FastQCFastQC Report
Fri 10 Feb 2017
ERR1630083.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630083.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1532111
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT45850.2992603016361086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37600.24541302816832464No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30400.19841904405098584No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21750.14196099368779416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10950.028.0456641
CGAGAAT1052.260822E-819.38095314
CGTGCGA801.6172435E-518.510
CCTCGCG852.7240741E-517.4117649
GTATCAA17600.017.343752
TCTACGC650.001580259117.07692310
TTCGCGG650.001580259117.07692331
TTCGATA650.001580259117.07692332
TCGCGGA803.3834856E-416.187532
ATCCGTA700.00259302215.85714212
CGCGCTT855.365599E-415.23529412
TACGCAT1001.0937417E-414.79999928
GTATTAG2650.014.66037751
GGTGCGT908.2762307E-414.3888898
TGCGTTA1551.2107921E-714.32258137
GTTCTAC2900.014.0344831
CCTAGAC1601.7843013E-713.8753
GTCTTCG800.006299884513.8759
AGTCCGT1356.5699114E-613.7037046
CCGTAGA950.00124540513.6315791
Produced by FastQC (version 0.11.5)