##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630079.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1894918
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83205869594357	34.0	31.0	34.0	31.0	34.0
2	33.06150292519254	34.0	33.0	34.0	31.0	34.0
3	33.188556444131095	34.0	33.0	34.0	31.0	34.0
4	36.48178338060011	37.0	37.0	37.0	35.0	37.0
5	36.42677466782204	37.0	37.0	37.0	35.0	37.0
6	36.4703876368265	37.0	37.0	37.0	35.0	37.0
7	36.40179311189191	37.0	37.0	37.0	35.0	37.0
8	36.407765401985735	37.0	37.0	37.0	35.0	37.0
9	38.1795502496678	39.0	39.0	39.0	37.0	39.0
10	38.156672742567224	39.0	39.0	39.0	37.0	39.0
11	38.1913919230278	39.0	39.0	39.0	37.0	39.0
12	38.13036500787897	39.0	39.0	39.0	37.0	39.0
13	38.14790560858042	39.0	39.0	39.0	37.0	39.0
14	39.59723534210979	41.0	40.0	41.0	37.0	41.0
15	39.58683436433661	41.0	40.0	41.0	37.0	41.0
16	39.50715439929327	41.0	39.0	41.0	37.0	41.0
17	39.43318549931976	41.0	39.0	41.0	37.0	41.0
18	39.328780453824386	41.0	39.0	41.0	36.0	41.0
19	39.354424307542594	41.0	39.0	41.0	36.0	41.0
20	39.57139728473739	41.0	39.0	41.0	37.0	41.0
21	39.58071800468411	41.0	40.0	41.0	37.0	41.0
22	39.597499733497706	41.0	40.0	41.0	37.0	41.0
23	39.525799533277954	41.0	40.0	41.0	37.0	41.0
24	39.49089775916425	41.0	40.0	41.0	37.0	41.0
25	39.46509664270433	41.0	40.0	41.0	37.0	41.0
26	39.31961541343742	41.0	39.0	41.0	36.0	41.0
27	39.2369590663026	41.0	39.0	41.0	36.0	41.0
28	39.19728716493273	41.0	39.0	41.0	36.0	41.0
29	39.08783440761025	41.0	39.0	41.0	35.0	41.0
30	39.00276054161711	40.0	39.0	41.0	35.0	41.0
31	38.874333876188835	40.0	39.0	41.0	35.0	41.0
32	38.7829520855256	40.0	39.0	41.0	35.0	41.0
33	38.70837366049613	40.0	38.0	41.0	35.0	41.0
34	38.63811310040857	40.0	38.0	41.0	35.0	41.0
35	38.508520157600486	40.0	38.0	41.0	35.0	41.0
36	38.496998814724435	40.0	38.0	41.0	35.0	41.0
37	38.45542867818027	40.0	38.0	41.0	35.0	41.0
38	38.35856960565048	40.0	38.0	41.0	34.0	41.0
39	38.31156915497135	40.0	38.0	41.0	34.0	41.0
40	38.2504446102681	40.0	38.0	41.0	34.0	41.0
41	38.147969463586286	40.0	38.0	41.0	34.0	41.0
42	38.05678504294117	40.0	38.0	41.0	34.0	41.0
43	37.76454653974473	40.0	38.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	3.0
14	2.0
15	9.0
16	11.0
17	35.0
18	62.0
19	109.0
20	241.0
21	412.0
22	739.0
23	1361.0
24	1914.0
25	3016.0
26	4486.0
27	6096.0
28	8239.0
29	11275.0
30	14256.0
31	18755.0
32	24014.0
33	31241.0
34	43093.0
35	60145.0
36	91313.0
37	160755.0
38	349505.0
39	1063829.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.190312192928666	19.382949552434457	12.25905289833122	25.167685356305658
2	18.693157170917157	21.421771285089907	35.269547283840254	24.615524260152682
3	19.042407112075562	23.43478715173955	31.154646269653885	26.368159466531004
4	14.34932804480194	15.970242511813176	35.91828247976957	33.762146963615315
5	14.054434017725306	36.71235377995249	35.210283505671484	14.022928696650727
6	33.62704876939266	36.12837072633222	15.566214474716055	14.678366029559061
7	27.995195570467956	30.969625070847396	22.412632103341675	18.622547255342976
8	26.081656303861166	34.49294375798847	20.450964105043067	18.97443583310729
9	25.58501212189657	14.705438441135712	20.58490129915912	39.124648137808606
10	16.153205574067055	27.319282417497746	34.86615251952855	21.661359488906644
11	34.25900223650839	22.421445149605418	22.66177217167181	20.657780442214385
12	20.21084817390515	25.89040792266473	31.002027528367982	22.89671637506214
13	29.418792792089153	20.84544027762679	25.546646345646618	24.189120584637436
14	21.576923117517484	21.77698454497767	26.47148847601849	30.174603861486354
15	24.020089523662765	28.70873568143846	23.535002570032056	23.73617222486672
16	23.453943653498463	27.65692235759014	25.171907174875113	23.71722681403628
17	22.60915775774994	27.444828747207	26.833034463760434	23.11297903128262
18	22.59337871084659	26.618671625896212	27.52382952718798	23.264120136069213
19	23.66814817316633	26.24509345523131	28.004008616731703	22.082749754870658
20	23.659651763295297	26.196542541682543	27.67760926858049	22.466196426441673
21	22.9353987877048	26.58067525877109	27.163444539552632	23.320481413971475
22	23.24607186168478	26.376866967330514	27.237115273589673	23.13994589739503
23	23.155513853369907	26.496291660114053	27.212628725886816	23.13556576062922
24	23.363491190647828	26.44816292842223	27.17241590401273	23.015929976917207
25	23.608409440408504	26.03088893556344	27.29194614226051	23.06875548176755
26	23.199631857420744	26.47829615846174	27.259913093864746	23.06215889025277
27	23.023476477610114	26.664689448303303	26.994466251310083	23.3173678227765
28	22.989543610858096	26.513495570784595	27.366619558207795	23.130341260149514
29	23.053398616721147	26.685429132025767	27.46688774923242	22.794284502020666
30	22.95571628957031	26.976629067854123	27.249516865637457	22.818137776938105
31	23.3196898229897	26.41702701647248	27.37928501391617	22.883998146621646
32	22.727474223158996	26.5696985304905	27.607843716720197	23.094983529630305
33	22.7778721823319	26.403675515246572	27.571694395219215	23.246757907202316
34	23.009386158134546	25.927982107932902	27.63180253710187	23.43082919683068
35	22.8343390056984	26.06677439340383	27.905851334991805	23.193035265905966
36	22.76684268131919	25.864021556605614	27.868804877044813	23.500330885030383
37	22.897191329651204	24.873952329335623	28.36381310431375	23.865043236699425
38	22.543297388066396	25.293390004211265	28.803251644662197	23.360060963060143
39	22.06248502573726	25.32109568857333	29.16706685988523	23.449352425804175
40	21.91097451182584	24.849835190757595	29.593470535400478	23.64571976201609
41	21.489795336790298	25.002401159311376	29.665399769277617	23.842403734620707
42	21.39322123701395	24.93580197137818	29.577216533908064	24.093760257699806
43	21.105662619701747	24.87701314779848	29.86815260607583	24.14917162642394
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1813.0
1	1434.0
2	1055.0
3	1915.5
4	2776.0
5	2776.0
6	3021.5
7	3267.0
8	2872.5
9	2478.0
10	3437.0
11	4396.0
12	4396.0
13	6719.0
14	9042.0
15	11759.5
16	14477.0
17	15299.5
18	16122.0
19	16122.0
20	20097.5
21	24073.0
22	25466.0
23	26859.0
24	31202.0
25	35545.0
26	35545.0
27	39798.0
28	44051.0
29	51537.5
30	59024.0
31	64357.5
32	69691.0
33	69691.0
34	75472.5
35	81254.0
36	88131.5
37	95009.0
38	99775.0
39	104541.0
40	104541.0
41	110960.5
42	117380.0
43	116557.0
44	115734.0
45	118530.5
46	121327.0
47	121327.0
48	122065.5
49	122804.0
50	126107.0
51	129410.0
52	130719.0
53	132028.0
54	132028.0
55	124095.0
56	116162.0
57	109139.5
58	102117.0
59	94568.5
60	87020.0
61	87020.0
62	80028.0
63	73036.0
64	65006.5
65	56977.0
66	50005.5
67	43034.0
68	43034.0
69	36570.5
70	30107.0
71	25614.5
72	21122.0
73	17261.0
74	13400.0
75	13400.0
76	10591.0
77	7782.0
78	6301.5
79	4821.0
80	3688.5
81	2556.0
82	2556.0
83	1956.5
84	1357.0
85	1017.0
86	677.0
87	491.0
88	305.0
89	305.0
90	233.0
91	161.0
92	112.5
93	64.0
94	48.5
95	33.0
96	33.0
97	26.0
98	19.0
99	15.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1894918.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.466574336759976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.0725540058586	51.89257870151474
2	10.27028852103805	12.830994827187919
3	2.856551869327033	5.353170290763833
4	1.2495489947405503	3.1222018066953714
5	0.6645368043175409	2.075566884320729
6	0.40645124654853815	1.5233770204075807
7	0.2728805161643466	1.1932137733623513
8	0.18370524692759285	0.9180349970604261
9	0.13155479738529	0.7395999777203053
>10	0.7623677690133706	8.816987927388004
>50	0.07415533394527761	3.2302643225173275
>100	0.05149685273878983	5.762752564907276
>500	0.003143425082885363	1.2604124782533084
>1k	5.947020427080412E-4	0.703814327856647
>5k	1.6991486934515463E-4	0.5770301000441354
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5716	0.301648936787766	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5157	0.27214897953367906	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4144	0.21869020189791855	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3034	0.16011246924669034	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.2772732118223585E-5	0.0
3	0.0	0.0	0.0	5.2772732118223585E-5	0.0
4	0.0	0.0	0.0	1.0554546423644717E-4	0.0
5	0.0	0.0	0.0	1.0554546423644717E-4	0.0
6	0.0	0.0	0.0	1.0554546423644717E-4	0.0
7	0.0	0.0	0.0	1.0554546423644717E-4	0.0
8	0.0	0.0	0.0	1.0554546423644717E-4	0.0
9	0.0	0.0	0.0	2.1109092847289434E-4	0.0
10	0.0	0.0	0.0	3.694091248275651E-4	0.0
11	0.0	1.0554546423644717E-4	0.0	3.694091248275651E-4	0.0
12	0.0	1.0554546423644717E-4	0.0	4.749545890640123E-4	5.2772732118223585E-5
13	0.0	1.0554546423644717E-4	0.0	4.749545890640123E-4	5.2772732118223585E-5
14	0.0	1.0554546423644717E-4	0.0	4.749545890640123E-4	5.2772732118223585E-5
15	0.0	1.0554546423644717E-4	0.0	5.277273211822358E-4	5.2772732118223585E-5
16	0.0	1.0554546423644717E-4	0.0	5.805000533004595E-4	5.2772732118223585E-5
17	0.0	1.0554546423644717E-4	0.0	6.33272785418683E-4	5.2772732118223585E-5
18	0.0	1.0554546423644717E-4	0.0	6.860455175369066E-4	5.2772732118223585E-5
19	5.2772732118223585E-5	1.0554546423644717E-4	0.0	8.443637138915774E-4	5.2772732118223585E-5
20	5.2772732118223585E-5	1.0554546423644717E-4	0.0	0.0010026819102462482	5.2772732118223585E-5
21	5.2772732118223585E-5	1.0554546423644717E-4	0.0	0.0011082273744826953	1.0554546423644717E-4
22	5.2772732118223585E-5	1.0554546423644717E-4	0.0	0.0018998183562560491	1.0554546423644717E-4
23	5.2772732118223585E-5	1.5831819635467076E-4	0.0	0.003430227587684533	1.0554546423644717E-4
24	5.2772732118223585E-5	1.5831819635467076E-4	0.0	0.00796868254985176	1.0554546423644717E-4
25	5.2772732118223585E-5	1.5831819635467076E-4	0.0	0.014670819528866157	1.0554546423644717E-4
26	5.2772732118223585E-5	1.5831819635467076E-4	0.0	0.025119820488274425	1.0554546423644717E-4
27	5.2772732118223585E-5	1.5831819635467076E-4	0.0	0.08802491717319694	1.0554546423644717E-4
28	5.2772732118223585E-5	1.5831819635467076E-4	0.0	0.1993753819426487	1.0554546423644717E-4
29	5.2772732118223585E-5	2.1109092847289434E-4	0.0	0.3259771662942671	1.0554546423644717E-4
30	5.2772732118223585E-5	2.1109092847289434E-4	0.0	0.4592810876248999	1.0554546423644717E-4
31	5.2772732118223585E-5	2.1109092847289434E-4	0.0	0.7201367024852791	1.0554546423644717E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1415	0.0	26.540636	1
ATTCGCG	40	0.0019316468	23.125002	30
GTATTAG	320	0.0	17.921875	1
GTATCAA	2180	0.0	17.227064	2
ACGTTAA	175	1.3278623E-10	15.857143	20
CGCGCTT	70	0.0025933261	15.857142	12
TCTAGAC	130	2.5913505E-7	15.653846	3
TTACGCC	85	5.366432E-4	15.235294	34
AACACCG	110	1.4527783E-5	15.136364	7
ATTTACG	100	1.09395834E-4	14.8	32
TTTACGC	75	0.0041057114	14.8	33
TAACGGC	100	1.09395834E-4	14.8	36
TAACGCT	175	2.240995E-9	14.8	32
TTAACGG	100	1.09395834E-4	14.8	35
CGCCGTA	75	0.0041057114	14.8	25
TACGTTA	175	2.240995E-9	14.8	19
CTTAACG	165	1.6467311E-8	14.575757	30
TACCCGT	115	2.2112295E-5	14.478261	6
TTACGTT	185	4.9039954E-9	14.0	18
TAATTCG	205	1.4242687E-9	13.536586	5
>>END_MODULE