41.0
21	39.5850203190315	41.0	40.0	41.0	37.0	41.0
22	39.609952636224065	41.0	40.0	41.0	37.0	41.0
23	39.549016825633764	41.0	40.0	41.0	37.0	41.0
24	39.515318024059326	41.0	40.0	41.0	37.0	41.0
25	39.48779722968735	41.0	40.0	41.0	37.0	41.0
26	39.35363202688015	41.0	39.0	41.0	36.0	41.0
27	39.268963601690224	41.0	39.0	41.0	36.0	41.0
28	39.23357166573491	41.0	39.0	41.0	36.0	41.0
29	39.13076651843612	41.0	39.0	41.0	36.0	41.0
30	39.04428399535727	40.0	39.0	41.0	35.0	41.0
31	38.90880522108298	40.0	39.0	41.0	35.0	41.0
32	38.824425192761204	40.0	39.0	41.0	35.0	41.0
33	38.74873431731176	40.0	38.0	41.0	35.0	41.0
34	38.67084552427926	40.0	38.0	41.0	35.0	41.0
35	38.55621965270348	40.0	38.0	41.0	35.0	41.0
36	38.538468169215534	40.0	38.0	41.0	35.0	41.0
37	38.48357876036881	40.0	38.0	41.0	35.0	41.0
38	38.3876947831738	40.0	38.0	41.0	35.0	41.0
39	38.32765679850388	40.0	38.0	41.0	34.0	41.0
40	38.271762789496535	40.0	38.0	41.0	34.0	41.0
41	38.16516662457184	40.0	38.0	41.0	34.0	41.0
42	38.07402079862875	40.0	38.0	41.0	34.0	41.0
43	37.77959881264207	40.0	38.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	2.0
14	2.0
15	4.0
16	15.0
17	34.0
18	59.0
19	120.0
20	257.0
21	495.0
22	930.0
23	1623.0
24	2736.0
25	4290.0
26	6238.0
27	8686.0
28	11809.0
29	15954.0
30	20953.0
31	26711.0
32	34674.0
33	45985.0
34	62792.0
35	88538.0
36	136194.0
37	245544.0
38	532027.0
39	1572359.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.43345517184623	19.50850504712256	12.174533669713576	24.883506111317644
2	18.60259124408662	21.45697530216046	35.704419105565314	24.236014348187602
3	19.073391149869884	23.362629441595555	31.04672100210285	26.517258406431708
4	13.92790149242719	16.106344305421153	36.137972183359395	33.82778201879227
5	14.004133333711714	36.77311928349874	35.20105483016865	14.021692552620898
6	33.58464891494669	36.227861194906616	15.64494478955897	14.54254510058772
7	28.14047517020027	30.759743060738582	22.365549592909908	18.73423217615125
8	25.647172504604416	34.806273926652835	20.61548077496105	18.931072793781695
9	25.158458648216836	14.651483204163698	20.76496471129097	39.4250934363285
10	16.100668598298988	27.363435392006902	34.940185141566324	21.59571086812778
11	34.086594263562816	22.763487608512424	22.4075498256139	20.742368302310865
12	19.957630846333068	25.909390173648262	30.8420053408404	23.290973639178272
13	29.413536277700995	20.807922719571824	25.732237165097803	24.04630383762937
14	21.628700915775344	21.942673939139393	26.42375113159411	30.004874013491158
15	23.584088438868378	29.07678947950928	23.893095218500534	23.446026863121812
16	23.7645759253531	27.41923468765165	25.246361162271302	23.56982822472395
17	22.48310767667767	27.512103445438008	26.72516665295037	23.27962222493395
18	22.135257776428222	26.372137669951957	27.92501113857523	23.5675934150446
19	23.962267899051874	26.357132519247738	28.07786502600893	21.60273455569146
20	23.75322451110878	26.302042686993264	27.859740506670377	22.084992295227583
21	22.43113948334038	26.86255423847619	27.310580369431776	23.395725908751658
22	23.30118991199816	26.06508191464304	27.526221766904385	23.107506406454416
23	22.31372329225067	26.54031596661549	27.60685228120858	23.539108459925252
24	23.114991245221763	26.698881034340864	27.227502206431144	22.958625514006226
25	23.347340505535232	26.135496156127353	27.644985938435607	22.872177399901812
26	23.413604386186464	26.529603069422414	26.93449382624958	23.122298718141547
27	23.069550115074964	26.58533141872813	26.88046818908051	23.4646502771164
28	22.73003640966119	26.610269056896126	27.675386444708682	22.984308088734004
29	22.99420510302827	26.867910687072726	27.842784331642918	22.29509987825608
30	22.971927952573793	26.83226015171165	26.991392790149245	23.204419105565314
31	22.797293539058796	26.174587588931235	27.798017191716873	23.230101680293096
32	22.348806256899532	26.731232564937184	27.59546539379475	23.324495784368533
33	22.666433016723474	26.26596647359803	27.675989488590407	23.391611021088092
34	23.100979343263926	25.84848274159357	27.910892817108852	23.13964509803365
35	23.08317181216815	26.038938188711587	27.473012012634122	23.40487798648614
36	22.646993719830068	25.841707366216486	27.509975055267198	24.001323858686245
37	22.50428515887723	24.956509894176442	28.832875965934406	23.70632898101192
38	22.285734961504517	25.14888089244819	28.788428084533983	23.776956061513314
39	21.73831300957208	25.464379262101318	29.675505634558245	23.121802093768355
40	22.265408835373275	24.4631845257521	29.648758864745062	23.62264777412956
41	21.16272536104592	25.152073477704402	29.849111326157345	23.83608983509233
42	20.979258128322062	25.2263897678352	29.59100854477707	24.203343559065665
43	21.19752454033867	24.806032709100144	29.674193127286248	24.32224962327494
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2563.0
1	2025.0
2	1487.0
3	2834.0
4	4181.0
5	4181.0
6	4713.0
7	5245.0
8	4650.0
9	4055.0
10	5452.5
11	6850.0
12	6850.0
13	10370.5
14	13891.0
15	18146.0
16	22401.0
17	23388.0
18	24375.0
19	24375.0
20	30272.5
21	36170.0
22	37597.0
23	39024.0
24	44960.0
25	50896.0
26	50896.0
27	56523.5
28	62151.0
29	73868.0
30	85585.0
31	93015.5
32	100446.0
33	100446.0
34	113728.5
35	127011.0
36	137173.5
37	147336.0
38	151962.0
39	156588.0
40	156588.0
41	167638.0
42	178688.0
43	179102.0
44	179516.0
45	180089.0
46	180662.0
47	180662.0
48	181482.5
49	182303.0
50	187016.0
51	191729.0
52	196550.0
53	201371.0
54	201371.0
55	189603.5
56	177836.0
57	159901.0
58	141966.0
59	133603.0
60	125240.0
61	125240.0
62	116718.0
63	108196.0
64	90804.0
65	73412.0
66	65273.5
67	57135.0
68	57135.0
69	50150.0
70	43165.0
71	41337.0
72	39509.0
73	34084.0
74	28659.0
75	28659.0
76	18738.0
77	8817.0
78	7059.5
79	5302.0
80	4035.5
81	2769.0
82	2769.0
83	2056.5
84	1344.0
85	985.0
86	626.0
87	464.5
88	303.0
89	303.0
90	222.0
91	141.0
92	96.0
93	51.0
94	37.0
95	23.0
96	23.0
97	15.5
98	8.0
99	7.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2819032.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.99328243819946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.44877952530133	39.71905274178628
2	11.701322173590148	11.699750086493166
3	3.572278000107312	5.357697090213934
4	1.6185073396105851	3.2365797822980316
5	0.8690013031552967	2.1722113793903066
6	0.5439750147620704	1.6317057931394348
7	0.3637688614012752	1.273019960117933
8	0.2588004230915507	1.0350626117393131
9	0.20322392850808912	0.9143848130494819
>10	1.1816061216095817	11.184940139702384
>50	0.12431753973786384	4.346315042165527
>100	0.0995886620499519	9.78089611439771
>500	0.010818826900242554	3.7814958351203893
>1k	0.0037973365941175335	2.972091245014449
>5k	1.4329572053273712E-4	0.5333466542725538
>10k+	7.164786026636856E-5	0.36145071109901544
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10091	0.35795975356079673	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8214	0.2913766143839446	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6676	0.23681887967217113	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4753	0.16860397469769764	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	2820	0.10003433802808906	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.5473169513506765E-5	0.0
3	0.0	0.0	0.0	3.5473169513506765E-5	0.0
4	0.0	0.0	0.0	3.5473169513506765E-5	0.0
5	0.0	0.0	0.0	3.5473169513506765E-5	0.0
6	0.0	0.0	0.0	3.5473169513506765E-5	0.0
7	3.5473169513506765E-5	0.0	0.0	7.094633902701353E-5	0.0
8	3.5473169513506765E-5	0.0	0.0	7.094633902701353E-5	0.0
9	7.094633902701353E-5	0.0	0.0	7.094633902701353E-5	0.0
10	1.4189267805402706E-4	0.0	0.0	1.773658475675338E-4	0.0
11	1.773658475675338E-4	0.0	0.0	2.128390170810406E-4	0.0
12	1.773658475675338E-4	0.0	0.0	3.1925852562156087E-4	0.0
13	1.773658475675338E-4	0.0	0.0	3.1925852562156087E-4	0.0
14	1.773658475675338E-4	0.0	0.0	3.1925852562156087E-4	0.0
15	1.773658475675338E-4	0.0	0.0	3.1925852562156087E-4	0.0
16	1.773658475675338E-4	0.0	0.0	3.547316951350676E-4	7.094633902701353E-5
17	2.4831218659454737E-4	0.0	0.0	4.256780341620812E-4	7.094633902701353E-5
18	2.4831218659454737E-4	0.0	0.0	4.6115120367558793E-4	7.094633902701353E-5
19	2.837853561080541E-4	0.0	0.0	5.320975427026014E-4	7.094633902701353E-5
20	2.837853561080541E-4	0.0	0.0	6.739902207566285E-4	7.094633902701353E-5
21	2.837853561080541E-4	0.0	0.0	8.158828988106555E-4	1.064195085405203E-4
22	3.1925852562156087E-4	0.0	0.0	0.0012770341024862435	1.064195085405203E-4
23	3.1925852562156087E-4	0.0	0.0	0.002802380391567034	1.064195085405203E-4
24	3.1925852562156087E-4	0.0	0.0	0.006633482699025765	1.064195085405203E-4
25	3.1925852562156087E-4	0.0	0.0	0.012663921516321914	1.064195085405203E-4
26	3.1925852562156087E-4	0.0	0.0	0.022986613844752382	1.064195085405203E-4
27	3.902048646485744E-4	0.0	0.0	0.07775718757360682	1.064195085405203E-4
28	4.256780341620812E-4	7.094633902701353E-5	0.0	0.18328986687628945	1.064195085405203E-4
29	4.256780341620812E-4	1.064195085405203E-4	0.0	0.2960590727597274	1.064195085405203E-4
30	4.256780341620812E-4	1.064195085405203E-4	0.0	0.4179094100386232	1.064195085405203E-4
31	4.256780341620812E-4	1.064195085405203E-4	0.0	0.6966221029062458	1.064195085405203E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2015	0.0	29.196032	1
CGTGCGA	215	0.0	18.930233	10
CGGTAAT	65	0.0015807037	17.076923	1
GTATCAA	3495	0.0	16.779686	2
CGAGAAT	240	0.0	16.1875	14
CCGTACG	95	7.065492E-5	15.578948	13
CGAATTA	275	0.0	15.472728	15
CCGTGCG	325	0.0	14.8	9
TAGGACA	730	0.0	14.445207	4
GTGCGAG	270	0.0	14.388889	11
GTACGAT	105	1.6569783E-4	14.095238	6
CGTACGC	95	0.0012458588	13.631579	14
CGATAGT	140	9.569792E-6	13.214285	28
TGCGAGA	310	0.0	13.129032	12
TACGCCG	85	0.009409164	13.058823	35
TCTATAC	340	0.0	13.058823	3
TTAACGG	100	0.0018336286	12.950001	35
TAACGGC	115	3.5800386E-4	12.869565	36
TATCAAC	4615	0.0	12.867823	3
TTAGGAC	770	0.0	12.733766	3
>>END_MODULE