FastQCFastQC Report
Fri 10 Feb 2017
ERR1630080.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630080.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1413116
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT95600.6765191251107482No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT81140.5741920691578044No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64650.45749959663608647No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47600.336844250578155No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25300.179036965118221No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23920.16927131247540897No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23250.16453001735172484No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16200.11464027015475022No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14900.10544074230282581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18550.032.3126681
GTATCAA30900.019.51782
ACTATAC2050.017.1463413
CGTGCGA1504.6729838E-916.03333310
TAGTCCG1504.6729838E-916.0333335
TACGGGT2800.015.8571434
CTATACT2550.015.2352944
TATTCCG855.3652347E-415.23529327
CATCGTT2251.8189894E-1214.832
TACACCG750.004105025414.85
CGAATTA2059.276846E-1114.43902415
CGAGAAT1702.4374458E-814.147058514
GAGCGTC1051.6560547E-414.0952397
GTGCGAG1854.8985385E-914.011
CCCGCGA800.006299566513.87530
TACGTTA1501.3059871E-613.56666719
AACCACG2051.4242687E-913.5365857
TCTATAC1652.595807E-713.4545453
TATCAAC45750.013.38473
CTACACT2102.040906E-913.2142874
Produced by FastQC (version 0.11.5)