FastQCFastQC Report
Fri 10 Feb 2017
ERR1630092.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630092.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1130914
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT64320.5687435118850771No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53560.47359923035703866No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42270.3737684739953701No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32390.28640550917222707No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24250.21442832965194525No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20630.18241882229771672No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17210.15217779601278258No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13620.12043356081894822No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC12180.10770049712002859No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA11580.10239505391214539No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA11460.10133396527056877No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT11410.10089184500324516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13850.031.5234661
TAGTCGG450.003825771220.55555530
GTATCAA22850.019.2691482
TTAGTCG609.236692E-418.55
GGACCGC701.2191752E-418.56
CGCCGTA500.00703481418.525
AACTCCG700.002592458915.8571437
ACCTAGG4300.014.6279071
AACCACG908.2738703E-414.3888887
TCTGTCG908.2738703E-414.3888888
CTAGCAC1551.209828E-714.3225813
GTATTAG2600.014.2307691
CTACACT1458.90881E-714.0344834
ATAGGAC800.00629853713.8750013
GCTCTAT950.001245051913.6315791
TTAGTAC950.001245051913.6315793
GTACTAT1501.3053268E-613.5666671
TCTATAC1501.3053268E-613.5666673
ATCAACG32750.013.5007644
TATCAAC33350.013.479763
Produced by FastQC (version 0.11.5)