##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630086.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	690955
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83395155979767	34.0	31.0	34.0	31.0	34.0
2	33.05818758095679	34.0	33.0	34.0	31.0	34.0
3	33.192333798872575	34.0	33.0	34.0	31.0	34.0
4	36.4797215448184	37.0	37.0	37.0	35.0	37.0
5	36.42885281964817	37.0	37.0	37.0	35.0	37.0
6	36.47832492709366	37.0	37.0	37.0	35.0	37.0
7	36.409661989565166	37.0	37.0	37.0	35.0	37.0
8	36.41840206670478	37.0	37.0	37.0	35.0	37.0
9	38.18844642559935	39.0	39.0	39.0	37.0	39.0
10	38.16941479546425	39.0	39.0	39.0	37.0	39.0
11	38.21129451266725	39.0	39.0	39.0	37.0	39.0
12	38.153753862407825	39.0	39.0	39.0	37.0	39.0
13	38.162980223024654	39.0	39.0	39.0	37.0	39.0
14	39.60932477513008	41.0	40.0	41.0	37.0	41.0
15	39.60418840590198	41.0	40.0	41.0	37.0	41.0
16	39.51880947384417	41.0	39.0	41.0	37.0	41.0
17	39.456832934127405	41.0	39.0	41.0	37.0	41.0
18	39.359745569537814	41.0	39.0	41.0	36.0	41.0
19	39.38514519758884	41.0	39.0	41.0	36.0	41.0
20	39.6057760635642	41.0	40.0	41.0	37.0	41.0
21	39.611980519715466	41.0	40.0	41.0	37.0	41.0
22	39.62713056566636	41.0	40.0	41.0	37.0	41.0
23	39.555363229153855	41.0	40.0	41.0	37.0	41.0
24	39.52768414730337	41.0	40.0	41.0	37.0	41.0
25	39.50130761048115	41.0	40.0	41.0	37.0	41.0
26	39.360732609214786	41.0	39.0	41.0	36.0	41.0
27	39.27983587932644	41.0	39.0	41.0	36.0	41.0
28	39.23914871446042	41.0	39.0	41.0	36.0	41.0
29	39.120748818664026	41.0	39.0	41.0	36.0	41.0
30	39.04436757820697	40.0	39.0	41.0	35.0	41.0
31	38.906426612442196	40.0	39.0	41.0	35.0	41.0
32	38.81535555861091	40.0	39.0	41.0	35.0	41.0
33	38.739777554254616	40.0	38.0	41.0	35.0	41.0
34	38.6612890853963	40.0	38.0	41.0	35.0	41.0
35	38.53609569364141	40.0	38.0	41.0	35.0	41.0
36	38.50784783379525	40.0	38.0	41.0	35.0	41.0
37	38.46194035791043	40.0	38.0	41.0	35.0	41.0
38	38.35771359929373	40.0	38.0	41.0	35.0	41.0
39	38.299777843709066	40.0	38.0	41.0	34.0	41.0
40	38.24420693098682	40.0	38.0	41.0	34.0	41.0
41	38.134861170409074	40.0	38.0	41.0	34.0	41.0
42	38.03916318718296	40.0	38.0	41.0	34.0	41.0
43	37.73842290742523	40.0	38.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	0.0
15	3.0
16	4.0
17	9.0
18	15.0
19	26.0
20	60.0
21	139.0
22	224.0
23	405.0
24	654.0
25	1084.0
26	1421.0
27	2097.0
28	2929.0
29	3940.0
30	5127.0
31	6787.0
32	8605.0
33	11442.0
34	15495.0
35	21837.0
36	33078.0
37	60144.0
38	127931.0
39	387497.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.66413876446368	20.058614526271608	12.642357317046695	24.634889392218017
2	18.408868884370182	21.668560181198487	35.78105665347237	24.141514280958962
3	18.618578633919718	23.731936233184506	32.32149705841915	25.327988074476632
4	13.917983081387355	16.37993791202032	37.192581282427945	32.50949772416438
5	13.620279178817723	37.03642060626235	35.747045755512296	13.59625445940763
6	32.21989854621502	37.501718635801176	15.69711486276241	14.581267955221398
7	26.852399939214568	31.92002373526496	22.88803178209869	18.339544543421784
8	25.566643269098567	34.80704242678612	20.756199752516444	18.870114551598874
9	25.07891251962863	14.883024220101165	21.63064164815364	38.407421612116565
10	15.971807136499482	27.615546598548384	35.40114768689712	21.01149857805501
11	33.30954982596551	22.941725582707992	23.240587303080517	20.50813728824598
12	19.49316525678228	26.645729461397632	31.357179555832143	22.503925725987944
13	28.92793307813099	21.126122540541715	26.195917244972538	23.750027136354756
14	20.772119747306263	22.6499554963782	27.386153946349616	29.191770809965917
15	23.516003212944405	29.41001946581181	23.989116512652778	23.084860808591007
16	22.556751163245075	28.433544876294402	26.09127946103581	22.91842449942471
17	21.48938787620033	28.219059128307922	27.90818504823035	22.383367947261398
18	21.902584104608838	27.1024885846401	28.390126708685802	22.604800602065257
19	22.67398021578829	26.807679226577708	29.237359885954945	21.280980671679053
20	22.831298709756787	26.827072674776215	28.682909885593126	21.658718729873872
21	22.16989528985245	27.150248569009563	28.228321670731088	22.4515344704069
22	22.523608628637177	27.1645765643204	28.404020522320554	21.90779428472187
23	22.62694386754564	26.755577425447385	28.309513644159175	22.3079650628478
24	22.484532277789437	26.846755577425448	28.10168534853934	22.567026796245777
25	22.697426026296938	26.519816775332693	28.47320013604359	22.30955706232678
26	22.12416148663806	27.275727073398414	28.333683090794626	22.266428349168905
27	22.105925856242447	27.525381537147858	28.050162456310467	22.318530150299225
28	22.29667633926956	27.32189505828889	28.341498360964174	22.039930241477375
29	21.997525164446312	27.443465927592968	28.705197878298875	21.853811029661845
30	22.26208653240804	27.660701492861328	28.09589625952486	21.981315715205767
31	22.373671223162145	27.284844888596215	28.262043114240438	22.079440774001203
32	21.975816080642012	27.267332894327417	28.605770274475184	22.15108075055539
33	21.917346281595762	27.203074006266686	28.476528862226917	22.40305084991063
34	22.17119783488071	26.67612217872365	28.369285988233678	22.783393998161962
35	22.19305164591037	26.758471969954627	28.847030559153637	22.201445824981366
36	22.053100418985316	26.59695638645064	28.51415794082104	22.83578525374301
37	21.905912830792165	25.434652039568423	29.612492854093247	23.046942275546165
38	21.60155147585588	25.88895079997974	29.911354574465776	22.598143149698604
39	21.08328328183456	26.367997915927955	30.26231809596862	22.28640070626886
40	21.147252715444566	25.542908004139196	30.57854708338459	22.731292197031642
41	20.81741936884457	25.440296401357543	30.526445282254272	23.215838947543617
42	20.421735134704868	25.711370494460567	30.3579827919329	23.508911578901664
43	20.244733738087138	25.532053462237048	30.867277897981776	23.35593490169403
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	646.0
1	531.0
2	416.0
3	694.5
4	973.0
5	973.0
6	1160.0
7	1347.0
8	1168.5
9	990.0
10	1337.5
11	1685.0
12	1685.0
13	2737.0
14	3789.0
15	4984.0
16	6179.0
17	6564.5
18	6950.0
19	6950.0
20	9519.5
21	12089.0
22	12345.5
23	12602.0
24	14856.0
25	17110.0
26	17110.0
27	18288.0
28	19466.0
29	21523.5
30	23581.0
31	25812.0
32	28043.0
33	28043.0
34	30451.5
35	32860.0
36	35964.0
37	39068.0
38	40119.0
39	41170.0
40	41170.0
41	43209.5
42	45249.0
43	44904.5
44	44560.0
45	44431.5
46	44303.0
47	44303.0
48	43503.0
49	42703.0
50	42823.5
51	42944.0
52	42330.5
53	41717.0
54	41717.0
55	39478.5
56	37240.0
57	35180.5
58	33121.0
59	31060.0
60	28999.0
61	28999.0
62	26034.0
63	23069.0
64	20885.0
65	18701.0
66	16272.5
67	13844.0
68	13844.0
69	11823.0
70	9802.0
71	8093.5
72	6385.0
73	5265.5
74	4146.0
75	4146.0
76	3273.0
77	2400.0
78	1883.5
79	1367.0
80	1050.0
81	733.0
82	733.0
83	547.5
84	362.0
85	274.5
86	187.0
87	139.0
88	91.0
89	91.0
90	64.5
91	38.0
92	23.0
93	8.0
94	10.0
95	12.0
96	12.0
97	8.5
98	5.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	690955.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.60485576452896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.51109967015668	56.28849604032518
2	9.836918526365066	13.103730792319363
3	2.540013217935586	5.075316420617904
4	1.015317497774092	2.705003019777834
5	0.5316568014968907	1.7705462289965606
6	0.31053359400940195	1.240982714342221
7	0.20974124824026202	0.9778849910840444
8	0.15623564144912117	0.8324841891197903
9	0.1210188023995013	0.7254395890753335
>10	0.668715175001754	8.265998974704074
>50	0.05999453400072136	2.7393933997631867
>100	0.03568990090918899	4.198024327193157
>500	0.00218956447268843	0.8785138103646721
>1k	8.75825789075372E-4	1.1981855023167571
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2936	0.42491913366282896	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2273	0.32896498324782364	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1815	0.2626799140320281	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1192	0.17251485263150276	No Hit
CTGTAGTCCTTTCTCTCAATTTTCTTTTAAATACATTTTTTAC	728	0.10536142006353526	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.4472722536199899E-4	0.0
5	0.0	0.0	0.0	1.4472722536199899E-4	0.0
6	0.0	0.0	0.0	1.4472722536199899E-4	0.0
7	0.0	0.0	0.0	1.4472722536199899E-4	0.0
8	0.0	0.0	0.0	1.4472722536199899E-4	0.0
9	0.0	0.0	0.0	1.4472722536199899E-4	0.0
10	0.0	0.0	0.0	2.8945445072399797E-4	0.0
11	0.0	4.3418167608599693E-4	0.0	2.8945445072399797E-4	0.0
12	0.0	4.3418167608599693E-4	0.0	4.3418167608599693E-4	0.0
13	0.0	4.3418167608599693E-4	0.0	5.789089014479959E-4	0.0
14	0.0	4.3418167608599693E-4	0.0	5.789089014479959E-4	0.0
15	0.0	4.3418167608599693E-4	0.0	5.789089014479959E-4	0.0
16	0.0	4.3418167608599693E-4	0.0	5.789089014479959E-4	0.0
17	0.0	4.3418167608599693E-4	0.0	5.789089014479959E-4	0.0
18	0.0	4.3418167608599693E-4	0.0	7.236361268099948E-4	0.0
19	0.0	4.3418167608599693E-4	0.0	8.683633521719939E-4	0.0
20	0.0	4.3418167608599693E-4	0.0	0.0014472722536199897	0.0
21	0.0	4.3418167608599693E-4	0.0	0.0014472722536199897	0.0
22	0.0	4.3418167608599693E-4	0.0	0.0024603628311539827	0.0
23	0.0	7.236361268099948E-4	0.0	0.0037629078594119732	0.0
24	0.0	8.683633521719939E-4	0.0	0.0075258157188239465	0.0
25	0.0	8.683633521719939E-4	0.0	0.013604359184027904	0.0
26	0.0	8.683633521719939E-4	0.0	0.023301083283281836	0.0
27	0.0	8.683633521719939E-4	0.0	0.07757379279403145	0.0
28	0.0	0.0010130905775339928	0.0	0.17700139661772474	0.0
29	0.0	0.0010130905775339928	0.0	0.28424427061096597	0.0
30	0.0	0.0010130905775339928	0.0	0.39930241477375517	0.0
31	0.0	0.0010130905775339928	0.0	0.6427336078326374	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	650	0.0	29.03077	1
AAGACCG	40	0.0019304116	23.125002	5
CTCCGCG	45	0.0038242873	20.555555	9
GCCGCTA	45	0.0038242873	20.555555	20
GTTTAAG	260	0.0	19.211538	1
AGACCGT	60	9.2317315E-4	18.5	6
GTATCAA	1010	0.0	18.5	2
ATTGCCC	235	0.0	17.319149	32
TATACTC	75	2.0657631E-4	17.266666	5
CGTAGTC	80	3.3801227E-4	16.1875	28
TAGGACG	80	3.3801227E-4	16.1875	4
TCGGTGT	80	3.3801227E-4	16.1875	33
ATAGGAT	70	0.00259109	15.857143	3
GACCGGG	70	0.00259109	15.857143	7
AATCTAA	235	0.0	15.744681	21
GTCCTAA	95	7.053164E-5	15.578946	1
CAAGACT	120	1.9326835E-6	15.416667	4
TAAGTCA	280	0.0	15.196429	4
TTAAGTC	260	0.0	14.942307	3
TAAGGCT	100	1.0923612E-4	14.8	4
>>END_MODULE