##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630081.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4753907
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83655612110208	34.0	31.0	34.0	31.0	34.0
2	33.06080346123725	34.0	33.0	34.0	31.0	34.0
3	33.1880987575062	34.0	33.0	34.0	31.0	34.0
4	36.465835995529574	37.0	37.0	37.0	35.0	37.0
5	36.425163554945435	37.0	37.0	37.0	35.0	37.0
6	36.47613152718385	37.0	37.0	37.0	35.0	37.0
7	36.41265068921205	37.0	37.0	37.0	35.0	37.0
8	36.416111842322536	37.0	37.0	37.0	35.0	37.0
9	38.192719798683484	39.0	39.0	39.0	37.0	39.0
10	38.165019425074995	39.0	39.0	39.0	37.0	39.0
11	38.20651329527481	39.0	39.0	39.0	37.0	39.0
12	38.14638022998767	39.0	39.0	39.0	37.0	39.0
13	38.15566270017482	39.0	39.0	39.0	37.0	39.0
14	39.59237759594372	41.0	40.0	41.0	37.0	41.0
15	39.579656480448605	41.0	40.0	41.0	37.0	41.0
16	39.504693928593895	41.0	39.0	41.0	37.0	41.0
17	39.44119584165193	41.0	39.0	41.0	37.0	41.0
18	39.36124392000096	41.0	39.0	41.0	36.0	41.0
19	39.37427993437819	41.0	39.0	41.0	36.0	41.0
20	39.58467235475999	41.0	39.0	41.0	37.0	41.0
21	39.57446369901641	41.0	40.0	41.0	37.0	41.0
22	39.59484630220995	41.0	40.0	41.0	37.0	41.0
23	39.53397805215794	41.0	40.0	41.0	37.0	41.0
24	39.49496571977534	41.0	40.0	41.0	37.0	41.0
25	39.477354310885765	41.0	40.0	41.0	37.0	41.0
26	39.35219157631818	41.0	39.0	41.0	36.0	41.0
27	39.2536351678735	41.0	39.0	41.0	36.0	41.0
28	39.21974851422209	41.0	39.0	41.0	36.0	41.0
29	39.13017860887897	41.0	39.0	41.0	36.0	41.0
30	39.033289250294544	40.0	39.0	41.0	35.0	41.0
31	38.9112578348714	40.0	39.0	41.0	35.0	41.0
32	38.80623432473542	40.0	39.0	41.0	35.0	41.0
33	38.72791663783074	40.0	38.0	41.0	35.0	41.0
34	38.67398836367644	40.0	38.0	41.0	35.0	41.0
35	38.546059903990546	40.0	38.0	41.0	35.0	41.0
36	38.543134310368295	40.0	38.0	41.0	35.0	41.0
37	38.495052806039325	40.0	38.0	41.0	35.0	41.0
38	38.387640944595674	40.0	38.0	41.0	35.0	41.0
39	38.34890186114284	40.0	38.0	41.0	35.0	41.0
40	38.28696101964132	40.0	38.0	41.0	34.0	41.0
41	38.177897884834515	40.0	38.0	41.0	34.0	41.0
42	38.07109373405916	40.0	38.0	41.0	34.0	41.0
43	37.79652988583916	40.0	38.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	0.0
12	0.0
13	7.0
14	9.0
15	10.0
16	35.0
17	56.0
18	117.0
19	207.0
20	426.0
21	861.0
22	1649.0
23	2886.0
24	4748.0
25	7096.0
26	10342.0
27	14806.0
28	20550.0
29	27705.0
30	35722.0
31	46228.0
32	59189.0
33	77708.0
34	105522.0
35	148632.0
36	229079.0
37	409595.0
38	876079.0
39	2674640.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.742271988072126	18.653625323339305	11.217489109484053	25.386613579104512
2	19.210157035045068	21.44267862202605	34.76067159075683	24.586492752172056
3	19.545712610701052	21.97935298271506	30.746436562599982	27.728497843983906
4	14.117125135178284	15.032898203519757	35.50357632153931	35.34640033976264
5	14.45335384137721	36.43746080855178	34.59072716399374	14.518458186077263
6	35.09077901608088	35.57284566147382	14.618460142362903	14.717915180082402
7	29.634109375719802	30.533180392464555	21.10085451818893	18.731855713626707
8	26.333245475773925	34.04709010925119	20.097826903218763	19.52183751175612
9	26.033134430269673	13.65600967793438	19.994185834935347	40.3166700568606
10	15.9699800606112	27.371507267601153	34.17929715495065	22.47921551683699
11	34.88974437236572	21.76632820120377	22.1271472075495	21.216780218881016
12	21.61685115001198	25.78096290062048	30.11350453427044	22.488681415097098
13	30.48130726999918	20.011392734439273	25.58665956233473	23.92064043322682
14	22.497326935507996	20.879710099503416	26.16035610288548	30.462606862103108
15	24.71312543556279	27.79015239465139	23.36539187661854	24.131330293167284
16	23.947144948355113	26.69292857432844	24.91969237092774	24.440234106388704
17	22.885323587524955	26.972509138273004	26.34178581953749	23.800381454664553
18	23.50378330918127	25.17554508323364	27.276764143682236	24.04390746390285
19	24.180447787472495	25.052362193875478	27.367510555002443	23.399679463649584
20	24.108675243331433	25.85940364420255	27.36795229692125	22.66396881554477
21	23.93763697943607	25.27762953713651	27.852374899214478	22.932358584212942
22	23.743312605820854	25.463665990941763	26.92625244877529	23.866768954462085
23	23.518823569750104	25.82532641046617	27.31744647087122	23.338403548912506
24	23.469895393410095	25.673114766443682	26.83704161650617	24.01994822364005
25	24.421470592504228	25.548249050728167	27.134775669780666	22.895504686986932
26	24.494442150424902	25.06980048200354	26.497510363580943	23.93824700399061
27	23.0008916876161	25.43606763868119	27.098090055190394	24.46495061851231
28	23.251422461566875	24.9726593305254	27.76880153524249	24.00711667266524
29	23.40052087682826	25.58602850245072	26.683147146126334	24.330303474594686
30	22.813403796077626	26.04325242374325	27.796420922832528	23.3469228573466
31	24.50125759717218	25.484680285079197	26.90835138339896	23.105710734349664
32	23.47107337186024	25.617371143356397	27.272367759823656	23.6391877249597
33	23.234404880028155	25.272581058064453	27.924021231378738	23.568992830528657
34	23.902676261862084	24.692510812685228	27.416901508590723	23.987911416861962
35	22.65246249032638	25.58787961144381	28.239046325475027	23.520611572754788
36	24.02745783625973	24.20672091397665	28.04909730038892	23.716723949374693
37	23.246731583095755	24.560514120280434	28.13214478112424	24.060609515499568
38	23.07657680303801	24.290862231844248	28.639727281160525	23.992833683957215
39	22.88698537855284	24.52214567933281	29.105512581546083	23.485356360568264
40	22.555132020883033	25.033577644661538	28.677254308929477	23.734036025525953
41	23.270333222757618	23.827580135665254	28.870800375354417	24.03128626622271
42	22.324795163220486	24.289473900099434	28.959611536363667	24.426119400316413
43	21.512621092503494	24.398878648656776	29.37657383705655	24.71192642178318
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4710.0
1	4074.5
2	3439.0
3	6250.5
4	9062.0
5	9062.0
6	9417.5
7	9773.0
8	8234.0
9	6695.0
10	9387.0
11	12079.0
12	12079.0
13	18538.0
14	24997.0
15	30013.0
16	35029.0
17	35184.0
18	35339.0
19	35339.0
20	44500.5
21	53662.0
22	52315.0
23	50968.0
24	59645.0
25	68322.0
26	68322.0
27	77132.0
28	85942.0
29	98677.5
30	111413.0
31	123775.5
32	136138.0
33	136138.0
34	156829.0
35	177520.0
36	198123.5
37	218727.0
38	227471.5
39	236216.0
40	236216.0
41	249829.0
42	263442.0
43	280637.5
44	297833.0
45	316948.5
46	336064.0
47	336064.0
48	334690.5
49	333317.0
50	342928.0
51	352539.0
52	346245.0
53	339951.0
54	339951.0
55	323174.0
56	306397.0
57	292340.5
58	278284.0
59	263449.5
60	248615.0
61	248615.0
62	229864.0
63	211113.0
64	195738.0
65	180363.0
66	157178.5
67	133994.0
68	133994.0
69	109350.0
70	84706.0
71	69583.0
72	54460.0
73	40947.5
74	27435.0
75	27435.0
76	20148.0
77	12861.0
78	9436.0
79	6011.0
80	4534.0
81	3057.0
82	3057.0
83	2368.5
84	1680.0
85	1284.0
86	888.0
87	669.0
88	450.0
89	450.0
90	347.0
91	244.0
92	164.0
93	84.0
94	67.0
95	50.0
96	50.0
97	34.0
98	18.0
99	19.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4753907.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.48944324232827
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.9845071158999	33.98497175366631
2	10.999903850180887	9.34759580626658
3	3.346907832434134	4.266247511505423
4	1.5333986147946366	2.6061301364472604
5	0.8756829800849335	1.8603641140295835
6	0.5557863491470476	1.4169031522166595
7	0.384760901025753	1.1443793526200574
8	0.28852782359916374	0.9807509267719339
9	0.22729667342635007	0.8691938194247033
>10	1.4708457972128504	12.150972728631542
>50	0.16190490241447933	4.802173403093867
>100	0.14081942714180165	12.3255104318199
>500	0.02007139492804471	5.835023536684143
>1k	0.009136978129921645	6.428329018584794
>5k	1.997153689600358E-4	0.5501552935784229
>10k+	2.4964421120004474E-4	1.4312990146588915
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17499	0.36809723034127506	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	15326	0.32238745940970237	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12874	0.2708088315568647	No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT	10995	0.23128344748856045	No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA	10774	0.22663463967637568	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9912	0.20850218567590828	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5377	0.11310696654351884	No Hit
GTGTTAATCCTGGCTACTGTGTGAGCCTGACCTCAAGCACAGG	5332	0.11216037671750835	No Hit
GTGTGAGCCTGACCTCAAGCACAGGATTCCAGAAATGGAAGGA	5312	0.11173967012817036	No Hit
GATTAAGGAGAGTGGCACTGATGACTTCAATGTCTGGATTGGC	4816	0.1013061467125882	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.103532946689954E-5	0.0
5	0.0	0.0	0.0	4.207065893379908E-5	0.0
6	0.0	0.0	0.0	4.207065893379908E-5	2.103532946689954E-5
7	0.0	0.0	0.0	4.207065893379908E-5	2.103532946689954E-5
8	0.0	0.0	0.0	4.207065893379908E-5	4.207065893379908E-5
9	0.0	0.0	0.0	6.310598840069862E-5	4.207065893379908E-5
10	0.0	0.0	0.0	6.310598840069862E-5	4.207065893379908E-5
11	2.103532946689954E-5	0.0	0.0	1.051766473344977E-4	4.207065893379908E-5
12	2.103532946689954E-5	0.0	0.0	1.4724730626829678E-4	4.207065893379908E-5
13	2.103532946689954E-5	0.0	0.0	1.4724730626829678E-4	4.207065893379908E-5
14	4.207065893379908E-5	0.0	0.0	1.8931796520209586E-4	4.207065893379908E-5
15	4.207065893379908E-5	0.0	0.0	2.3138862413589497E-4	4.207065893379908E-5
16	4.207065893379908E-5	0.0	0.0	3.576006009372922E-4	4.207065893379908E-5
17	4.207065893379908E-5	0.0	0.0	4.838125777386895E-4	4.207065893379908E-5
18	4.207065893379908E-5	0.0	0.0	6.100245545400867E-4	4.207065893379908E-5
19	6.310598840069862E-5	0.0	0.0	7.993425197421825E-4	4.207065893379908E-5
20	6.310598840069862E-5	0.0	0.0	0.001093837132278776	4.207065893379908E-5
21	6.310598840069862E-5	0.0	0.0	0.0014935083921498674	6.310598840069862E-5
22	6.310598840069862E-5	2.103532946689954E-5	0.0	0.0019562856404216574	6.310598840069862E-5
23	6.310598840069862E-5	2.103532946689954E-5	0.0	0.0029659814548328354	6.310598840069862E-5
24	6.310598840069862E-5	2.103532946689954E-5	0.0	0.005469185661393881	6.310598840069862E-5
25	6.310598840069862E-5	2.103532946689954E-5	0.0	0.009739357543174489	6.310598840069862E-5
26	6.310598840069862E-5	2.103532946689954E-5	0.0	0.01748035878699352	8.414131786759816E-5
27	6.310598840069862E-5	2.103532946689954E-5	0.0	0.06992143514797408	1.051766473344977E-4
28	6.310598840069862E-5	4.207065893379908E-5	0.0	0.17722265075862864	1.051766473344977E-4
29	6.310598840069862E-5	4.207065893379908E-5	0.0	0.30215147246254503	1.051766473344977E-4
30	6.310598840069862E-5	4.207065893379908E-5	0.0	0.44378234576317965	1.051766473344977E-4
31	6.310598840069862E-5	4.207065893379908E-5	0.0	0.7146122126495112	1.051766473344977E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3870	0.0	29.44703	1
TCTAGAC	2990	0.0	21.531773	3
CTAGACA	3005	0.0	21.36273	4
TAGACAT	3040	0.0	20.873356	5
TTCCGAT	825	0.0	20.630304	18
GTCTAGA	2815	0.0	20.570158	1
CTCTAGT	2965	0.0	20.46543	27
GCAATTA	2710	0.0	20.343174	17
TAGAACT	3020	0.0	19.9702	4
GTTCTAG	3285	0.0	19.936071	1
GCCTCTA	2330	0.0	19.849785	25
GCGTTTC	860	0.0	19.790699	14
CTAGAAC	3105	0.0	19.483091	3
GGCGTTT	875	0.0	19.45143	13
ACATGTA	2475	0.0	19.434343	8
ACTGATC	2315	0.0	19.25918	8
TTCTAGA	3455	0.0	19.222866	2
GACATGT	2515	0.0	19.198807	7
CCTCTAG	3200	0.0	19.193748	26
CAATTAC	2950	0.0	18.750847	18
>>END_MODULE