##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630088.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	79679
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73339273836268	33.0	31.0	34.0	31.0	34.0
2	32.92390717754992	34.0	31.0	34.0	31.0	34.0
3	33.01805996561202	34.0	33.0	34.0	31.0	34.0
4	36.306304044980486	37.0	37.0	37.0	35.0	37.0
5	36.32128917280588	37.0	37.0	37.0	35.0	37.0
6	36.43013843045219	37.0	37.0	37.0	35.0	37.0
7	36.346992306630355	37.0	37.0	37.0	35.0	37.0
8	36.37890786781963	37.0	37.0	37.0	35.0	37.0
9	38.124374050879155	39.0	39.0	39.0	37.0	39.0
10	38.11804867028954	39.0	39.0	39.0	37.0	39.0
11	38.15995431669574	39.0	39.0	39.0	37.0	39.0
12	38.08801566284718	39.0	39.0	39.0	37.0	39.0
13	38.127775197981904	39.0	39.0	39.0	37.0	39.0
14	39.453745654438436	41.0	39.0	41.0	37.0	41.0
15	39.46551789053577	41.0	39.0	41.0	37.0	41.0
16	39.33281040173697	41.0	39.0	41.0	36.0	41.0
17	39.31616862661429	41.0	39.0	41.0	36.0	41.0
18	39.249325418240694	41.0	39.0	41.0	36.0	41.0
19	39.27367311336739	41.0	39.0	41.0	36.0	41.0
20	39.460146337177925	41.0	39.0	41.0	36.0	41.0
21	39.431481318791654	41.0	39.0	41.0	36.0	41.0
22	39.387818622221666	41.0	39.0	41.0	36.0	41.0
23	39.30373122152638	41.0	39.0	41.0	35.0	41.0
24	39.2226182557512	41.0	39.0	41.0	35.0	41.0
25	39.1764831385936	41.0	39.0	41.0	35.0	41.0
26	38.93476323749043	41.0	38.0	41.0	35.0	41.0
27	38.77756999962349	40.0	38.0	41.0	35.0	41.0
28	38.7079782627794	40.0	38.0	41.0	35.0	41.0
29	38.538197015524794	40.0	38.0	41.0	35.0	41.0
30	38.324602467400446	40.0	37.0	41.0	35.0	41.0
31	38.06107004354974	40.0	37.0	41.0	35.0	41.0
32	37.84436300656384	40.0	36.0	41.0	34.0	41.0
33	37.67911243866013	40.0	35.0	41.0	34.0	41.0
34	37.56405075364901	40.0	35.0	41.0	34.0	41.0
35	37.32799106414488	40.0	35.0	41.0	33.0	41.0
36	37.168375607123586	40.0	35.0	41.0	33.0	41.0
37	36.99769073407046	40.0	35.0	41.0	33.0	41.0
38	36.763814806912734	40.0	35.0	41.0	32.0	41.0
39	36.58543656421391	40.0	35.0	41.0	31.0	41.0
40	36.36530327940863	40.0	35.0	41.0	30.0	41.0
41	36.12818935980622	40.0	35.0	41.0	29.0	41.0
42	35.871496881235956	39.0	35.0	41.0	27.0	41.0
43	35.4354723327351	39.0	35.0	41.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	2.0
17	0.0
18	2.0
19	2.0
20	7.0
21	22.0
22	42.0
23	70.0
24	124.0
25	209.0
26	287.0
27	438.0
28	554.0
29	774.0
30	1032.0
31	1196.0
32	1531.0
33	1855.0
34	2498.0
35	3562.0
36	6055.0
37	10322.0
38	14716.0
39	34378.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.62933771759184	19.78312980835603	13.775273284052258	20.812259189999875
2	20.302714642503044	23.467915008973506	33.353832251910795	22.875538096612658
3	20.50728548300054	24.743031413546856	32.002158661629785	22.747524441822815
4	13.581997766036219	17.57426674531558	35.69949422056	33.1442412680882
5	12.695942469157496	38.76429172052862	34.55992168576413	13.979844124549757
6	30.860076055171376	36.80643582374277	14.815697988177561	17.517790132908296
7	26.707162489489072	31.828963716914117	22.45133598564239	19.012537807954416
8	27.748842229445653	32.91958985429034	19.726653195948746	19.604914720315264
9	24.469433602329346	14.190690144203616	21.855193965787723	39.48468228767931
10	19.012537807954416	27.48026456155323	31.575446478996977	21.931751151495373
11	33.0701941540431	23.067558578797424	21.945556545639377	21.916690721520098
12	20.585097704539464	30.258913891991618	28.887159728410243	20.26882867505867
13	31.313143990260922	20.947803059777357	25.437066228240816	22.301986721720905
14	20.73570200429222	23.687546279446277	29.980295937449014	25.596455778812484
15	24.35271527002096	28.672548601262566	26.054543857227124	20.920192271489352
16	21.282897626727244	26.88663261336111	28.38389036006978	23.446579399841866
17	20.6114534569962	28.676313708756386	28.469232796596344	22.243000037651075
18	21.237716336801416	25.86377841087363	29.892443429259906	23.00606182306505
19	23.10269958207307	25.77969101017834	31.493869149964233	19.62374025778436
20	22.663437041127523	26.15243665206642	31.511439651602053	19.672686655204004
21	21.441032141467637	26.92804879579312	31.471278505001315	20.159640557737923
22	21.141078577793397	27.138894815446978	31.797587821132296	19.92243878562733
23	20.91140702067044	26.48627618318503	31.857829541033393	20.74448725511113
24	20.088103515355364	28.20944037952284	30.870116341821557	20.832339763300244
25	20.25878838840849	27.22298221614227	31.390956211799846	21.127273183649393
26	19.887297782351688	28.35376950011923	31.825198609420298	19.933734108108787
27	19.702807515154557	27.702405903688547	31.624392876416625	20.97039370474027
28	20.079318264536454	27.555566711429613	32.70497872714266	19.660136296891277
29	20.78464840171187	27.63086886130599	31.85155436187703	19.73292837510511
30	20.04543229709208	28.2483464902923	32.02098419909888	19.685237013516733
31	20.452063906424527	28.786756861908408	31.323184276911103	19.437994954755958
32	19.45556545639378	27.554311675598335	32.19417914381456	20.795943724193325
33	18.898329547308578	28.289762672724304	32.13268238808218	20.67922539188494
34	19.84211649242586	26.963189799068765	32.54558917657099	20.649104531934388
35	19.412894238130498	28.17304434041592	31.693419847136635	20.720641574316947
36	20.84740019327552	28.00988968235043	30.83372030271464	20.30898982165941
37	19.1154507461188	27.45767391659032	31.67082920217372	21.756046135117156
38	19.33257194492903	26.69335709534507	32.87315352853324	21.100917431192663
39	18.279596882490996	26.993310659019315	33.340026857766794	21.387065600722902
40	18.3862749281492	27.21921710864845	33.952484343428004	20.442023619774343
41	18.090086471968775	26.86027686090438	33.454235118412626	21.595401548714214
42	17.714830758418152	27.207921786166995	33.12416069478783	21.953086760627016
43	18.06749582700586	26.102235218815494	33.449214975087536	22.381053979091103
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1299.0
1	754.5
2	210.0
3	291.5
4	373.0
5	373.0
6	424.0
7	475.0
8	422.0
9	369.0
10	496.5
11	624.0
12	624.0
13	1135.0
14	1646.0
15	2173.0
16	2700.0
17	2376.0
18	2052.0
19	2052.0
20	2257.0
21	2462.0
22	1756.5
23	1051.0
24	978.0
25	905.0
26	905.0
27	975.5
28	1046.0
29	1320.5
30	1595.0
31	1945.0
32	2295.0
33	2295.0
34	2706.5
35	3118.0
36	3571.0
37	4024.0
38	4192.0
39	4360.0
40	4360.0
41	4646.5
42	4933.0
43	5058.0
44	5183.0
45	5079.5
46	4976.0
47	4976.0
48	4840.0
49	4704.0
50	4594.0
51	4484.0
52	4286.5
53	4089.0
54	4089.0
55	4028.0
56	3967.0
57	3753.5
58	3540.0
59	3348.5
60	3157.0
61	3157.0
62	2924.5
63	2692.0
64	2431.0
65	2170.0
66	2018.5
67	1867.0
68	1867.0
69	1535.0
70	1203.0
71	1050.0
72	897.0
73	738.5
74	580.0
75	580.0
76	441.5
77	303.0
78	242.5
79	182.0
80	134.5
81	87.0
82	87.0
83	58.5
84	30.0
85	25.5
86	21.0
87	13.5
88	6.0
89	6.0
90	4.0
91	2.0
92	1.0
93	0.0
94	1.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	79679.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.7640783644373
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.33773596407454	51.47278454799884
2	9.79416007965375	12.098545413471555
3	2.6944100136142892	4.9925325368039255
4	1.229349968504257	3.037186711680618
5	0.7010342795602788	2.164936808945895
6	0.4287485014122285	1.5888753623915963
7	0.36575701542275413	1.5813451474039584
8	0.2357100766057749	1.164673251421328
9	0.16255867352122408	0.9036257985165476
>10	0.9570641903562067	10.271213243138092
>50	0.06705545282750493	2.624279923191807
>100	0.014223883933107105	1.4897275317210306
>500	0.008127933676061204	3.315804666223221
>1k	0.004063966838030602	3.2944690570915798
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1329	1.6679426197617944	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1296	1.6265264373297856	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	851	1.0680354924133084	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	628	0.7881625020394332	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	621	0.7793772512205224	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	542	0.6802294205499567	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	232	0.291168312855332	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	226	0.2836380978676941	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	195	0.24473198709823166	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	173	0.21712119881022604	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	146	0.1832352313658555	No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC	114	0.14307408476512004	No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACA	101	0.12675861895857127	No Hit
GAGTACGGGGGAGGCAGTACAGGCACTAAAAAAAAAAAAAAAA	84	0.10542300982693056	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0012550358312729828	0.0	0.0
10	0.0	0.0	0.0012550358312729828	0.0	0.0
11	0.0	0.0	0.0012550358312729828	0.0012550358312729828	0.0
12	0.0	0.0	0.0012550358312729828	0.0012550358312729828	0.0
13	0.0	0.0	0.0012550358312729828	0.0012550358312729828	0.0
14	0.0	0.0	0.0012550358312729828	0.0012550358312729828	0.0
15	0.0	0.0	0.0012550358312729828	0.0012550358312729828	0.0
16	0.0	0.0	0.0012550358312729828	0.0012550358312729828	0.0
17	0.0	0.0	0.0012550358312729828	0.0012550358312729828	0.0
18	0.0	0.0	0.0012550358312729828	0.0025100716625459656	0.0
19	0.0	0.0	0.0012550358312729828	0.0025100716625459656	0.0
20	0.0	0.0	0.0012550358312729828	0.0037651074938189486	0.0
21	0.0	0.0	0.0012550358312729828	0.005020143325091931	0.0
22	0.0	0.0	0.0025100716625459656	0.00878525081891088	0.0
23	0.0	0.0	0.0025100716625459656	0.010040286650183862	0.0
24	0.0	0.0	0.0025100716625459656	0.015060429975275794	0.0
25	0.0	0.0	0.0025100716625459656	0.02635575245673264	0.0
26	0.0	0.0	0.0025100716625459656	0.04518128992582738	0.0
27	0.0	0.0	0.0025100716625459656	0.13554386977748215	0.0
28	0.0	0.0	0.0025100716625459656	0.294933420349151	0.0
29	0.0	0.0	0.0025100716625459656	0.5170747624844689	0.0
30	0.0	0.0	0.0025100716625459656	0.7705920004016115	0.0
31	0.0	0.0	0.0025100716625459656	1.2173847563347933	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAGGAA	20	0.0018316711	37.0	27
CACAAAA	35	8.796825E-4	26.428572	23
GGTATCA	365	0.0	25.849316	1
CTATAGA	45	0.003795097	20.555557	11
TAATGTT	45	0.003795097	20.555557	23
AATGTTA	45	0.003795097	20.555557	24
GTTGAGC	55	5.085572E-4	20.181818	3
TGTTAGT	55	5.085572E-4	20.181818	26
CCCTATA	65	6.7996356E-5	19.923077	9
TATACAC	65	6.7996356E-5	19.923077	37
CTATCAC	50	0.0069789523	18.5	3
ATCACCC	50	0.0069789523	18.5	5
AGAACTA	60	9.13435E-4	18.5	18
ACTAATG	50	0.0069789523	18.5	21
TCTCACT	50	0.0069789523	18.5	2
AACTAAT	50	0.0069789523	18.5	20
CCTATAG	50	0.0069789523	18.5	10
CTAATGT	50	0.0069789523	18.5	22
TCTATCA	50	0.0069789523	18.5	2
TTATACA	75	2.0370027E-4	17.266666	36
>>END_MODULE