##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630080.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1413116
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.799285408982705	34.0	31.0	34.0	31.0	34.0
2	33.01700355809431	34.0	33.0	34.0	31.0	34.0
3	33.14481967510098	34.0	33.0	34.0	31.0	34.0
4	36.42990667432822	37.0	37.0	37.0	35.0	37.0
5	36.3979001016194	37.0	37.0	37.0	35.0	37.0
6	36.45407454165122	37.0	37.0	37.0	35.0	37.0
7	36.38120578919211	37.0	37.0	37.0	35.0	37.0
8	36.39456138066514	37.0	37.0	37.0	35.0	37.0
9	38.16547473809652	39.0	39.0	39.0	37.0	39.0
10	38.146802527181066	39.0	39.0	39.0	37.0	39.0
11	38.18895759442254	39.0	39.0	39.0	37.0	39.0
12	38.12173664440853	39.0	39.0	39.0	37.0	39.0
13	38.139031756770144	39.0	39.0	39.0	37.0	39.0
14	39.56158730068869	41.0	39.0	41.0	37.0	41.0
15	39.55597488104303	41.0	39.0	41.0	37.0	41.0
16	39.469506395794824	41.0	39.0	41.0	37.0	41.0
17	39.425558835934204	41.0	39.0	41.0	37.0	41.0
18	39.33048949980044	41.0	39.0	41.0	36.0	41.0
19	39.35802439431724	41.0	39.0	41.0	36.0	41.0
20	39.56408744929645	41.0	39.0	41.0	37.0	41.0
21	39.568306494300536	41.0	40.0	41.0	37.0	41.0
22	39.56644252842654	41.0	40.0	41.0	37.0	41.0
23	39.49342304524186	41.0	40.0	41.0	37.0	41.0
24	39.45379855581566	41.0	39.0	41.0	36.0	41.0
25	39.42721404329156	41.0	39.0	41.0	36.0	41.0
26	39.26750316322227	41.0	39.0	41.0	36.0	41.0
27	39.17101709979931	41.0	39.0	41.0	35.0	41.0
28	39.12335293068651	41.0	39.0	41.0	35.0	41.0
29	38.99709577982274	41.0	39.0	41.0	35.0	41.0
30	38.89641614701129	40.0	39.0	41.0	35.0	41.0
31	38.74294679276153	40.0	38.0	41.0	35.0	41.0
32	38.63207125246618	40.0	38.0	41.0	35.0	41.0
33	38.53363276617065	40.0	38.0	41.0	35.0	41.0
34	38.46107609000252	40.0	38.0	41.0	35.0	41.0
35	38.31755850192058	40.0	38.0	41.0	35.0	41.0
36	38.28106751321194	40.0	38.0	41.0	35.0	41.0
37	38.217109564961405	40.0	38.0	41.0	34.0	41.0
38	38.1093002980647	40.0	38.0	41.0	34.0	41.0
39	38.04327033307952	40.0	38.0	41.0	34.0	41.0
40	37.96378782775087	40.0	38.0	41.0	34.0	41.0
41	37.84689650389635	40.0	38.0	41.0	33.0	41.0
42	37.738896877538714	40.0	38.0	41.0	33.0	41.0
43	37.441708960906254	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	2.0
14	5.0
15	5.0
16	11.0
17	21.0
18	37.0
19	91.0
20	122.0
21	318.0
22	514.0
23	943.0
24	1571.0
25	2336.0
26	3439.0
27	4958.0
28	6739.0
29	9159.0
30	11604.0
31	15176.0
32	19399.0
33	25280.0
34	34791.0
35	48557.0
36	75666.0
37	133154.0
38	253059.0
39	766156.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.85352370222969	20.16210983387068	12.909343606611207	24.075022857288435
2	18.59196272634377	22.2741798974748	35.539969825548646	23.593887550632786
3	18.990656110326398	23.956915072789496	31.976426563707438	25.076002253176664
4	13.66830465439497	16.754533951919022	36.87736887842187	32.69979251526414
5	13.600015851494145	37.163898788209885	35.40056159579256	13.83552376450341
6	32.04712139696954	37.08711811344575	15.834581166726583	15.031179322858138
7	26.884558663266144	32.09559583218929	22.727858151772395	18.29198735277217
8	25.781747570616993	34.66403324284772	20.891490861330563	18.662728325204725
9	25.288369815358397	14.720447578259677	21.78313740697862	38.20804519940331
10	16.101862833624416	27.806917478819855	35.03088210734292	21.060337580212806
11	33.0650845365844	22.827849942962928	23.35158614013287	20.755479380319805
12	19.935235323922452	27.061543426017398	31.675318940554064	21.327902309506083
13	29.56268275215906	21.56737309605156	25.848337999145148	23.021606152644228
14	20.736514199825066	22.873847582222552	28.31635902501988	28.0732791929325
15	23.570181075014364	29.22477701759799	24.716513010963006	22.48852889642464
16	22.040724186832502	28.208795314751228	26.795040180707037	22.955440317709233
17	21.410485763376823	27.994021722208227	28.21233359469428	22.383158919720675
18	21.800545744298418	26.591730615179504	29.168447600904667	22.43927603961741
19	22.545282906711126	26.594844301529385	29.750353120338318	21.10951967142117
20	22.682992762094546	26.75449149255971	29.457454306652814	21.105061438692932
21	22.086509529295544	26.89319206632718	29.25952292663872	21.76077547773856
22	21.8631025336915	27.18962915995573	29.39574670444606	21.55152160190671
23	22.184449118119108	26.759515850078834	29.170429037672776	21.885605994129286
24	22.02572187987398	27.04045527755683	29.055293408326	21.87852943424319
25	22.122741515912352	26.76666317556379	29.105466217918412	22.00512909060544
26	21.84286357241727	27.16981479227466	29.055010345930555	21.93231128937752
27	21.573317406355883	27.37765335612929	29.143750406902193	21.90527883061263
28	21.716830040845903	27.2288333017247	29.18493598543927	21.869400671990128
29	21.51698798966256	27.443606894267703	29.43112950387654	21.608275612193196
30	21.67146929197603	27.340076823134122	29.172835068034047	21.8156188168558
31	21.933302007761572	27.379210199304232	29.015240079370695	21.6722477135635
32	21.28339074782254	27.083622292862014	29.520152627243622	22.112834332071817
33	21.149785297173057	26.994316107099486	29.79946444594782	22.056434149779637
34	21.589664259692764	26.377735444223973	29.69395293804613	22.338647358037132
35	21.322665655190374	26.622018291491994	29.860464392165966	22.194851661151667
36	21.590654978076817	26.579841994570863	29.537065605371392	22.292437421980928
37	21.2831076854271	25.791725520056385	30.231629958191686	22.69353683632483
38	20.92305231842255	25.89093888965945	30.854791821761268	22.33121697015673
39	20.603050280373303	25.90077530790112	31.110255633649324	22.385918778076253
40	20.240022758216593	25.535907880174026	31.551054548954227	22.673014812655154
41	20.113069273860038	25.4746956371593	31.685721483586626	22.726513605394036
42	19.691306304648734	25.828099037870917	31.590117159525473	22.890477497954876
43	19.406828597227687	25.55041482794052	31.865890698286627	23.176865876545165
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4295.0
1	3236.5
2	2178.0
3	3350.5
4	4523.0
5	4523.0
6	4883.0
7	5243.0
8	4435.5
9	3628.0
10	4803.0
11	5978.0
12	5978.0
13	9547.5
14	13117.0
15	16132.0
16	19147.0
17	19140.0
18	19133.0
19	19133.0
20	23710.5
21	28288.0
22	26750.0
23	25212.0
24	29381.5
25	33551.0
26	33551.0
27	36754.5
28	39958.0
29	44781.5
30	49605.0
31	54174.0
32	58743.0
33	58743.0
34	63031.5
35	67320.0
36	71781.5
37	76243.0
38	77235.0
39	78227.0
40	78227.0
41	80246.0
42	82265.0
43	82408.5
44	82552.0
45	82444.0
46	82336.0
47	82336.0
48	81463.5
49	80591.0
50	80865.0
51	81139.0
52	80753.5
53	80368.0
54	80368.0
55	79066.5
56	77765.0
57	75740.0
58	73715.0
59	69920.5
60	66126.0
61	66126.0
62	59875.5
63	53625.0
64	46487.0
65	39349.0
66	33753.0
67	28157.0
68	28157.0
69	24043.5
70	19930.0
71	16444.5
72	12959.0
73	10534.0
74	8109.0
75	8109.0
76	6180.5
77	4252.0
78	3311.0
79	2370.0
80	1932.0
81	1494.0
82	1494.0
83	1114.5
84	735.0
85	560.5
86	386.0
87	306.0
88	226.0
89	226.0
90	195.0
91	164.0
92	113.0
93	62.0
94	47.5
95	33.0
96	33.0
97	20.5
98	8.0
99	9.5
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1413116.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.19275303830675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.13931435696183	56.694987317184484
2	10.982643932034732	14.978734507298054
3	2.8698077662819275	5.871002768204346
4	1.0938167985625085	2.9836151525409784
5	0.5340960426813284	1.8210739768652384
6	0.30720869691771596	1.25696440800779
7	0.19375277352470482	0.924877452482002
8	0.14072659553582365	0.7677227180236883
9	0.1087493220832882	0.6674324097518056
>10	0.5705142128086994	7.010391019519156
>50	0.04190189000514141	1.9530625650226465
>100	0.015908051196177924	1.9671033772152635
>500	5.19853802019966E-4	0.22305304810868845
>1k	7.277953228279524E-4	1.168511533973075
>5k	3.119122812119796E-4	1.711467745802807
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9560	0.6765191251107482	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8114	0.5741920691578044	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6465	0.45749959663608647	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4760	0.336844250578155	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2530	0.179036965118221	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2392	0.16927131247540897	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2325	0.16453001735172484	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1620	0.11464027015475022	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1490	0.10544074230282581	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	7.076559886095692E-5	0.0
10	0.0	0.0	0.0	1.4153119772191384E-4	0.0
11	0.0	0.0	0.0	3.538279943047846E-4	0.0
12	0.0	0.0	0.0	4.2459359316574153E-4	0.0
13	0.0	0.0	0.0	4.2459359316574153E-4	0.0
14	0.0	0.0	0.0	4.2459359316574153E-4	0.0
15	0.0	0.0	0.0	4.2459359316574153E-4	0.0
16	0.0	0.0	0.0	4.2459359316574153E-4	0.0
17	0.0	0.0	0.0	6.368903897486123E-4	0.0
18	0.0	0.0	0.0	7.784215874705262E-4	0.0
19	0.0	0.0	0.0	0.0012030151806362676	0.0
20	0.0	0.0	0.0	0.001769139971523923	0.0
21	0.0	0.0	0.0	0.002476795960133492	0.0
22	0.0	0.0	0.0	0.003609045541908803	0.0
23	0.0	0.0	0.0	0.005236654315710812	0.0
24	0.0	0.0	0.0	0.010048715038255883	0.0
25	0.0	0.0	0.0	0.01627608773802009	0.0
26	0.0	0.0	0.0	0.030995332301099132	0.0
27	0.0	0.0	0.0	0.10091174397572457	0.0
28	0.0	0.0	0.0	0.23204039866507775	0.0
29	0.0	0.0	0.0	0.38425720181499606	0.0
30	0.0	0.0	0.0	0.561737323758276	0.0
31	0.0	0.0	0.0	0.9046674158384733	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1855	0.0	32.312668	1
GTATCAA	3090	0.0	19.5178	2
ACTATAC	205	0.0	17.146341	3
CGTGCGA	150	4.6729838E-9	16.033333	10
TAGTCCG	150	4.6729838E-9	16.033333	5
TACGGGT	280	0.0	15.857143	4
CTATACT	255	0.0	15.235294	4
TATTCCG	85	5.3652347E-4	15.235293	27
CATCGTT	225	1.8189894E-12	14.8	32
TACACCG	75	0.0041050254	14.8	5
CGAATTA	205	9.276846E-11	14.439024	15
CGAGAAT	170	2.4374458E-8	14.1470585	14
GAGCGTC	105	1.6560547E-4	14.095239	7
GTGCGAG	185	4.8985385E-9	14.0	11
CCCGCGA	80	0.0062995665	13.875	30
TACGTTA	150	1.3059871E-6	13.566667	19
AACCACG	205	1.4242687E-9	13.536585	7
TCTATAC	165	2.595807E-7	13.454545	3
TATCAAC	4575	0.0	13.3847	3
CTACACT	210	2.040906E-9	13.214287	4
>>END_MODULE