FastQCFastQC Report
Fri 10 Feb 2017
ERR1630082.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630082.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4022153
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT109600.2724908773982492No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT93810.233233295700089No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT82010.20389577422837965No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59980.14912411337907833No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG44640.1109853354658562No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC42590.10588856266780503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24450.028.2985691
GCGTAAT701.22015874E-418.51
CGACGTT1653.6379788E-1217.93939424
GTATCAA39250.017.6280252
CGCGAAT803.3851966E-416.187528
CGTGCGA1504.6820787E-916.03333310
CGAACTA2500.014.80000124
ACGTTAC1752.242814E-914.79999926
GAGTACG40800.013.9203431
GACGTTA2009.858923E-1013.87499925
GACAGGC16500.013.4545467
TCTATAC5200.013.1634633
CCGCCTA2254.0745363E-1013.15555615
CGAGCGT3100.013.1290326
ATTCGCG850.00941007713.05882330
TACGCTA850.00941007713.0588235
AGTACGG43150.012.9049832
ATCCGCG8100.012.7901237
TACGAGC3800.012.6578954
TATACTG5200.012.4519245
Produced by FastQC (version 0.11.5)