##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630091.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	428214
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.788792519628036	31.0	31.0	31.0	28.0	34.0
2	30.958149429957917	31.0	31.0	33.0	28.0	34.0
3	30.999089240426517	31.0	31.0	34.0	27.0	34.0
4	34.6601465622329	35.0	35.0	37.0	32.0	37.0
5	34.14504196499881	35.0	35.0	37.0	30.0	37.0
6	34.57400505354799	35.0	35.0	37.0	31.0	37.0
7	34.55896817946167	35.0	35.0	37.0	31.0	37.0
8	34.540299943486204	35.0	35.0	37.0	30.0	37.0
9	35.93393957226994	37.0	35.0	39.0	31.0	39.0
10	35.52761703260519	37.0	35.0	39.0	30.0	39.0
11	35.89883796419547	37.0	35.0	39.0	31.0	39.0
12	35.5610512500759	37.0	35.0	39.0	30.0	39.0
13	35.78608592899812	37.0	35.0	39.0	30.0	39.0
14	36.537112284979	38.0	35.0	40.0	31.0	41.0
15	36.533791515457224	38.0	35.0	40.0	31.0	41.0
16	36.368507335117485	38.0	35.0	40.0	30.0	41.0
17	36.24449223986138	38.0	35.0	40.0	30.0	41.0
18	36.067062263260894	38.0	34.0	40.0	29.0	41.0
19	36.10828230744441	38.0	34.0	40.0	29.0	41.0
20	36.236017505266055	38.0	34.0	40.0	30.0	41.0
21	36.19096993559295	38.0	34.0	40.0	30.0	41.0
22	36.14776256731447	38.0	34.0	40.0	29.0	41.0
23	36.04689477691061	38.0	34.0	40.0	29.0	41.0
24	35.94371739363963	38.0	34.0	40.0	29.0	41.0
25	35.892656475500566	38.0	34.0	40.0	29.0	41.0
26	35.56603006907761	38.0	34.0	40.0	27.0	41.0
27	35.3817857426427	38.0	34.0	40.0	27.0	41.0
28	35.28395148220282	38.0	34.0	40.0	27.0	41.0
29	35.056186859841105	38.0	33.0	40.0	27.0	41.0
30	34.896717529085926	37.0	33.0	40.0	26.0	41.0
31	34.69145333875119	37.0	33.0	39.0	25.0	40.0
32	34.54845240930936	37.0	33.0	39.0	25.0	40.0
33	34.39382178069844	37.0	33.0	39.0	25.0	40.0
34	34.285397021115614	37.0	33.0	39.0	25.0	40.0
35	34.07490647199764	37.0	32.0	39.0	24.0	40.0
36	34.01031026542803	37.0	32.0	39.0	24.0	40.0
37	33.92155557735151	37.0	32.0	39.0	24.0	40.0
38	33.751159466995475	37.0	31.0	39.0	24.0	40.0
39	33.63007281406026	36.0	31.0	39.0	23.0	40.0
40	33.50296347153526	36.0	31.0	39.0	23.0	40.0
41	33.28187541743147	36.0	31.0	39.0	22.0	40.0
42	33.11372351207574	36.0	31.0	39.0	21.0	40.0
43	32.75244620680314	35.0	30.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	0.0
13	3.0
14	9.0
15	19.0
16	40.0
17	82.0
18	149.0
19	286.0
20	471.0
21	772.0
22	1191.0
23	1798.0
24	2743.0
25	3867.0
26	5546.0
27	7493.0
28	9952.0
29	12790.0
30	16653.0
31	21347.0
32	26643.0
33	33654.0
34	41149.0
35	49903.0
36	56566.0
37	57252.0
38	49608.0
39	28224.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.31939170601615	25.425137898340548	14.788166664331387	21.467303731311915
2	21.384868313506797	25.753478400986424	32.05686876188074	20.804784523626036
3	21.697095377544873	26.585539006197834	30.61880274815863	21.09856286809866
4	17.932622473809822	19.816026566156175	33.97857146193258	28.272779498101418
5	13.84821607887645	39.041227050026386	34.455202305389356	12.6553545657078
6	32.24018831705642	38.793220212323746	14.997174310041242	13.96941716057859
7	26.694596626920188	32.760488914421295	22.672308705460352	17.87260575319817
8	25.68925817465099	35.98341016407684	20.293825050091776	18.033506611180393
9	25.476981135600425	14.74076046089105	21.060964844680463	38.721293558828066
10	15.98709990798993	29.08335551850243	34.99138281326626	19.938161760241375
11	34.336336504644876	23.203118067134657	22.667638143545048	19.792907284675422
12	19.629437617639777	27.478083388212436	31.24185570766019	21.650623286487598
13	29.488760292750822	21.834643425950574	25.968324249090408	22.708272032208196
14	21.02757032698604	23.162951234663044	27.31204491212338	28.49743352622754
15	23.854894982415335	30.180470512407346	23.740699743586152	22.223934761591167
16	22.575394545717796	28.91591587383878	26.093261780324788	22.415427800118632
17	21.934826979033847	28.647358563708803	27.654397100515165	21.763417356742192
18	21.923150574245586	27.64435539239726	28.284922959081204	22.147571074275945
19	22.995511589999392	27.25739933771432	29.239585814569352	20.507503257716937
20	22.944835993218344	27.110276637382242	28.842821579864275	21.10206578953514
21	22.510006678903537	27.484388646798095	28.45329671612792	21.552307958170445
22	22.45302582353683	27.457532915785098	28.607191731237187	21.482249529440885
23	22.519114274638383	27.364588733670548	28.408459321741	21.707837669950074
24	22.6015496924435	27.520585501641705	28.256199003302086	21.621665802612714
25	22.830874282484924	27.05983456869696	28.66253788993354	21.446753258884577
26	22.427104204906893	27.672145235793316	28.305473431508542	21.595277127791245
27	22.402817282947314	27.738934271182163	28.124956213482044	21.73329223238848
28	22.265736290733138	27.74547305786359	28.483188312385867	21.505602339017408
29	22.202683704876534	27.7064738658708	28.764122611591404	21.326719817661264
30	22.585202725739933	27.95004366975391	28.173063001209677	21.29169060329648
31	22.64475239016006	27.44445534242225	28.633580406058652	21.277211861359042
32	22.255928110710997	27.524321951173945	28.6949982952449	21.524751642870154
33	22.299831392714857	27.489993321096463	28.765056723974464	21.44511856221422
34	22.529155982756286	26.89099375545872	28.726991644364734	21.852858617420264
35	22.422900699183117	26.841485799156494	28.996483066877776	21.73913043478261
36	22.51257548795696	26.72355411079507	28.734698071524985	22.02917232972299
37	22.391140878159053	25.923253326607725	29.418935392117024	22.266670403116198
38	22.04411812785196	26.484654868827267	29.72088721994143	21.75033978337934
39	21.68401780418202	26.178499535279087	30.142171904701854	21.995310755837036
40	21.636144544550152	25.912511034202524	30.485224677380934	21.966119743866386
41	21.29916350236097	25.9958805643907	30.291162829800054	22.413793103448278
42	21.095527002853714	26.246456211146764	29.855165875006424	22.802850910993104
43	21.12892152054814	25.78080118819095	30.466775957815486	22.623501333445425
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	635.0
1	465.0
2	295.0
3	373.0
4	451.0
5	451.0
6	606.0
7	761.0
8	720.5
9	680.0
10	942.0
11	1204.0
12	1204.0
13	2068.0
14	2932.0
15	3994.5
16	5057.0
17	5027.5
18	4998.0
19	4998.0
20	6389.0
21	7780.0
22	7691.0
23	7602.0
24	8818.5
25	10035.0
26	10035.0
27	10969.5
28	11904.0
29	13544.0
30	15184.0
31	16490.0
32	17796.0
33	17796.0
34	19479.0
35	21162.0
36	22426.5
37	23691.0
38	24805.0
39	25919.0
40	25919.0
41	27291.5
42	28664.0
43	28153.5
44	27643.0
45	27800.5
46	27958.0
47	27958.0
48	27654.5
49	27351.0
50	27473.5
51	27596.0
52	27588.0
53	27580.0
54	27580.0
55	25462.0
56	23344.0
57	21456.0
58	19568.0
59	18058.5
60	16549.0
61	16549.0
62	14872.5
63	13196.0
64	11515.5
65	9835.0
66	8545.0
67	7255.0
68	7255.0
69	6170.0
70	5085.0
71	4302.5
72	3520.0
73	2832.5
74	2145.0
75	2145.0
76	1729.5
77	1314.0
78	1033.5
79	753.0
80	560.5
81	368.0
82	368.0
83	298.0
84	228.0
85	168.0
86	108.0
87	75.0
88	42.0
89	42.0
90	30.5
91	19.0
92	12.5
93	6.0
94	3.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	428214.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.64770712899242
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.84208051546628	66.16663233662418
2	6.3219186654570185	9.311896287337783
3	1.506644234961661	3.3288268009212376
4	0.631536634880498	1.8604490050763336
5	0.3558559322304123	1.3103986738509987
6	0.22837118569403922	1.0091408520417207
7	0.17132882985145828	0.8832582838557222
8	0.12601673017237514	0.7424674589670678
9	0.10772617882372827	0.7140407461321865
>10	0.6429635585700172	9.008160113375032
>50	0.049567891534667945	2.4663664906914446
>100	0.014710470969233782	1.702574799240986
>500	3.197928471572561E-4	0.2352845793943485
>1k	9.593785414717682E-4	1.260503572491044
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2214	0.5170312040241561	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1912	0.4465057191030653	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1226	0.28630544540813707	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	999	0.23329456766943632	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	2.335280957652015E-4	0.0	0.0	0.0
6	0.0	2.335280957652015E-4	0.0	0.0	0.0
7	0.0	2.335280957652015E-4	0.0	0.0	0.0
8	0.0	2.335280957652015E-4	0.0	0.0	0.0
9	0.0	2.335280957652015E-4	0.0	0.0	0.0
10	0.0	2.335280957652015E-4	0.0	2.335280957652015E-4	0.0
11	0.0	4.67056191530403E-4	0.0	4.67056191530403E-4	0.0
12	0.0	4.67056191530403E-4	0.0	7.005842872956045E-4	0.0
13	0.0	4.67056191530403E-4	0.0	7.005842872956045E-4	0.0
14	0.0	4.67056191530403E-4	0.0	7.005842872956045E-4	0.0
15	0.0	4.67056191530403E-4	0.0	7.005842872956045E-4	0.0
16	0.0	4.67056191530403E-4	0.0	7.005842872956045E-4	0.0
17	0.0	4.67056191530403E-4	0.0	7.005842872956045E-4	0.0
18	0.0	4.67056191530403E-4	0.0	7.005842872956045E-4	0.0
19	0.0	4.67056191530403E-4	0.0	7.005842872956045E-4	0.0
20	0.0	4.67056191530403E-4	0.0	9.34112383060806E-4	0.0
21	0.0	4.67056191530403E-4	0.0	9.34112383060806E-4	0.0
22	0.0	4.67056191530403E-4	0.0	9.34112383060806E-4	0.0
23	0.0	7.005842872956045E-4	0.0	0.0016346966703564105	0.0
24	0.0	7.005842872956045E-4	0.0	0.0058382023941300374	0.0
25	0.0	7.005842872956045E-4	0.0	0.011909932884025276	0.0
26	0.0	7.005842872956045E-4	0.0	0.021017528618868134	0.0
27	0.0	7.005842872956045E-4	0.0	0.07075901301685605	0.0
28	0.0	7.005842872956045E-4	0.0	0.15202679034314617	0.0
29	0.0	9.34112383060806E-4	0.0	0.25034211866029604	0.0
30	0.0	9.34112383060806E-4	0.0	0.3507591998393327	0.0
31	0.0	9.34112383060806E-4	0.0	0.5611680141237793	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGCA	25	0.0054925415	29.599998	25
GGTATCA	470	0.0	29.521275	1
GTAGAAC	115	1.3460522E-10	20.913042	3
GTCCCCG	45	0.003821947	20.555557	11
GTAGTTA	45	0.003821947	20.555557	6
TCAATAC	55	5.135855E-4	20.181818	3
GTATCAA	740	0.0	18.75	2
TGAACTG	90	2.1458054E-6	18.5	5
TGTAGAA	130	6.9303496E-10	18.5	2
CAGTACT	60	9.223922E-4	18.5	4
AATTACG	50	0.0070278533	18.499998	16
GTATACC	50	0.0070278533	18.499998	35
TAGAACA	95	3.5957055E-6	17.526316	4
GCTACAC	80	3.3763683E-4	16.1875	3
GTTATTC	80	3.3763683E-4	16.1875	3
CCTACAC	115	1.2376713E-6	16.086956	3
TAAGTCA	95	7.04315E-5	15.578948	4
TTGAACT	135	3.9549377E-7	15.074075	4
CTGTAGA	125	2.948078E-6	14.800001	1
TTTACAC	75	0.004098815	14.8	3
>>END_MODULE