FastQCFastQC Report
Fri 10 Feb 2017
ERR1630089.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630089.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315420
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13300.42166000887705285No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10800.3424006087121933No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8230.2609219453427176No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6490.2057574028279754No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC5100.1616891763363135No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAGT250.005490374329.59999812
GGTATCA3000.028.9833341
GCACCGA903.801688E-922.611116
TGCACCG957.0958777E-921.4210535
CCGAGAG1059.749783E-1021.1428579
CGAGAGA1052.2439053E-819.3809510
ACCGAGA1103.8253347E-818.58
GCATTAT500.007023846718.4999981
TGCGTTA500.007023846718.49999837
GTATCAA4850.017.9278352
GATGGAA1156.36137E-817.69565217
CCAGGAC2000.017.5749993
TGCGTCT953.58963E-617.52631637
GTCAGAT650.001576499917.0769232
AGAGACC650.001576499917.0769238
TGCATCA1901.8189894E-1216.55263114
GAGAGAT1251.6464219E-716.2813
ACGGGAG803.3728412E-416.18755
TAGGACA803.3728412E-416.18754
TCCAGGA2800.015.8571432
Produced by FastQC (version 0.11.5)