##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630085.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1679306
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.8693281629435	34.0	31.0	34.0	31.0	34.0
2	33.08789464219148	34.0	33.0	34.0	31.0	34.0
3	33.215103143798686	34.0	33.0	34.0	31.0	34.0
4	36.49504319046082	37.0	37.0	37.0	35.0	37.0
5	36.448045204388	37.0	37.0	37.0	35.0	37.0
6	36.49538082993808	37.0	37.0	37.0	35.0	37.0
7	36.425980137032795	37.0	37.0	37.0	35.0	37.0
8	36.43357017720415	37.0	37.0	37.0	35.0	37.0
9	38.21541279552387	39.0	39.0	39.0	37.0	39.0
10	38.19060790588493	39.0	39.0	39.0	37.0	39.0
11	38.23076199334725	39.0	39.0	39.0	37.0	39.0
12	38.1669785018335	39.0	39.0	39.0	37.0	39.0
13	38.17838261758131	39.0	39.0	39.0	37.0	39.0
14	39.63753240922143	41.0	40.0	41.0	37.0	41.0
15	39.62641174389897	41.0	40.0	41.0	37.0	41.0
16	39.54619348707144	41.0	39.0	41.0	37.0	41.0
17	39.47872871293261	41.0	39.0	41.0	37.0	41.0
18	39.375723066552496	41.0	39.0	41.0	36.0	41.0
19	39.401880896036815	41.0	39.0	41.0	36.0	41.0
20	39.62346767057344	41.0	40.0	41.0	37.0	41.0
21	39.63566973499767	41.0	40.0	41.0	37.0	41.0
22	39.64765563869837	41.0	40.0	41.0	37.0	41.0
23	39.57831092129725	41.0	40.0	41.0	37.0	41.0
24	39.54648527427401	41.0	40.0	41.0	37.0	41.0
25	39.518929248153704	41.0	40.0	41.0	37.0	41.0
26	39.3797163828391	41.0	39.0	41.0	36.0	41.0
27	39.29742643687333	41.0	39.0	41.0	36.0	41.0
28	39.25943217019412	41.0	39.0	41.0	36.0	41.0
29	39.1484529918907	41.0	39.0	41.0	36.0	41.0
30	39.054144986083536	41.0	39.0	41.0	35.0	41.0
31	38.92003006003671	40.0	39.0	41.0	35.0	41.0
32	38.830127445504274	40.0	39.0	41.0	35.0	41.0
33	38.74642917967303	40.0	38.0	41.0	35.0	41.0
34	38.67587682054373	40.0	38.0	41.0	35.0	41.0
35	38.54855398599183	40.0	38.0	41.0	35.0	41.0
36	38.52240687522107	40.0	38.0	41.0	35.0	41.0
37	38.47693451937884	40.0	38.0	41.0	35.0	41.0
38	38.37238597372962	40.0	38.0	41.0	35.0	41.0
39	38.31454541340292	40.0	38.0	41.0	35.0	41.0
40	38.24658996037649	40.0	38.0	41.0	34.0	41.0
41	38.13575846212662	40.0	38.0	41.0	34.0	41.0
42	38.03483879650284	40.0	38.0	41.0	34.0	41.0
43	37.729186937937456	40.0	38.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	0.0
14	3.0
15	9.0
16	11.0
17	24.0
18	49.0
19	91.0
20	176.0
21	327.0
22	631.0
23	1019.0
24	1614.0
25	2477.0
26	3688.0
27	5014.0
28	7009.0
29	9272.0
30	12245.0
31	15784.0
32	20377.0
33	26939.0
34	36943.0
35	52644.0
36	80609.0
37	145695.0
38	308081.0
39	948573.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.36493765877095	19.193881281910503	12.309787495548756	25.131393563769794
2	18.91823169809433	21.34697309483799	35.118614475265375	24.616180731802302
3	18.882562201290295	23.280033537663773	31.234212228146628	26.603192032899305
4	14.221946446925099	16.124101265641876	36.034409452476204	33.619542834956825
5	14.086235623525434	36.61232675879202	35.160238812938196	14.14119880474434
6	33.3126898849882	36.16154530502481	15.58834423267707	14.937420577309911
7	28.193194093274244	31.02311311934811	22.26735329951778	18.516339487859863
8	26.238577126503447	34.21258543707936	20.295407745818807	19.253429690598377
9	25.676737890533353	14.67606261157883	20.724275385188882	38.92292411269894
10	16.410171820978427	27.131326869552062	34.494785345851206	21.963715963618306
11	34.10003894465928	22.435994392921838	22.6488204055723	20.815146256846578
12	20.25163966543322	25.927377142700614	30.95373922322674	22.867243968639425
13	29.513977797971304	20.765959271270393	25.351544030688867	24.368518900069432
14	21.59427763611873	21.851705406876412	26.90730575606828	29.646711200936576
15	24.207738196612173	28.532441377569068	23.494646002574875	23.765174423243888
16	23.16951169113908	27.605391751116237	25.445273225963582	23.779823331781106
17	22.655430278936656	27.34290236562008	27.057725036413853	22.94394231902941
18	22.494113639801203	26.48754902322745	27.67059725862946	23.347740078341886
19	23.40538293795175	26.14996909437589	28.35195015083612	22.09269781683624
20	23.501851360026105	26.18813962434482	27.775640651554866	22.534368364074204
21	22.894338494592407	26.350170844384525	27.518510622840626	23.236980038182438
22	23.25270081807604	26.335462387438618	27.500169712964762	22.91166708152058
23	23.025404542114423	26.307295990129255	27.517141009440806	23.150158458315516
24	22.981457816502772	26.336117419934187	27.639691634520453	23.042733129042592
25	23.502149102069545	25.83275472129558	27.612954399019596	23.052141777615276
26	23.166534270704684	26.454321011179616	27.577642192667685	22.80150252544801
27	22.831753117061453	26.801369137012554	27.266144466821412	23.10073327910458
28	22.86789900113499	26.578360346476458	27.584907098527605	22.96883355386094
29	22.733379145909083	26.537271944481827	27.94279303474173	22.786555874867357
30	22.761069155948945	26.99245998049194	27.487247708279494	22.75922315527962
31	23.174632854286234	26.35481562026218	27.63105711526071	22.839494410190877
32	22.673652091995145	26.50761683695527	27.937969613638014	22.880761457411573
33	22.4995325449918	26.604085259029624	28.047479137215014	22.84890305876356
34	23.124493094171044	25.80589838897735	27.697870429808503	23.371738087043102
35	22.655251633710595	26.078868294402568	28.094105541217623	23.171774530669218
36	22.897792302296306	25.943336116228966	27.88967585419215	23.26919572728258
37	22.65578756938878	25.251443155684548	28.584010299492768	23.5087589754339
38	22.337679970178158	25.533583516047702	28.988343994483433	23.140392519290707
39	22.0128434007858	25.53138022492625	29.203551943481415	23.252224430806535
40	21.732906331544104	25.174804353703255	29.683095278644867	23.40919403610777
41	21.590883376823523	25.10745510347727	29.573288013024428	23.72837350667478
42	21.24300157326896	25.296938139922087	29.553220199296614	23.90684008751234
43	21.365849940392042	24.995325449918003	29.806003194176643	23.832821415513315
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1600.0
1	1315.0
2	1030.0
3	1834.0
4	2638.0
5	2638.0
6	2917.5
7	3197.0
8	2876.5
9	2556.0
10	3305.5
11	4055.0
12	4055.0
13	6571.0
14	9087.0
15	11822.5
16	14558.0
17	14824.5
18	15091.0
19	15091.0
20	19142.5
21	23194.0
22	22933.0
23	22672.0
24	26622.5
25	30573.0
26	30573.0
27	34398.0
28	38223.0
29	43327.0
30	48431.0
31	54978.5
32	61526.0
33	61526.0
34	67321.0
35	73116.0
36	79380.0
37	85644.0
38	88809.0
39	91974.0
40	91974.0
41	96417.5
42	100861.0
43	102426.0
44	103991.0
45	105716.0
46	107441.0
47	107441.0
48	108011.0
49	108581.0
50	111979.5
51	115378.0
52	116580.0
53	117782.0
54	117782.0
55	111816.0
56	105850.0
57	98886.0
58	91922.0
59	85257.0
60	78592.0
61	78592.0
62	72830.5
63	67069.0
64	58852.0
65	50635.0
66	43730.0
67	36825.0
68	36825.0
69	30581.0
70	24337.0
71	20705.0
72	17073.0
73	13695.0
74	10317.0
75	10317.0
76	8076.5
77	5836.0
78	4843.0
79	3850.0
80	2898.0
81	1946.0
82	1946.0
83	1447.0
84	948.0
85	717.5
86	487.0
87	363.0
88	239.0
89	239.0
90	176.5
91	114.0
92	76.5
93	39.0
94	26.5
95	14.0
96	14.0
97	10.5
98	7.0
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1679306.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.0408594967342
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.93933791790576	45.81707020858391
2	12.30369293271705	14.282338255975837
3	3.722608270870646	6.481901508329516
4	1.658318583235888	3.8500094356166996
5	0.870598808557109	2.526515156274368
6	0.5310452807131335	1.8493394714564855
7	0.36627622384469216	1.48812907916149
8	0.26282087499125245	1.2203479582540822
9	0.18905450815502536	0.9875597530545016
>10	1.015307727823241	10.788154903718777
>50	0.08998128206331613	3.620315956811624
>100	0.048281276828297616	4.922583452219134
>500	0.0020587017650691214	0.7937044861329792
>1k	4.1174035301382424E-4	0.6086163041201851
>5k	2.0587017650691212E-4	0.763414070290343
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6926	0.4124322785722197	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5852	0.3484772876414423	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4422	0.26332306321778165	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3053	0.18180129172408124	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.954840868787463E-5	0.0
3	0.0	0.0	0.0	1.1909681737574926E-4	0.0
4	0.0	5.954840868787463E-5	0.0	1.786452260636239E-4	0.0
5	0.0	5.954840868787463E-5	0.0	1.786452260636239E-4	0.0
6	0.0	5.954840868787463E-5	0.0	1.786452260636239E-4	0.0
7	0.0	5.954840868787463E-5	0.0	1.786452260636239E-4	0.0
8	0.0	5.954840868787463E-5	0.0	1.786452260636239E-4	0.0
9	0.0	5.954840868787463E-5	0.0	2.977420434393732E-4	0.0
10	0.0	5.954840868787463E-5	0.0	4.7638726950299706E-4	0.0
11	0.0	5.954840868787463E-5	0.0	5.954840868787464E-4	0.0
12	0.0	5.954840868787463E-5	0.0	0.001131419765069618	0.0
13	0.0	1.1909681737574926E-4	0.0	0.001131419765069618	0.0
14	0.0	1.1909681737574926E-4	0.0	0.001131419765069618	0.0
15	0.0	1.1909681737574926E-4	0.0	0.001131419765069618	0.0
16	0.0	1.1909681737574926E-4	0.0	0.001131419765069618	0.0
17	0.0	1.1909681737574926E-4	0.0	0.0012505165824453672	0.0
18	0.0	1.1909681737574926E-4	0.0	0.0012505165824453672	0.0
19	0.0	1.1909681737574926E-4	0.0	0.0015482586258847406	0.0
20	0.0	1.1909681737574926E-4	0.0	0.0018460006693241137	0.0
21	0.0	1.1909681737574926E-4	0.0	0.002084194304075612	0.0
22	0.0	1.1909681737574926E-4	0.0	0.0023223879388271106	0.0
23	0.0	1.1909681737574926E-4	0.0	0.0033347108865209793	0.0
24	0.0	1.1909681737574926E-4	0.0	0.006193034503538962	0.0
25	0.0	1.1909681737574926E-4	0.0	0.010837810381193183	0.0
26	0.0	1.786452260636239E-4	0.0	0.02006781372781375	0.0
27	0.0	1.786452260636239E-4	0.0	0.07014802543431632	0.0
28	0.0	2.3819363475149853E-4	0.0	0.1555999919014164	0.0
29	0.0	2.977420434393732E-4	0.0	0.259809707105197	0.0
30	0.0	2.977420434393732E-4	0.0	0.3699147147690772	0.0
31	0.0	2.977420434393732E-4	0.0	0.5766667897333779	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1595	0.0	26.09718	1
CGTTATG	45	0.003826532	20.555555	12
CGCGAAT	125	8.58563E-9	17.76	28
GTATCAA	2455	0.0	16.955194	2
TTGCGCA	80	3.3837266E-4	16.1875	9
AGACCGT	115	1.2429537E-6	16.086956	6
GGCTACG	255	0.0	15.960786	1
TCACGCT	70	0.002593161	15.857142	4
GCTACGA	260	0.0	15.653846	2
TACGCAT	75	0.0041054534	14.8	28
ACCGTCT	115	2.2109334E-5	14.478261	8
GTATTAG	355	0.0	14.070422	1
TTAGGCA	395	0.0	14.050634	4
TACGAGC	290	0.0	14.034483	4
GCGTCTC	265	0.0	13.962264	9
CGAATTG	160	1.7846469E-7	13.875001	30
ATACGCC	80	0.006300215	13.875001	6
CGAGCGT	255	1.8189894E-12	13.784315	6
CGCCGTA	95	0.0012454925	13.6315775	25
GACTGCG	95	0.0012454925	13.6315775	7
>>END_MODULE