FastQCFastQC Report
Fri 10 Feb 2017
ERR1630088.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630088.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79679
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13291.6679426197617944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12961.6265264373297856No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8511.0680354924133084No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6280.7881625020394332No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6210.7793772512205224No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5420.6802294205499567No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2320.291168312855332No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2260.2836380978676941No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.24473198709823166No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1730.21712119881022604No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1460.1832352313658555No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC1140.14307408476512004No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACA1010.12675861895857127No Hit
GAGTACGGGGGAGGCAGTACAGGCACTAAAAAAAAAAAAAAAA840.10542300982693056No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGAA200.001831671137.027
CACAAAA358.796825E-426.42857223
GGTATCA3650.025.8493161
CTATAGA450.00379509720.55555711
TAATGTT450.00379509720.55555723
AATGTTA450.00379509720.55555724
GTTGAGC555.085572E-420.1818183
TGTTAGT555.085572E-420.18181826
CCCTATA656.7996356E-519.9230779
TATACAC656.7996356E-519.92307737
CTATCAC500.006978952318.53
ATCACCC500.006978952318.55
AGAACTA609.13435E-418.518
ACTAATG500.006978952318.521
TCTCACT500.006978952318.52
AACTAAT500.006978952318.520
CCTATAG500.006978952318.510
CTAATGT500.006978952318.522
TCTATCA500.006978952318.52
TTATACA752.0370027E-417.26666636
Produced by FastQC (version 0.11.5)