FastQCFastQC Report
Fri 10 Feb 2017
ERR1630078.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630078.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577492
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT48410.8382800108053445No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT44630.7728245586086041No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33150.5740339260110963No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24110.4174949609691563No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21850.3783602197086713No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13380.2316915212678271No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12720.22026279151918987No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11430.19792481973776258No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7820.13541313126415605No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7460.1291792786739903No Hit
GACCAACACAGTGAAACCCCACCTCTACTAAAAATACAAAAAT7390.12796714067034695No Hit
CCTGTAATCCCAGCACTTAGGGAGACCAAGGCAGGTGGATCAC7310.12658184009475454No Hit
GGCTGGAGTGCCGTGGCATGATCTCAGCTCACTGCAACCTCTG7260.12571602723500933No Hit
GTGTTGGTCAGGCTGGTCTCAAACTCCTGACCTCGTGATCCAC7200.12467705180331501No Hit
CACTTAGGGAGACCAAGGCAGGTGGATCACGAGGTCAGGAGTT6480.11220934662298351No Hit
GTCTCACTCTGTTGCCCAGGCTGGAGTGCCGTGGCATGATCTC6000.10389754316942917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10000.029.7851
GAGTGAT753.7340942E-722.19999916
CATACAC603.7221536E-521.5833343
GTGTAAT1059.804353E-1021.1428591
GTATAGA450.003823538320.5555551
GCAATAT450.003823538320.55555536
ATACACA3250.019.35384637
GCACGCA701.2178645E-418.59
GCTAGAG1306.9303496E-1018.52
TACACAT1205.156835E-918.537
GTATTGC500.007030747418.4999981
GTAATGG1002.8687646E-718.4999983
TATTGAA500.007030747418.4999982
TCTGTCG500.007030747418.4999988
GAACACT500.007030747418.4999986
CTAATAC500.007030747418.4999983
GTATCAA16550.017.8851972
GGCTAGA1156.388473E-817.6956521
CGCACCT953.600102E-617.52631612
TCTAGCC852.7193179E-517.4117643
Produced by FastQC (version 0.11.5)