FastQCFastQC Report
Fri 10 Feb 2017
ERR1630091.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630091.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428214
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22140.5170312040241561No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19120.4465057191030653No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12260.28630544540813707No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9990.23329456766943632No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGCA250.005492541529.59999825
GGTATCA4700.029.5212751
GTAGAAC1151.3460522E-1020.9130423
GTCCCCG450.00382194720.55555711
GTAGTTA450.00382194720.5555576
TCAATAC555.135855E-420.1818183
GTATCAA7400.018.752
TGAACTG902.1458054E-618.55
TGTAGAA1306.9303496E-1018.52
CAGTACT609.223922E-418.54
AATTACG500.007027853318.49999816
GTATACC500.007027853318.49999835
TAGAACA953.5957055E-617.5263164
GCTACAC803.3763683E-416.18753
GTTATTC803.3763683E-416.18753
CCTACAC1151.2376713E-616.0869563
TAAGTCA957.04315E-515.5789484
TTGAACT1353.9549377E-715.0740754
CTGTAGA1252.948078E-614.8000011
TTTACAC750.00409881514.83
Produced by FastQC (version 0.11.5)