FastQCFastQC Report
Fri 10 Feb 2017
ERR1630087.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630087.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217966
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27571.2648761733481368No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23981.1001715863942083No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23711.087784333336392No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21811.0006147747813878No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16960.778103006890983No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14790.6785461952781626No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8930.40969692520851875No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7480.34317278841654203No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4970.22801721369387884No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATT4860.2229705550406944No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4160.1908554545204298No Hit
GTTGTAGTCCGTGCGAGAATACCAAAAAAAAAAAAAAAAAAAA3050.1399300808382959No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2500.11469678757237368No Hit
GAGTACGGGGAACCAGCAAAAAAAAAAAAAAAAAAAAAAAAAA2360.10827376746832074No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATT2270.1041446831157153No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACAG251.2303458E-437.037
TGTCCCC554.947651E-1033.63636410
GCTGTCC451.0731492E-732.888898
TACTGAC303.5899977E-430.83333434
TTGGTAA303.5899977E-430.83333433
GTAAACA303.5899977E-430.83333436
TTATAGA250.005486696529.628
AATTGTT250.005486696529.616
TGAATCC250.005486696529.635
CTAGGTT250.005486696529.622
GGTATCA5900.029.4745771
CTGTGCC453.9886945E-628.7777779
TACACTT358.8462577E-426.428575
TAGAGCT451.3184118E-424.6666664
TTAGAGC451.3184118E-424.6666663
GCTTAGA451.3184118E-424.6666661
AGCTGTG451.3184118E-424.6666667
GGTAAAC400.001926196923.12500235
TTATCAT400.001926196923.12500210
GTTACTC400.001926196923.12500228
Produced by FastQC (version 0.11.5)