##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630082.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4022153
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.824596428828045	34.0	31.0	34.0	31.0	34.0
2	33.05608886583877	34.0	33.0	34.0	31.0	34.0
3	33.18081510076817	34.0	33.0	34.0	31.0	34.0
4	36.4696765637707	37.0	37.0	37.0	35.0	37.0
5	36.424879908844844	37.0	37.0	37.0	35.0	37.0
6	36.46719978081391	37.0	37.0	37.0	35.0	37.0
7	36.397423220847145	37.0	37.0	37.0	35.0	37.0
8	36.40115878237352	37.0	37.0	37.0	35.0	37.0
9	38.18161417529368	39.0	39.0	39.0	37.0	39.0
10	38.15565096603734	39.0	39.0	39.0	37.0	39.0
11	38.19047609576264	39.0	39.0	39.0	37.0	39.0
12	38.115566464030586	39.0	39.0	39.0	37.0	39.0
13	38.133123976139146	39.0	39.0	39.0	37.0	39.0
14	39.59578091634008	41.0	40.0	41.0	37.0	41.0
15	39.58446782108985	41.0	40.0	41.0	37.0	41.0
16	39.50563814951843	41.0	39.0	41.0	37.0	41.0
17	39.41634716531171	41.0	39.0	41.0	37.0	41.0
18	39.29248191205058	41.0	39.0	41.0	36.0	41.0
19	39.32736870029559	41.0	39.0	41.0	36.0	41.0
20	39.548014210299804	41.0	39.0	41.0	37.0	41.0
21	39.56521818041233	41.0	40.0	41.0	37.0	41.0
22	39.58321227462009	41.0	40.0	41.0	37.0	41.0
23	39.5234552241051	41.0	40.0	41.0	37.0	41.0
24	39.48378343638345	41.0	40.0	41.0	37.0	41.0
25	39.4562640953738	41.0	40.0	41.0	37.0	41.0
26	39.309832321147404	41.0	39.0	41.0	36.0	41.0
27	39.22944925267637	41.0	39.0	41.0	36.0	41.0
28	39.185131694393526	41.0	39.0	41.0	36.0	41.0
29	39.08601736433199	41.0	39.0	41.0	36.0	41.0
30	39.00120507598791	40.0	39.0	41.0	35.0	41.0
31	38.87302049424773	40.0	39.0	41.0	35.0	41.0
32	38.78465712269026	40.0	39.0	41.0	35.0	41.0
33	38.716275089485656	40.0	38.0	41.0	35.0	41.0
34	38.62969857188426	40.0	38.0	41.0	35.0	41.0
35	38.511365679028124	40.0	38.0	41.0	35.0	41.0
36	38.49702758696648	40.0	38.0	41.0	35.0	41.0
37	38.439711020441045	40.0	38.0	41.0	35.0	41.0
38	38.34933728279357	40.0	38.0	41.0	34.0	41.0
39	38.28757061205777	40.0	38.0	41.0	34.0	41.0
40	38.22511078022144	40.0	38.0	41.0	34.0	41.0
41	38.12010482942842	40.0	38.0	41.0	34.0	41.0
42	38.03355665485624	40.0	38.0	41.0	34.0	41.0
43	37.734030505552624	40.0	38.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	8.0
14	9.0
15	11.0
16	24.0
17	67.0
18	131.0
19	230.0
20	488.0
21	924.0
22	1666.0
23	2723.0
24	4491.0
25	6550.0
26	9411.0
27	13218.0
28	18163.0
29	23685.0
30	31263.0
31	39963.0
32	51321.0
33	67007.0
34	91462.0
35	128795.0
36	193459.0
37	344031.0
38	741648.0
39	2251403.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.17497295602629	18.706647907227797	11.586033649142635	25.53234548760328
2	18.977373560876476	20.876654866187337	35.14806622224466	24.997905350691532
3	19.118864946211644	22.796074639627083	30.433849731723285	27.651210682437988
4	13.940593508004294	15.641274710335484	35.6915313763549	34.72660040530532
5	14.468420271431743	36.394289327134	34.789253417261854	14.348036984172408
6	34.54095356392459	35.64837041256263	15.38422332516938	14.4264526983434
7	29.37404419971095	30.208870721725408	21.824306534336213	18.592778544227432
8	26.507072207347658	34.33484504443267	20.121014789840167	19.037067958379506
9	25.9739497726715	14.17402570215504	19.874281261801826	39.977743263371636
10	16.25890412423396	26.86660601921409	34.44975862429898	22.424731232252977
11	35.01390921727741	22.220686284186602	21.92999619855336	20.83540829998262
12	20.81887486627187	25.18807713182467	30.57566432704077	23.41738367486269
13	29.786808209434103	20.1800876296849	25.30719244146108	24.725911719419923
14	21.928753083236764	21.11587997771343	26.13274532321371	30.822621615836095
15	24.252433957634135	28.246936404458012	23.343840972732764	24.156788665175092
16	24.274760308720232	26.90312874721573	24.39014627240684	24.431964671657195
17	23.287130051989568	26.807135382468044	26.108778059909703	23.79695650563268
18	22.82889288398527	25.7409402377284	27.236731173577933	24.1934357047084
19	24.67265665925687	25.61909007439548	27.11169863503452	22.59655463131313
20	24.37055477501726	25.813761932974703	26.880827258435968	22.93485603357207
21	23.699744887874726	25.904981734906652	26.437432887311846	23.957840489906772
22	24.207134835497307	25.600990315385815	26.616043696000624	23.57583115311625
23	23.203667289633188	25.99841428210215	26.681431561653675	24.11648686661099
24	23.836164362718176	26.095327552184116	26.47030085628269	23.598207228815017
25	24.021637167954577	25.606584334310504	26.80412704340188	23.567651454333042
26	24.412348311961278	25.83327884344529	26.258449144028084	23.495923700565342
27	24.053734405429132	25.90826355934247	25.917089678090317	24.12091235713808
28	23.543957676398687	25.829822982865146	26.80937299003792	23.816846350698246
29	23.709242288893538	26.100573498820157	27.103767559314623	23.086416652971682
30	23.646116893116698	25.96848006527847	26.38082141579398	24.004581625810854
31	23.390830731700163	25.503082553050564	27.06202374698327	24.044062968266
32	23.012948537760746	25.952145530018377	27.036813368362665	23.99809256385821
33	23.40440555095741	25.599995823132538	27.108292499067044	23.88730612684301
34	24.061143372716053	25.380983766654325	26.9669751498762	23.59089771075342
35	23.922685188753388	25.40820799208782	26.841718850575795	23.827387968582993
36	23.637489672819505	25.34428700250836	26.362547620639994	24.65567570403214
37	23.201280508225324	24.6389930964834	27.851924081455877	24.3078023138354
38	23.234894346386127	24.61256446485253	27.79695352215592	24.355587666605423
39	22.413816679773248	25.154115221375218	28.527333495269826	23.904734603581716
40	23.025131067863406	24.064226298701218	28.7643707238387	24.146271909596674
41	22.050379485812698	24.47865608294861	28.979553985141788	24.491410446096904
42	21.85247552741032	24.898704748427026	28.559107522762062	24.689712201400592
43	22.143588272251204	23.898146092403742	28.94591528467465	25.0123503506704
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2415.0
1	2066.0
2	1717.0
3	3472.0
4	5227.0
5	5227.0
6	5872.5
7	6518.0
8	5598.5
9	4679.0
10	6179.5
11	7680.0
12	7680.0
13	12259.0
14	16838.0
15	20968.5
16	25099.0
17	26989.5
18	28880.0
19	28880.0
20	35719.0
21	42558.0
22	45132.0
23	47706.0
24	55450.5
25	63195.0
26	63195.0
27	71369.0
28	79543.0
29	92950.5
30	106358.0
31	119111.0
32	131864.0
33	131864.0
34	152171.5
35	172479.0
36	185701.5
37	198924.0
38	205180.5
39	211437.0
40	211437.0
41	223195.5
42	234954.0
43	241570.5
44	248187.0
45	247963.0
46	247739.0
47	247739.0
48	248502.0
49	249265.0
50	260551.5
51	271838.0
52	284852.5
53	297867.0
54	297867.0
55	287199.0
56	276531.0
57	251001.5
58	225472.0
59	210659.5
60	195847.0
61	195847.0
62	185589.0
63	175331.0
64	148135.0
65	120939.0
66	106431.5
67	91924.0
68	91924.0
69	79540.5
70	67157.0
71	63782.5
72	60408.0
73	56515.5
74	52623.0
75	52623.0
76	39693.5
77	26764.0
78	20635.5
79	14507.0
80	10392.0
81	6277.0
82	6277.0
83	4502.5
84	2728.0
85	2067.5
86	1407.0
87	1045.5
88	684.0
89	684.0
90	524.5
91	365.0
92	247.0
93	129.0
94	92.0
95	55.0
96	55.0
97	37.5
98	20.0
99	19.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4022153.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.37491496425483
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.54286057643974	39.76796891939544
2	11.136906549876903	10.997676277300496
3	3.3556498784680353	4.9705478219751384
4	1.4993485959383532	2.9612083770492426
5	0.8318589802828621	2.0536483206859026
6	0.5108829201146998	1.5134880442412095
7	0.34974968172106935	1.2088202555627094
8	0.24011846289861183	0.948466294957323
9	0.17889581752821224	0.7949669200124696
>10	1.0886113096879328	10.302396783263838
>50	0.13256962328123645	4.589606330384382
>100	0.11609451967800075	11.19619290168809
>500	0.011171847234549772	3.7325124896202757
>1k	0.0050781123556678815	4.09647412659724
>5k	1.5234337067003643E-4	0.5912245604149988
>10k+	5.078112355667881E-5	0.27480157685107665
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10960	0.2724908773982492	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9381	0.233233295700089	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8201	0.20389577422837965	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5998	0.14912411337907833	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	4464	0.1109853354658562	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	4259	0.10588856266780503	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4862306331957037E-5	0.0	0.0	0.0	0.0
2	2.4862306331957037E-5	2.4862306331957037E-5	0.0	0.0	0.0
3	2.4862306331957037E-5	2.4862306331957037E-5	0.0	2.4862306331957037E-5	0.0
4	2.4862306331957037E-5	2.4862306331957037E-5	0.0	7.458691899587112E-5	0.0
5	7.458691899587112E-5	1.4917383799174224E-4	0.0	7.458691899587112E-5	0.0
6	7.458691899587112E-5	1.4917383799174224E-4	0.0	7.458691899587112E-5	4.9724612663914075E-5
7	7.458691899587112E-5	1.4917383799174224E-4	0.0	9.944922532782815E-5	4.9724612663914075E-5
8	9.944922532782815E-5	1.4917383799174224E-4	0.0	9.944922532782815E-5	4.9724612663914075E-5
9	1.243115316597852E-4	1.4917383799174224E-4	0.0	2.983476759834845E-4	4.9724612663914075E-5
10	1.7403614432369928E-4	1.4917383799174224E-4	0.0	5.718330456350119E-4	4.9724612663914075E-5
11	2.2376075698761334E-4	2.2376075698761334E-4	0.0	6.21557658298926E-4	4.9724612663914075E-5
12	2.7348536965152745E-4	2.2376075698761334E-4	0.0	7.707314962906683E-4	4.9724612663914075E-5
13	3.4807228864739857E-4	2.2376075698761334E-4	0.0	7.707314962906683E-4	4.9724612663914075E-5
14	3.4807228864739857E-4	2.2376075698761334E-4	0.0	7.707314962906683E-4	4.9724612663914075E-5
15	3.977969013113126E-4	2.2376075698761334E-4	0.0	8.204561089545822E-4	4.9724612663914075E-5
16	3.977969013113126E-4	2.2376075698761334E-4	0.0	8.701807216184963E-4	4.9724612663914075E-5
17	4.475215139752267E-4	2.2376075698761334E-4	0.0	9.447676406143674E-4	4.9724612663914075E-5
18	4.475215139752267E-4	2.983476759834845E-4	0.0	9.447676406143674E-4	4.9724612663914075E-5
19	4.723838203071837E-4	2.983476759834845E-4	0.0	0.0011436660912700239	4.9724612663914075E-5
20	4.972461266391408E-4	2.983476759834845E-4	0.0	0.0015414629925813365	9.944922532782815E-5
21	5.469707393030549E-4	2.983476759834845E-4	0.0	0.0016657745242411215	9.944922532782815E-5
22	5.718330456350119E-4	2.983476759834845E-4	0.0	0.0022873321825400477	9.944922532782815E-5
23	5.718330456350119E-4	3.729345949793556E-4	0.0	0.003828795175121384	1.243115316597852E-4
24	5.718330456350119E-4	3.729345949793556E-4	0.0	0.006812271934956229	1.243115316597852E-4
25	5.718330456350119E-4	3.729345949793556E-4	0.0	0.011063726317720882	1.4917383799174224E-4
26	5.718330456350119E-4	3.729345949793556E-4	0.0	0.02056112733652847	1.4917383799174224E-4
27	6.21557658298926E-4	3.729345949793556E-4	0.0	0.07125536994738887	1.4917383799174224E-4
28	6.961445772947971E-4	4.2265920764326964E-4	0.0	0.17833732331912783	1.4917383799174224E-4
29	6.961445772947971E-4	4.2265920764326964E-4	0.0	0.29516530077299397	1.4917383799174224E-4
30	7.458691899587112E-4	4.2265920764326964E-4	0.0	0.41773647098954214	1.4917383799174224E-4
31	7.458691899587112E-4	4.2265920764326964E-4	0.0	0.6658374258761415	1.4917383799174224E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2445	0.0	28.298569	1
GCGTAAT	70	1.22015874E-4	18.5	1
CGACGTT	165	3.6379788E-12	17.939394	24
GTATCAA	3925	0.0	17.628025	2
CGCGAAT	80	3.3851966E-4	16.1875	28
CGTGCGA	150	4.6820787E-9	16.033333	10
CGAACTA	250	0.0	14.800001	24
ACGTTAC	175	2.242814E-9	14.799999	26
GAGTACG	4080	0.0	13.920343	1
GACGTTA	200	9.858923E-10	13.874999	25
GACAGGC	1650	0.0	13.454546	7
TCTATAC	520	0.0	13.163463	3
CCGCCTA	225	4.0745363E-10	13.155556	15
CGAGCGT	310	0.0	13.129032	6
ATTCGCG	85	0.009410077	13.058823	30
TACGCTA	85	0.009410077	13.058823	5
AGTACGG	4315	0.0	12.904983	2
ATCCGCG	810	0.0	12.790123	7
TACGAGC	380	0.0	12.657895	4
TATACTG	520	0.0	12.451924	5
>>END_MODULE