FastQCFastQC Report
Fri 10 Feb 2017
ERR1630079.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630079.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1894918
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT57160.301648936787766No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51570.27214897953367906No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41440.21869020189791855No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30340.16011246924669034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14150.026.5406361
ATTCGCG400.001931646823.12500230
GTATTAG3200.017.9218751
GTATCAA21800.017.2270642
ACGTTAA1751.3278623E-1015.85714320
CGCGCTT700.002593326115.85714212
TCTAGAC1302.5913505E-715.6538463
TTACGCC855.366432E-415.23529434
AACACCG1101.4527783E-515.1363647
ATTTACG1001.09395834E-414.832
TTTACGC750.004105711414.833
TAACGGC1001.09395834E-414.836
TAACGCT1752.240995E-914.832
TTAACGG1001.09395834E-414.835
CGCCGTA750.004105711414.825
TACGTTA1752.240995E-914.819
CTTAACG1651.6467311E-814.57575730
TACCCGT1152.2112295E-514.4782616
TTACGTT1854.9039954E-914.018
TAATTCG2051.4242687E-913.5365865
Produced by FastQC (version 0.11.5)