FastQCFastQC Report
Fri 10 Feb 2017
ERR1630085.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630085.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1679306
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT69260.4124322785722197No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT58520.3484772876414423No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44220.26332306321778165No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30530.18180129172408124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15950.026.097181
CGTTATG450.00382653220.55555512
CGCGAAT1258.58563E-917.7628
GTATCAA24550.016.9551942
TTGCGCA803.3837266E-416.18759
AGACCGT1151.2429537E-616.0869566
GGCTACG2550.015.9607861
TCACGCT700.00259316115.8571424
GCTACGA2600.015.6538462
TACGCAT750.004105453414.828
ACCGTCT1152.2109334E-514.4782618
GTATTAG3550.014.0704221
TTAGGCA3950.014.0506344
TACGAGC2900.014.0344834
GCGTCTC2650.013.9622649
CGAATTG1601.7846469E-713.87500130
ATACGCC800.00630021513.8750016
CGAGCGT2551.8189894E-1213.7843156
CGCCGTA950.001245492513.631577525
GACTGCG950.001245492513.63157757
Produced by FastQC (version 0.11.5)