FastQCFastQC Report
Fri 10 Feb 2017
ERR1630084.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630084.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477125
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21770.4562745611736967No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19130.40094314906995016No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15080.3160597327744302No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11030.23117631647891013No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5700.11946554886036155No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5470.11464500916950485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4900.030.9591851
TTGCGCA250.005493161329.69
CGAGTTC358.861762E-426.4285714
TTATACG551.8996354E-523.54545435
GTGGTAT652.6773869E-622.769231
ATACGAA502.6988934E-422.237
CGGTGTA450.003822577220.5555579
ACTCGTA450.003822577220.55555715
CGCAGAT450.003822577220.55555719
TATACGA555.1370345E-420.18181836
TGGTATC656.89132E-519.9230772
GTATCAA8000.018.9625022
CGAACTA500.007028999318.524
ATTACAC500.007028999318.53
GCACCGC701.2173015E-418.510
TTGCCGA500.007028999318.510
CGTTTGG609.226023E-418.528
TAGTCGG500.007028999318.55
TGCCGAG500.007028999318.511
TACGCCA852.7177126E-517.41176415
Produced by FastQC (version 0.11.5)