##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630092.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1130914
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83843156950926	34.0	31.0	34.0	31.0	34.0
2	33.05800529483232	34.0	33.0	34.0	31.0	34.0
3	33.18426246381246	34.0	33.0	34.0	31.0	34.0
4	36.463700157571665	37.0	37.0	37.0	35.0	37.0
5	36.423096716461195	37.0	37.0	37.0	35.0	37.0
6	36.47823265075859	37.0	37.0	37.0	35.0	37.0
7	36.40586552116253	37.0	37.0	37.0	35.0	37.0
8	36.41848186511088	37.0	37.0	37.0	35.0	37.0
9	38.1865809424943	39.0	39.0	39.0	37.0	39.0
10	38.18036207881413	39.0	39.0	39.0	37.0	39.0
11	38.20476534908932	39.0	39.0	39.0	37.0	39.0
12	38.14744976187402	39.0	39.0	39.0	37.0	39.0
13	38.167860686135285	39.0	39.0	39.0	37.0	39.0
14	39.60387085136447	41.0	40.0	41.0	37.0	41.0
15	39.60031708865572	41.0	40.0	41.0	37.0	41.0
16	39.50847102432192	41.0	39.0	41.0	37.0	41.0
17	39.45606208783338	41.0	39.0	41.0	37.0	41.0
18	39.36371289063536	41.0	39.0	41.0	36.0	41.0
19	39.37618068217389	41.0	39.0	41.0	36.0	41.0
20	39.59752465704731	41.0	39.0	41.0	37.0	41.0
21	39.59518141963049	41.0	40.0	41.0	37.0	41.0
22	39.605508464834635	41.0	40.0	41.0	37.0	41.0
23	39.53434478660623	41.0	40.0	41.0	37.0	41.0
24	39.502257466084956	41.0	40.0	41.0	37.0	41.0
25	39.4843197625991	41.0	40.0	41.0	36.0	41.0
26	39.329915448920076	41.0	39.0	41.0	36.0	41.0
27	39.241491395455355	41.0	39.0	41.0	36.0	41.0
28	39.18974210240567	41.0	39.0	41.0	35.0	41.0
29	39.08890684879664	41.0	39.0	41.0	35.0	41.0
30	38.997188115099824	40.0	39.0	41.0	35.0	41.0
31	38.848242218241175	40.0	39.0	41.0	35.0	41.0
32	38.76611042042101	40.0	38.0	41.0	35.0	41.0
33	38.67910203605226	40.0	38.0	41.0	35.0	41.0
34	38.604727680442544	40.0	38.0	41.0	35.0	41.0
35	38.477149456103646	40.0	38.0	41.0	35.0	41.0
36	38.452736459182574	40.0	38.0	41.0	35.0	41.0
37	38.40304833081914	40.0	38.0	41.0	35.0	41.0
38	38.30567664738433	40.0	38.0	41.0	35.0	41.0
39	38.2452874400706	40.0	38.0	41.0	34.0	41.0
40	38.17311926459483	40.0	38.0	41.0	34.0	41.0
41	38.073441481845656	40.0	38.0	41.0	34.0	41.0
42	37.97531023579158	40.0	38.0	41.0	34.0	41.0
43	37.68577716784831	40.0	38.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	3.0
14	2.0
15	2.0
16	5.0
17	11.0
18	25.0
19	50.0
20	107.0
21	193.0
22	383.0
23	682.0
24	1101.0
25	1660.0
26	2451.0
27	3524.0
28	4901.0
29	6606.0
30	8483.0
31	11191.0
32	14417.0
33	18796.0
34	25989.0
35	36991.0
36	58994.0
37	101953.0
38	202550.0
39	629841.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.328139893926505	19.60414319744914	12.776214637010419	24.291502271613933
2	18.804878178181543	21.82951135099574	34.81228457689975	24.55332589392297
3	19.42013274218906	23.497719543661145	31.107493584834923	25.97465412931487
4	14.04890203852813	16.116963800960992	36.23414335661244	33.599990803898436
5	13.901057021135117	36.81968743865581	35.24874570480161	14.030509835407468
6	33.25381063458406	36.12582389111816	15.439281855207382	15.1810836190904
7	27.58467929480049	31.181150821371034	22.466695080262514	18.767474803565964
8	26.422610384167143	33.73209633977473	20.705022663084904	19.14027061297322
9	25.192012832098637	14.907588021724022	21.310373733104374	38.590025413072965
10	16.748134694592164	27.51049151394359	34.41313839956	21.328235391904247
11	33.23488788714261	23.02677303490805	22.716050911033022	21.02228816691632
12	21.11805141681861	26.37627618015163	30.55696542796358	21.948706975066184
13	29.987868219864637	21.122295771384916	25.289102442802903	23.600733565947543
14	21.27376617496998	22.38003950786709	27.253973334842435	29.092220982320494
15	24.005273610548635	29.05278385447523	23.973794647515195	22.968147887460937
16	22.54123655733327	27.78646298480698	26.01081956718194	23.661480890677804
17	22.170739773316097	27.55868262308186	27.349471312584335	22.921106291017708
18	22.16092470338151	26.309869715999625	28.43195857509943	23.09724700551943
19	23.583932995789247	26.013030168518565	29.07869210214039	21.3243447335518
20	23.815427167759886	26.20800520640827	27.95075487614443	22.02581274968742
21	22.730906152015095	26.260352246059384	28.414185340353026	22.5945562615725
22	22.865399137334936	26.557987610021627	28.507649564865233	22.068963687778204
23	22.82675782597085	26.376364604205094	27.881165146067694	22.91571242375636
24	22.43008752212812	26.615286396666765	28.523654318542345	22.430971762662764
25	22.844265788556868	26.808050833219855	27.98470971267488	22.362973665548395
26	22.635054478059338	26.624305650120167	28.17376033898245	22.56687953283804
27	22.3203532717784	26.361597787276487	28.427891068640054	22.890157872305057
28	22.39542529316995	26.583542161472934	28.147056274836107	22.873976270521013
29	22.38976615374821	26.963677167317762	28.553718496720354	22.09283818221368
30	22.90165299925547	26.691773202913748	27.804236219553385	22.602337578277393
31	22.22980704103053	26.98834747823442	28.589883934587423	22.191961546147628
32	21.92014600579708	26.678774867054432	28.631354815662373	22.769724311486108
33	21.550091342047228	26.754996401141025	28.901313450890164	22.793598805921583
34	22.684571947999583	25.51537959561912	28.737021559552716	23.063026896828585
35	21.98681774210948	25.793384819712195	28.85842778495978	23.361369653218546
36	21.837292667700638	26.099508892807055	29.143860629543887	22.919337809948416
37	21.814744534067135	25.097399094891387	29.706414457686435	23.38144191335504
38	21.84569295277979	25.350115039693556	29.892989210496996	22.91120279702966
39	21.325582670300307	25.019939624056292	30.478886988754226	23.17559071688917
40	20.70741011252845	24.63016639638381	31.131721775484255	23.530701715603485
41	20.275458611353294	24.733976235151392	31.164527099319667	23.82603805417565
42	19.97145671554159	25.018171142987	31.403095195567477	23.607276945903934
43	20.19402005811229	24.990759686412936	31.282131090427747	23.533089165047034
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4154.0
1	2884.0
2	1614.0
3	2474.0
4	3334.0
5	3334.0
6	3464.5
7	3595.0
8	2916.0
9	2237.0
10	3203.0
11	4169.0
12	4169.0
13	6545.5
14	8922.0
15	10862.5
16	12803.0
17	13045.5
18	13288.0
19	13288.0
20	16117.5
21	18947.0
22	18013.0
23	17079.0
24	19901.0
25	22723.0
26	22723.0
27	25715.5
28	28708.0
29	31943.5
30	35179.0
31	38864.5
32	42550.0
33	42550.0
34	46043.5
35	49537.0
36	52668.5
37	55800.0
38	57766.0
39	59732.0
40	59732.0
41	61634.0
42	63536.0
43	63668.5
44	63801.0
45	64791.0
46	65781.0
47	65781.0
48	67402.0
49	69023.0
50	69940.0
51	70857.0
52	70648.0
53	70439.0
54	70439.0
55	68460.0
56	66481.0
57	64024.5
58	61568.0
59	59212.0
60	56856.0
61	56856.0
62	52124.0
63	47392.0
64	43040.0
65	38688.0
66	32788.5
67	26889.0
68	26889.0
69	23474.0
70	20059.0
71	16549.0
72	13039.0
73	9375.5
74	5712.0
75	5712.0
76	4302.5
77	2893.0
78	2270.0
79	1647.0
80	1297.0
81	947.0
82	947.0
83	710.0
84	473.0
85	356.5
86	240.0
87	175.5
88	111.0
89	111.0
90	91.5
91	72.0
92	47.5
93	23.0
94	17.0
95	11.0
96	11.0
97	7.0
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1130914.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.69750257038292
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.94869701369109	55.99168434850959
2	10.647839154996488	14.203685588188044
3	2.6476922026626495	5.297833724780247
4	0.9744698690526314	2.599788263835944
5	0.48789227721164297	1.6270598206696767
6	0.2685836457917691	1.0748315043335603
7	0.17575678084567609	0.8205776839551697
8	0.12466278140531256	0.6651756946569536
9	0.08882410683322071	0.5331911484438636
>10	0.553461349545666	6.961586657858376
>50	0.045212555020836674	2.0729336141645462
>100	0.033310709548291274	4.464275939666168
>500	0.0018653980565781169	0.7892506110368008
>1k	0.0014656699015970919	1.8505309620109562
>5k	2.664854366540167E-4	1.047594437890081
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6432	0.5687435118850771	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5356	0.47359923035703866	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4227	0.3737684739953701	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3239	0.28640550917222707	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2425	0.21442832965194525	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2063	0.18241882229771672	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1721	0.15217779601278258	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1362	0.12043356081894822	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	1218	0.10770049712002859	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	1158	0.10239505391214539	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1146	0.10133396527056877	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	1141	0.10089184500324516	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	8.842405346471969E-5	0.0	0.0	0.0	0.0
8	8.842405346471969E-5	0.0	0.0	0.0	0.0
9	8.842405346471969E-5	0.0	0.0	8.842405346471969E-5	0.0
10	8.842405346471969E-5	0.0	0.0	8.842405346471969E-5	0.0
11	8.842405346471969E-5	0.0	0.0	8.842405346471969E-5	0.0
12	1.7684810692943938E-4	0.0	0.0	1.7684810692943938E-4	0.0
13	1.7684810692943938E-4	0.0	0.0	1.7684810692943938E-4	0.0
14	1.7684810692943938E-4	0.0	0.0	1.7684810692943938E-4	0.0
15	2.6527216039415905E-4	0.0	0.0	2.6527216039415905E-4	0.0
16	2.6527216039415905E-4	0.0	0.0	4.421202673235984E-4	0.0
17	4.421202673235984E-4	0.0	0.0	4.421202673235984E-4	0.0
18	4.421202673235984E-4	0.0	0.0	6.189683742530379E-4	0.0
19	4.421202673235984E-4	0.0	0.0	7.073924277177575E-4	0.0
20	4.421202673235984E-4	0.0	0.0	9.726645881119165E-4	0.0
21	4.421202673235984E-4	0.0	0.0	0.0015916329623649544	0.0
22	4.421202673235984E-4	0.0	0.0	0.0026527216039415906	0.0
23	4.421202673235984E-4	0.0	0.0	0.004951746994024303	0.0
24	4.421202673235984E-4	0.0	0.0	0.010257190201907483	0.0
25	4.421202673235984E-4	0.0	0.0	0.018834323387985293	0.0
26	4.421202673235984E-4	0.0	0.0	0.03121369087304605	0.0
27	4.421202673235984E-4	0.0	0.0	0.10522462362301643	0.0
28	6.189683742530379E-4	0.0	0.0	0.2403365773171081	0.0
29	6.189683742530379E-4	0.0	0.0	0.4139129942683529	0.0
30	7.073924277177575E-4	0.0	0.0	0.6042015573244296	0.0
31	7.073924277177575E-4	0.0	0.0	0.9827449302068946	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1385	0.0	31.523466	1
TAGTCGG	45	0.0038257712	20.555555	30
GTATCAA	2285	0.0	19.269148	2
TTAGTCG	60	9.236692E-4	18.5	5
GGACCGC	70	1.2191752E-4	18.5	6
CGCCGTA	50	0.007034814	18.5	25
AACTCCG	70	0.0025924589	15.857143	7
ACCTAGG	430	0.0	14.627907	1
AACCACG	90	8.2738703E-4	14.388888	7
TCTGTCG	90	8.2738703E-4	14.388888	8
CTAGCAC	155	1.209828E-7	14.322581	3
GTATTAG	260	0.0	14.230769	1
CTACACT	145	8.90881E-7	14.034483	4
ATAGGAC	80	0.006298537	13.875001	3
GCTCTAT	95	0.0012450519	13.631579	1
TTAGTAC	95	0.0012450519	13.631579	3
GTACTAT	150	1.3053268E-6	13.566667	1
TCTATAC	150	1.3053268E-6	13.566667	3
ATCAACG	3275	0.0	13.500764	4
TATCAAC	3335	0.0	13.47976	3
>>END_MODULE