##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630089.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	315420
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.72933865956502	34.0	31.0	34.0	31.0	34.0
2	32.97540422294084	34.0	31.0	34.0	31.0	34.0
3	33.10548475049141	34.0	33.0	34.0	31.0	34.0
4	36.43307336250079	37.0	37.0	37.0	35.0	37.0
5	36.37279817386342	37.0	37.0	37.0	35.0	37.0
6	36.423800012681504	37.0	37.0	37.0	35.0	37.0
7	36.35331304292689	37.0	37.0	37.0	35.0	37.0
8	36.35617589246085	37.0	37.0	37.0	35.0	37.0
9	38.10800202904064	39.0	38.0	39.0	37.0	39.0
10	38.07548665271701	39.0	38.0	39.0	37.0	39.0
11	38.117243675099864	39.0	38.0	39.0	37.0	39.0
12	38.0513917950669	39.0	38.0	39.0	35.0	39.0
13	38.062786126434595	39.0	38.0	39.0	37.0	39.0
14	39.479050155348425	41.0	39.0	41.0	37.0	41.0
15	39.47144759368461	41.0	39.0	41.0	37.0	41.0
16	39.39779341829941	41.0	39.0	41.0	36.0	41.0
17	39.31535096062393	41.0	39.0	41.0	36.0	41.0
18	39.18012491281466	40.0	39.0	41.0	36.0	41.0
19	39.21225667364149	40.0	39.0	41.0	36.0	41.0
20	39.42960497114958	41.0	39.0	41.0	37.0	41.0
21	39.44260668315262	41.0	39.0	41.0	37.0	41.0
22	39.45215585568448	41.0	39.0	41.0	37.0	41.0
23	39.3837010969501	41.0	39.0	41.0	36.0	41.0
24	39.32924671866083	41.0	39.0	41.0	36.0	41.0
25	39.30655316720563	41.0	39.0	41.0	36.0	41.0
26	39.14071079830068	41.0	39.0	41.0	36.0	41.0
27	39.05064992708135	40.0	39.0	41.0	35.0	41.0
28	38.99816752266819	40.0	39.0	41.0	35.0	41.0
29	38.878799695643906	40.0	39.0	41.0	35.0	41.0
30	38.7827404730201	40.0	39.0	41.0	35.0	41.0
31	38.6546636231057	40.0	38.0	41.0	35.0	41.0
32	38.57650434341513	40.0	38.0	41.0	35.0	41.0
33	38.47282987762349	40.0	38.0	41.0	35.0	41.0
34	38.383716948830134	40.0	38.0	41.0	35.0	41.0
35	38.236354701667615	40.0	38.0	41.0	34.0	41.0
36	38.20974256546827	40.0	38.0	41.0	34.0	41.0
37	38.14625261556021	40.0	38.0	41.0	34.0	41.0
38	38.042321349312026	40.0	38.0	41.0	34.0	41.0
39	37.99291103924926	40.0	38.0	41.0	34.0	41.0
40	37.90568448417982	40.0	38.0	41.0	33.0	41.0
41	37.78412909771099	40.0	38.0	41.0	33.0	41.0
42	37.689455329402065	40.0	38.0	41.0	33.0	41.0
43	37.376212668822525	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	6.0
17	6.0
18	8.0
19	22.0
20	48.0
21	101.0
22	119.0
23	253.0
24	373.0
25	582.0
26	836.0
27	1174.0
28	1648.0
29	2092.0
30	2728.0
31	3374.0
32	4482.0
33	5930.0
34	8031.0
35	11159.0
36	16772.0
37	29970.0
38	65544.0
39	160161.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.792784224208994	19.486716124532368	12.455456217107349	25.26504343415129
2	18.988967091497052	21.06049077420582	35.42958594889354	24.520956185403588
3	19.252108300044384	23.209688669076154	31.05636928539725	26.481833745482213
4	14.37892334030816	15.94286982436117	35.90609346268467	33.772113372646
5	14.059983514044767	36.894933739141464	34.788535920360154	14.256546826453617
6	33.597742692283305	36.13309238475683	15.425781497685625	14.843383425274236
7	27.965886754169045	30.892460845856316	22.412973178618984	18.728679221355655
8	26.081415255849343	34.5498066070636	20.27582271257371	19.09295542451335
9	25.590324012427875	14.831653034049838	20.685752330226364	38.89227062329592
10	16.867034430283432	27.135882315642633	34.10785619174434	21.889227062329592
11	34.02479234037157	22.45450510430537	22.625388371060808	20.895314184262254
12	20.736161308731216	25.83539407773762	30.69938494705472	22.729059666476445
13	29.29744467693868	20.598884027645678	25.652146344556463	24.45152495085917
14	21.27607634265424	21.90698116796652	26.87813074630651	29.93881174307273
15	24.10246655253313	28.4883647200558	23.56540485701604	23.84376387039503
16	23.102846997653923	27.608268340625198	25.264409358949973	24.02447530277091
17	22.40219390019656	27.620315769450254	27.12922452602879	22.848265804324395
18	22.413607253820302	26.346141652399975	27.612072791833107	23.628178301946612
19	23.373914146217743	26.024665525331304	28.28038805402321	22.321032274427747
20	23.428761651131826	26.268467440238414	27.915477775664193	22.387293132965567
21	23.637689429966393	26.191427303278168	27.193583158962653	22.977300107792782
22	23.253122820366496	26.52399974636992	27.586392746179694	22.63648468708389
23	23.332065182930695	26.367383171644153	27.214824678206835	23.085726967218314
24	23.261999873184962	26.30270750110963	27.43770211147042	22.99759051423499
25	23.372328958214446	25.9926447276647	27.791516073806356	22.8435102403145
26	23.142793735337012	26.69710227632997	27.51727854923594	22.642825439097077
27	23.15579227696405	26.79950542134297	27.10005706676812	22.944645234924863
28	22.92467186608332	26.078878955044065	27.93545114450574	23.060998034366875
29	22.69164922959863	26.359140194027013	28.19478790184516	22.7544226745292
30	22.879018451588358	26.571555386468837	27.654555830321474	22.89487033162133
31	22.846997653921754	26.527487159977177	27.717329275252045	22.908185910849028
32	22.575296430156616	26.226301439350706	28.173863420201634	23.02453871029104
33	22.779468644981296	26.33124088516898	27.869190285967914	23.02010018388181
34	23.18971530023461	25.891826770655	27.668188447149834	23.25026948196056
35	22.930378542895188	25.995815103671294	28.149134487350196	22.92467186608332
36	23.118064802485573	25.759939128780672	27.51220594762539	23.60979012110836
37	22.38761017056623	24.82436116923467	28.95599518102847	23.83203347917063
38	22.62919282226872	24.930885803056242	29.35482848265804	23.08509289201699
39	21.599137657726207	25.391224399213748	29.509859869380506	23.49977807367954
40	21.814089150973306	24.423308604400482	30.36586139116099	23.39674085346522
41	21.108680489506053	24.625578593621203	30.387419948005835	23.878320968866905
42	20.81668885929871	25.218121869253697	29.9546636231057	24.010525648341893
43	20.79608141525585	24.580559254327564	30.530403905903242	24.092955424513345
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	425.0
1	330.0
2	235.0
3	380.0
4	525.0
5	525.0
6	569.0
7	613.0
8	547.0
9	481.0
10	625.5
11	770.0
12	770.0
13	1295.0
14	1820.0
15	2276.5
16	2733.0
17	2791.5
18	2850.0
19	2850.0
20	3834.5
21	4819.0
22	4706.5
23	4594.0
24	5422.0
25	6250.0
26	6250.0
27	6738.0
28	7226.0
29	8155.0
30	9084.0
31	10238.0
32	11392.0
33	11392.0
34	12613.0
35	13834.0
36	15085.0
37	16336.0
38	16928.5
39	17521.0
40	17521.0
41	18253.0
42	18985.0
43	19477.0
44	19969.0
45	19930.5
46	19892.0
47	19892.0
48	19830.5
49	19769.0
50	20171.0
51	20573.0
52	20558.0
53	20543.0
54	20543.0
55	19919.5
56	19296.0
57	18175.0
58	17054.0
59	15587.0
60	14120.0
61	14120.0
62	13140.0
63	12160.0
64	10503.5
65	8847.0
66	7645.0
67	6443.0
68	6443.0
69	5475.0
70	4507.0
71	3929.0
72	3351.0
73	3366.5
74	3382.0
75	3382.0
76	3043.0
77	2704.0
78	2020.5
79	1337.0
80	926.0
81	515.0
82	515.0
83	368.0
84	221.0
85	174.0
86	127.0
87	92.0
88	57.0
89	57.0
90	47.5
91	38.0
92	26.5
93	15.0
94	10.0
95	5.0
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	315420.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.15917965238523
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.60099231187884	65.55135093576733
2	6.463004957384726	9.45656281543131
3	1.5312117558461702	3.3606658779538257
4	0.6587731592312844	1.9278121562548367
5	0.3816271542580231	1.3959764769295622
6	0.24498307269107097	1.0753656376079648
7	0.18249600251371745	0.9345880483620241
8	0.12946217156613027	0.7577077014235555
9	0.10613824011037336	0.6988487918600523
>10	0.6315390214174269	8.644290749392477
>50	0.04578797547335445	2.313921636007631
>100	0.021803797844442887	2.481731201778039
>500	0.0013082278706665735	0.6323166527733738
>1k	8.721519137777155E-4	0.7688613184580376
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1330	0.42166000887705285	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1080	0.3424006087121933	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	823	0.2609219453427176	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	649	0.2057574028279754	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	510	0.1616891763363135	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	3.170376006594382E-4	0.0	0.0	0.0	0.0
6	3.170376006594382E-4	0.0	0.0	0.0	0.0
7	9.511128019783146E-4	0.0	0.0	0.0	0.0
8	9.511128019783146E-4	0.0	0.0	0.0	0.0
9	9.511128019783146E-4	0.0	0.0	3.170376006594382E-4	0.0
10	0.001585188003297191	0.0	0.0	9.511128019783146E-4	0.0
11	0.0019022256039566293	0.0	0.0	9.511128019783146E-4	0.0
12	0.0019022256039566293	0.0	0.0	9.511128019783146E-4	0.0
13	0.0019022256039566293	0.0	0.0	9.511128019783146E-4	0.0
14	0.0019022256039566293	0.0	0.0	9.511128019783146E-4	0.0
15	0.0025363008052755056	0.0	0.0	9.511128019783146E-4	0.0
16	0.0025363008052755056	0.0	0.0	9.511128019783146E-4	0.0
17	0.003170376006594382	0.0	0.0	0.0012681504026377528	0.0
18	0.00348741360725382	0.0	0.0	0.0012681504026377528	0.0
19	0.0038044512079132586	0.0	0.0	0.0012681504026377528	0.0
20	0.0038044512079132586	0.0	0.0	0.0019022256039566293	0.0
21	0.004121488808572697	0.0	0.0	0.002853338405934944	0.0
22	0.004121488808572697	0.0	0.0	0.004755564009891573	0.0
23	0.004121488808572697	0.0	0.0	0.00697482721450764	0.0
24	0.004121488808572697	0.0	0.0	0.013632616828355842	0.0
25	0.004121488808572697	0.0	0.0	0.021875594445501235	0.0
26	0.004438526409232135	0.0	0.0	0.038044512079132585	0.0
27	0.004438526409232135	0.0	0.0	0.1211083634519054	0.0
28	0.005072601610551011	0.0	0.0	0.2609219453427176	0.0
29	0.005072601610551011	0.0	0.0	0.4108807304546319	0.0
30	0.005072601610551011	0.0	0.0	0.5871536364212796	0.0
31	0.005072601610551011	0.0	0.0	0.8959482594635724	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAAGT	25	0.0054903743	29.599998	12
GGTATCA	300	0.0	28.983334	1
GCACCGA	90	3.801688E-9	22.61111	6
TGCACCG	95	7.0958777E-9	21.421053	5
CCGAGAG	105	9.749783E-10	21.142857	9
CGAGAGA	105	2.2439053E-8	19.38095	10
ACCGAGA	110	3.8253347E-8	18.5	8
GCATTAT	50	0.0070238467	18.499998	1
TGCGTTA	50	0.0070238467	18.499998	37
GTATCAA	485	0.0	17.927835	2
GATGGAA	115	6.36137E-8	17.695652	17
CCAGGAC	200	0.0	17.574999	3
TGCGTCT	95	3.58963E-6	17.526316	37
GTCAGAT	65	0.0015764999	17.076923	2
AGAGACC	65	0.0015764999	17.076923	8
TGCATCA	190	1.8189894E-12	16.552631	14
GAGAGAT	125	1.6464219E-7	16.28	13
ACGGGAG	80	3.3728412E-4	16.1875	5
TAGGACA	80	3.3728412E-4	16.1875	4
TCCAGGA	280	0.0	15.857143	2
>>END_MODULE