FastQCFastQC Report
Fri 10 Feb 2017
ERR1630090.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630090.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences946274
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT37590.39724223639241907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30520.3225281472385377No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24390.25774775593538446No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17550.18546425242583015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6950.031.6762581
GTATCAA12750.017.4117642
CTGTTCG650.001579654917.0769239
ATTAGAC904.4446213E-516.4444453
TAATCTA1352.2177119E-816.44444320
CGAATTA1251.656681E-716.27999915
CGTTTAG803.381774E-416.187526
TACGAGT957.057568E-515.5789484
ATCTCGC1752.235538E-914.811
CTACACT1508.094503E-814.84
AGTACCG750.004103692214.85
TTCCTCG1752.235538E-914.819
GCTGCGT908.272111E-414.3888888
AATTCGG908.272111E-414.38888817
GGATAAG1551.2091186E-714.3225811
TGCGTTT1304.441672E-614.23076924
AGATCCG800.00629753513.8750015
ACGTTGA800.00629753513.87500131
GTCGTTG800.00629753513.8750011
ATAATAC2151.9645086E-1013.7674423
Produced by FastQC (version 0.11.5)