##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630084.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	477125
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.76905842284517	34.0	31.0	34.0	31.0	34.0
2	33.00548703170029	34.0	33.0	34.0	31.0	34.0
3	33.132749279538906	34.0	33.0	34.0	31.0	34.0
4	36.421749017553054	37.0	37.0	37.0	35.0	37.0
5	36.380929525805605	37.0	37.0	37.0	35.0	37.0
6	36.444529211422584	37.0	37.0	37.0	35.0	37.0
7	36.36846738276133	37.0	37.0	37.0	35.0	37.0
8	36.384509300497776	37.0	37.0	37.0	35.0	37.0
9	38.15822059208803	39.0	39.0	39.0	37.0	39.0
10	38.12160125753209	39.0	39.0	39.0	37.0	39.0
11	38.167844904375166	39.0	39.0	39.0	37.0	39.0
12	38.100927429918784	39.0	39.0	39.0	37.0	39.0
13	38.126239455069424	39.0	39.0	39.0	37.0	39.0
14	39.54964631909877	41.0	39.0	41.0	37.0	41.0
15	39.539567199371234	41.0	39.0	41.0	37.0	41.0
16	39.44803353418915	41.0	39.0	41.0	37.0	41.0
17	39.38225412627718	41.0	39.0	41.0	36.0	41.0
18	39.29620539690857	41.0	39.0	41.0	36.0	41.0
19	39.32855960178151	41.0	39.0	41.0	36.0	41.0
20	39.53511763164789	41.0	39.0	41.0	37.0	41.0
21	39.546741419963325	41.0	39.0	41.0	37.0	41.0
22	39.54767618548598	41.0	39.0	41.0	37.0	41.0
23	39.48452501964894	41.0	39.0	41.0	37.0	41.0
24	39.46273408435945	41.0	39.0	41.0	37.0	41.0
25	39.43499083049515	41.0	39.0	41.0	36.0	41.0
26	39.27732564841499	41.0	39.0	41.0	36.0	41.0
27	39.18884569033272	41.0	39.0	41.0	36.0	41.0
28	39.14803248624574	41.0	39.0	41.0	35.0	41.0
29	39.04766884988211	41.0	39.0	41.0	35.0	41.0
30	38.94939271679329	40.0	39.0	41.0	35.0	41.0
31	38.80902488865601	40.0	39.0	41.0	35.0	41.0
32	38.71872779669898	40.0	38.0	41.0	35.0	41.0
33	38.63259523185748	40.0	38.0	41.0	35.0	41.0
34	38.5687587110296	40.0	38.0	41.0	35.0	41.0
35	38.44036678019387	40.0	38.0	41.0	35.0	41.0
36	38.436045061566674	40.0	38.0	41.0	35.0	41.0
37	38.386022530783336	40.0	38.0	41.0	35.0	41.0
38	38.30046843070474	40.0	38.0	41.0	34.0	41.0
39	38.257406340057635	40.0	38.0	41.0	34.0	41.0
40	38.18444642389311	40.0	38.0	41.0	34.0	41.0
41	38.085589730154574	40.0	38.0	41.0	34.0	41.0
42	37.98701178936337	40.0	38.0	41.0	34.0	41.0
43	37.69390620906471	40.0	38.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	1.0
14	0.0
15	1.0
16	2.0
17	3.0
18	11.0
19	32.0
20	47.0
21	95.0
22	151.0
23	303.0
24	462.0
25	716.0
26	1067.0
27	1494.0
28	2093.0
29	2727.0
30	3716.0
31	4752.0
32	6190.0
33	8041.0
34	11607.0
35	16260.0
36	24365.0
37	44378.0
38	92476.0
39	256133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.50248886560126	20.05742729892586	12.671312549122346	24.76877128635054
2	18.89672517684045	21.665601257532092	35.37353942887084	24.064134136756614
3	18.367094576892846	24.693738538118943	31.09059470788577	25.848572177102437
4	13.412208540738801	16.65852763950747	38.061304689546766	31.86795913020697
5	13.091747445637935	37.780455855383806	36.01152737752161	13.116269321456642
6	31.53219806130469	38.20948388787006	15.836311239193085	14.422006811632171
7	27.542048729368613	30.99418391406864	23.687084097458737	17.776683259104008
8	25.647786219544145	34.691328268273516	20.537804558553837	19.123080953628506
9	25.370919570343204	14.949960702122086	21.25124443280063	38.42787529473408
10	16.270159811370185	27.39491747445638	35.34189153785696	20.993031176316478
11	33.252921142258316	23.46366256222164	23.029394812680117	20.254021482839928
12	19.901912496725178	27.352790149331934	30.876395074665968	21.86890227927692
13	28.88865601257532	21.454755043227667	25.904113177888394	23.75247576630862
14	21.189834948912758	22.172386691118678	27.531778883940266	29.105999476028295
15	23.586691118679592	29.268011527377524	24.183390096934765	22.96190725700812
16	22.661357086717317	27.495520041917736	26.56012575320933	23.282997118155617
17	21.754047681425202	28.166832591040087	27.492585800366783	22.58653392716793
18	22.0675923500131	27.012208540738804	28.0356300759759	22.884569033272204
19	22.92564841498559	26.294367304165572	29.30720461095101	21.472779669897825
20	22.79758973015457	26.98014147236049	28.456274561173693	21.76599423631124
21	21.966989782551742	27.13670421797223	28.478700550170288	22.41760544930574
22	22.376735656274562	26.833219806130472	28.60298663872151	22.187057898873462
23	22.20885512182342	26.985800366780193	28.58454283468693	22.22080167670946
24	21.828661252292374	27.55944458999214	27.910086455331413	22.701807702384073
25	22.773487031700288	26.573749017553048	28.671731726486772	21.981032224259888
26	21.954833638983494	27.171915116583705	28.434267749541526	22.438983494891275
27	21.777940791197274	27.522137804558554	27.98700550170291	22.712915902541265
28	21.98669111867959	27.17652606759235	28.95362850406078	21.883154309667276
29	22.298349489127585	27.00424417081478	28.91129159025413	21.786114749803513
30	21.986900707361805	27.365155881582393	28.372858265653655	22.275085145402148
31	22.28074403982185	27.061252292376214	28.71050563269583	21.947498035106104
32	21.709614880796437	26.992716793293166	28.662509824469478	22.635158501440923
33	21.431700288184437	27.04846738276133	28.899135446685882	22.620696882368353
34	22.07157453497511	25.902855645795125	29.0328530259366	22.992716793293162
35	21.933036416033534	26.72171862719413	29.08587896253602	22.259365994236312
36	22.217238669111868	26.548807964369924	28.318784385643177	22.915168980875034
37	21.832224259889966	25.409693476552263	29.528530259365994	23.229552004191774
38	21.461461881058423	25.908304951532617	29.689494367304164	22.940738800104796
39	20.73838092742992	25.93597065758449	30.39496987162693	22.93067854335866
40	21.026984542834686	25.29693476552266	30.82462667015981	22.85145402148284
41	20.76960964107938	25.272203301021744	30.6347393240765	23.323447733822373
42	20.01446161907257	25.82006811632172	30.55488603615405	23.610584228451664
43	20.245009169504847	25.1192035630076	31.166256222164	23.469531045323553
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	624.0
1	521.0
2	418.0
3	708.0
4	998.0
5	998.0
6	1095.5
7	1193.0
8	1004.5
9	816.0
10	1093.5
11	1371.0
12	1371.0
13	2209.5
14	3048.0
15	3709.5
16	4371.0
17	4374.5
18	4378.0
19	4378.0
20	5286.0
21	6194.0
22	5868.5
23	5543.0
24	6421.5
25	7300.0
26	7300.0
27	8404.0
28	9508.0
29	11475.0
30	13442.0
31	15477.0
32	17512.0
33	17512.0
34	19913.5
35	22315.0
36	24508.5
37	26702.0
38	29008.5
39	31315.0
40	31315.0
41	33771.0
42	36227.0
43	37260.0
44	38293.0
45	37522.5
46	36752.0
47	36752.0
48	35928.0
49	35104.0
50	34571.5
51	34039.0
52	31916.0
53	29793.0
54	29793.0
55	28168.5
56	26544.0
57	23833.0
58	21122.0
59	19594.5
60	18067.0
61	18067.0
62	16189.0
63	14311.0
64	12304.0
65	10297.0
66	8947.0
67	7597.0
68	7597.0
69	6165.0
70	4733.0
71	3922.5
72	3112.0
73	2447.5
74	1783.0
75	1783.0
76	1413.5
77	1044.0
78	811.5
79	579.0
80	457.5
81	336.0
82	336.0
83	256.5
84	177.0
85	129.0
86	81.0
87	62.5
88	44.0
89	44.0
90	34.0
91	24.0
92	17.0
93	10.0
94	8.0
95	6.0
96	6.0
97	3.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	477125.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.68258065327823
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.3733452598845	54.76786761199436
2	6.8233250965807235	8.554072513799174
3	1.899132438234006	3.5712756669258003
4	0.892937510800711	2.2388651015641226
5	0.5439880673881758	1.7049287954240142
6	0.3703348779756073	1.3928127514456774
7	0.30664117138907704	1.3454741970048072
8	0.21285811444487052	1.0673996741116258
9	0.17276859288805513	0.9746623132253015
>10	1.2651416696497202	15.653445861516907
>50	0.11114878711181148	4.72152891366468
>100	0.02635138410620418	2.352082056469543
>500	6.756765155436969E-4	0.23654232979890877
>1k	0.0013513530310873937	1.419042213055047
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2177	0.4562745611736967	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1913	0.40094314906995016	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1508	0.3160597327744302	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1103	0.23117631647891013	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	570	0.11946554886036155	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	547	0.11464500916950485	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.191773644223212E-4	0.0
10	0.0	0.0	0.0	8.383547288446424E-4	0.0
11	0.0	0.0	0.0	8.383547288446424E-4	0.0
12	0.0	0.0	0.0	8.383547288446424E-4	0.0
13	0.0	0.0	0.0	8.383547288446424E-4	0.0
14	0.0	0.0	0.0	8.383547288446424E-4	0.0
15	0.0	0.0	0.0	8.383547288446424E-4	0.0
16	0.0	0.0	0.0	8.383547288446424E-4	0.0
17	0.0	0.0	0.0	0.001047943411055803	0.0
18	0.0	0.0	0.0	0.0012575320932669636	0.0
19	0.0	0.0	0.0	0.0018862981399004454	0.0
20	0.0	0.0	0.0	0.0023054755043227667	0.0
21	0.0	0.0	0.0	0.002515064186533927	0.0
22	0.0	0.0	0.0	0.0035630075975897303	0.0
23	0.0	0.0	0.0	0.0052397170552790145	0.0
24	0.0	0.0	0.0	0.008593135970657584	0.0
25	0.0	0.0	0.0	0.0138328530259366	0.0
26	0.0	0.0	0.0	0.025569819229761592	0.0
27	0.0	0.0	0.0	0.08697930311763165	0.0
28	0.0	0.0	0.0	0.1984804820539691	0.0
29	0.0	0.0	0.0	0.322347393240765	0.0
30	0.0	0.0	0.0	0.46780193869531045	0.0
31	0.0	0.0	0.0	0.7809274299187844	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	490	0.0	30.959185	1
TTGCGCA	25	0.0054931613	29.6	9
CGAGTTC	35	8.861762E-4	26.42857	14
TTATACG	55	1.8996354E-5	23.545454	35
GTGGTAT	65	2.6773869E-6	22.76923	1
ATACGAA	50	2.6988934E-4	22.2	37
CGGTGTA	45	0.0038225772	20.555557	9
ACTCGTA	45	0.0038225772	20.555557	15
CGCAGAT	45	0.0038225772	20.555557	19
TATACGA	55	5.1370345E-4	20.181818	36
TGGTATC	65	6.89132E-5	19.923077	2
GTATCAA	800	0.0	18.962502	2
CGAACTA	50	0.0070289993	18.5	24
ATTACAC	50	0.0070289993	18.5	3
GCACCGC	70	1.2173015E-4	18.5	10
TTGCCGA	50	0.0070289993	18.5	10
CGTTTGG	60	9.226023E-4	18.5	28
TAGTCGG	50	0.0070289993	18.5	5
TGCCGAG	50	0.0070289993	18.5	11
TACGCCA	85	2.7177126E-5	17.411764	15
>>END_MODULE