FastQCFastQC Report
Fri 10 Feb 2017
ERR1630086.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630086.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690955
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29360.42491913366282896No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22730.32896498324782364No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18150.2626799140320281No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11920.17251485263150276No Hit
CTGTAGTCCTTTCTCTCAATTTTCTTTTAAATACATTTTTTAC7280.10536142006353526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6500.029.030771
AAGACCG400.001930411623.1250025
CTCCGCG450.003824287320.5555559
GCCGCTA450.003824287320.55555520
GTTTAAG2600.019.2115381
AGACCGT609.2317315E-418.56
GTATCAA10100.018.52
ATTGCCC2350.017.31914932
TATACTC752.0657631E-417.2666665
CGTAGTC803.3801227E-416.187528
TAGGACG803.3801227E-416.18754
TCGGTGT803.3801227E-416.187533
ATAGGAT700.0025910915.8571433
GACCGGG700.0025910915.8571437
AATCTAA2350.015.74468121
GTCCTAA957.053164E-515.5789461
CAAGACT1201.9326835E-615.4166674
TAAGTCA2800.015.1964294
TTAAGTC2600.014.9423073
TAAGGCT1001.0923612E-414.84
Produced by FastQC (version 0.11.5)