FastQCFastQC Report
Fri 10 Feb 2017
ERR1630081.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630081.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4753907
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT174990.36809723034127506No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT153260.32238745940970237No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT128740.2708088315568647No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT109950.23128344748856045No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA107740.22663463967637568No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99120.20850218567590828No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53770.11310696654351884No Hit
GTGTTAATCCTGGCTACTGTGTGAGCCTGACCTCAAGCACAGG53320.11216037671750835No Hit
GTGTGAGCCTGACCTCAAGCACAGGATTCCAGAAATGGAAGGA53120.11173967012817036No Hit
GATTAAGGAGAGTGGCACTGATGACTTCAATGTCTGGATTGGC48160.1013061467125882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38700.029.447031
TCTAGAC29900.021.5317733
CTAGACA30050.021.362734
TAGACAT30400.020.8733565
TTCCGAT8250.020.63030418
GTCTAGA28150.020.5701581
CTCTAGT29650.020.4654327
GCAATTA27100.020.34317417
TAGAACT30200.019.97024
GTTCTAG32850.019.9360711
GCCTCTA23300.019.84978525
GCGTTTC8600.019.79069914
CTAGAAC31050.019.4830913
GGCGTTT8750.019.4514313
ACATGTA24750.019.4343438
ACTGATC23150.019.259188
TTCTAGA34550.019.2228662
GACATGT25150.019.1988077
CCTCTAG32000.019.19374826
CAATTAC29500.018.75084718
Produced by FastQC (version 0.11.5)