##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630078.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	577492
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.733740034493984	34.0	31.0	34.0	31.0	34.0
2	32.97229745173959	34.0	31.0	34.0	31.0	34.0
3	33.08024526746691	34.0	33.0	34.0	31.0	34.0
4	36.369811183531546	37.0	37.0	37.0	35.0	37.0
5	36.33492931503813	37.0	37.0	37.0	35.0	37.0
6	36.4095381407881	37.0	37.0	37.0	35.0	37.0
7	36.317484917539986	37.0	37.0	37.0	35.0	37.0
8	36.31210129317809	37.0	37.0	37.0	35.0	37.0
9	38.10130703109307	39.0	39.0	39.0	37.0	39.0
10	38.08114571284104	39.0	38.0	39.0	37.0	39.0
11	38.11995490846626	39.0	39.0	39.0	37.0	39.0
12	38.03850096624715	39.0	38.0	39.0	35.0	39.0
13	38.03783948522231	39.0	38.0	39.0	35.0	39.0
14	39.42482666426548	41.0	39.0	41.0	36.0	41.0
15	39.40170772928456	41.0	39.0	41.0	36.0	41.0
16	39.294798888988936	41.0	39.0	41.0	36.0	41.0
17	39.21491726292312	40.0	39.0	41.0	36.0	41.0
18	39.113033600465464	40.0	39.0	41.0	36.0	41.0
19	39.131141210614174	40.0	39.0	41.0	36.0	41.0
20	39.37681560956688	41.0	39.0	41.0	36.0	41.0
21	39.32976560714261	41.0	39.0	41.0	36.0	41.0
22	39.28041773738857	41.0	39.0	41.0	36.0	41.0
23	39.17627603499269	41.0	39.0	41.0	35.0	41.0
24	39.1474461983889	41.0	39.0	41.0	35.0	41.0
25	39.07996301247463	41.0	39.0	41.0	35.0	41.0
26	38.9280977052496	41.0	39.0	41.0	35.0	41.0
27	38.70906609961696	40.0	38.0	41.0	35.0	41.0
28	38.69136888476377	40.0	38.0	41.0	35.0	41.0
29	38.51007113518455	40.0	38.0	41.0	35.0	41.0
30	38.40026874831167	40.0	38.0	41.0	35.0	41.0
31	38.17755224314796	40.0	38.0	41.0	34.0	41.0
32	38.03156753686631	40.0	38.0	41.0	34.0	41.0
33	37.86587519827115	40.0	37.0	41.0	33.0	41.0
34	37.8200719663649	40.0	37.0	41.0	33.0	41.0
35	37.60219362346145	40.0	37.0	41.0	33.0	41.0
36	37.462222853303594	40.0	37.0	41.0	33.0	41.0
37	37.3945145560458	40.0	37.0	41.0	32.0	41.0
38	37.20669204075554	40.0	36.0	41.0	31.0	41.0
39	37.14408164961593	40.0	36.0	41.0	31.0	41.0
40	37.02201242614616	40.0	36.0	41.0	31.0	41.0
41	36.89416476765046	40.0	36.0	41.0	31.0	41.0
42	36.74131936026819	40.0	35.0	41.0	30.0	41.0
43	36.359279435905606	40.0	35.0	41.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	6.0
16	7.0
17	23.0
18	42.0
19	82.0
20	147.0
21	297.0
22	467.0
23	752.0
24	1061.0
25	1496.0
26	2126.0
27	2939.0
28	4050.0
29	5247.0
30	6549.0
31	8086.0
32	10296.0
33	13532.0
34	17765.0
35	24133.0
36	37305.0
37	62115.0
38	105476.0
39	273490.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.12460778677453	17.83938132476294	11.745097767588122	25.290913120874404
2	22.483428341864474	21.237869961834967	31.630394879929074	24.64830681637148
3	21.694499664064608	19.999411247255374	29.86552194662437	28.440567142055645
4	16.091651486081194	13.420618813767119	33.132753354159014	37.354976345992675
5	15.743248391319705	34.431472643776885	33.35907683569643	16.466202129206987
6	36.9303817195736	33.021756145539676	12.861650031515588	17.18621210337113
7	29.059450174201544	29.246119426762622	19.681138440013022	22.01329195902281
8	26.891281610827512	30.736356520956132	20.56218960608978	21.810172262126574
9	25.528492169588496	12.38077756921308	20.112832731881998	41.97789752931642
10	17.724228214416822	25.683818996626794	32.536208293794544	24.05574449516184
11	34.36740249215573	20.035255899648828	19.542435219881835	26.054906388313604
12	22.292949512720522	25.64780118166139	28.13406938970583	23.925179915912256
13	32.833874754974964	18.36960512007093	22.45762711864407	26.338893006310045
14	23.563789628254593	21.138820970680115	25.477582373435474	29.819807027629817
15	27.782203043505366	26.02650772651396	23.36586480851683	22.825424421463847
16	25.082598546819696	25.93213412480173	22.56273679981714	26.422530528561435
17	21.72705422759103	23.943188823394955	26.130751594827288	28.199005354186724
18	22.100392732713182	23.31841826380279	27.431721997880494	27.14946700560354
19	23.781281818622595	22.74092108635271	28.406800440525583	25.070996654499112
20	26.94167191926468	23.755480595402187	26.688335076503222	22.614512408829906
21	26.46686014698039	23.96968269690316	24.670817950724857	24.89263920539159
22	24.413498368808572	24.201720543314885	27.321763764692847	24.063017323183697
23	24.52674669086325	24.486053486455223	25.550830141369925	25.436369681311604
24	26.005035567592277	23.20205301545303	27.205744841486982	23.587166575467712
25	24.76501838986514	24.607959937107353	26.074993246659695	24.552028426367812
26	24.381463292998	23.688639842629854	25.74962077396743	26.180276090404714
27	23.577642634010516	23.28378574941298	27.823588898201184	25.31498271837532
28	23.911673235300228	23.798078588101653	26.034473204823616	26.2557749717745
29	23.055211154440236	24.084835807249277	28.17216515553462	24.687787882775865
30	24.047952179424133	26.209540565064103	25.81005451157765	23.932452743934114
31	25.627021673027507	25.958108510594087	25.649532807380883	22.765337008997527
32	22.375894384684116	25.534552859606713	26.824960345771025	25.264592409938146
33	23.342487861303706	24.228907067110885	28.296842207337935	24.13176286424747
34	23.018154364043138	22.17017724920865	29.14984103675895	25.661827349989263
35	22.321694499664066	23.665436057988682	28.73927257866776	25.273596863679497
36	23.433917699292802	25.334896414149462	27.429124559301254	23.80206132725648
37	24.458001149799475	24.560859717537213	26.57664521759609	24.40449391506722
38	23.4896760474604	25.167101881930833	27.689907392656522	23.65331467795225
39	22.12047959105927	23.90214929384303	29.103260304904655	24.87411081019304
40	20.943493589521587	23.04689935098668	30.914887132635606	25.09471992685613
41	21.54731147790792	22.705769084247056	30.286999646748352	25.459919791096674
42	20.93016007148151	23.27097171908875	30.186738517589855	25.612129691839886
43	20.71180206825376	22.281867108115783	30.80163881058093	26.20469201304953
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3859.0
1	2593.0
2	1327.0
3	1781.5
4	2236.0
5	2236.0
6	2312.5
7	2389.0
8	1946.0
9	1503.0
10	2025.5
11	2548.0
12	2548.0
13	4259.5
14	5971.0
15	7504.5
16	9038.0
17	8164.0
18	7290.0
19	7290.0
20	8325.5
21	9361.0
22	7496.5
23	5632.0
24	5413.5
25	5195.0
26	5195.0
27	5531.5
28	5868.0
29	7202.5
30	8537.0
31	9813.5
32	11090.0
33	11090.0
34	12370.5
35	13651.0
36	15744.5
37	17838.0
38	18129.0
39	18420.0
40	18420.0
41	19660.0
42	20900.0
43	20968.5
44	21037.0
45	21660.0
46	22283.0
47	22283.0
48	23936.0
49	25589.0
50	29988.5
51	34388.0
52	40022.0
53	45656.0
54	45656.0
55	49850.5
56	54045.0
57	53886.5
58	53728.0
59	53317.5
60	52907.0
61	52907.0
62	47422.5
63	41938.0
64	35275.5
65	28613.0
66	23789.0
67	18965.0
68	18965.0
69	15185.0
70	11405.0
71	8981.0
72	6557.0
73	5118.5
74	3680.0
75	3680.0
76	2767.0
77	1854.0
78	1484.0
79	1114.0
80	804.5
81	495.0
82	495.0
83	391.0
84	287.0
85	223.5
86	160.0
87	113.0
88	66.0
89	66.0
90	51.0
91	36.0
92	29.5
93	23.0
94	14.5
95	6.0
96	6.0
97	4.0
98	2.0
99	3.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	577492.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.735000165863546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.39400898105686	36.637440227324014
2	11.050614592302198	10.329009496082747
3	3.694865545198863	5.180386256031372
4	1.8170440722483003	3.396782200716228
5	1.0423691486705748	2.4357561168005177
6	0.6773172667292134	1.899265354375922
7	0.4781120532348797	1.5641196821063426
8	0.36240055757897166	1.3549432094849827
9	0.28823567498435004	1.2123624886381252
>10	1.906889902163337	17.27016984480461
>50	0.18835755100664064	6.137919970103916
>100	0.09305759944435495	7.747241984980121
>500	0.0037372529897331305	1.1723214041659884
>1k	0.002989802391786504	3.6622817643850483
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4841	0.8382800108053445	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4463	0.7728245586086041	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3315	0.5740339260110963	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2411	0.4174949609691563	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2185	0.3783602197086713	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1338	0.2316915212678271	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1272	0.22026279151918987	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1143	0.19792481973776258	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	782	0.13541313126415605	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	746	0.1291792786739903	No Hit
GACCAACACAGTGAAACCCCACCTCTACTAAAAATACAAAAAT	739	0.12796714067034695	No Hit
CCTGTAATCCCAGCACTTAGGGAGACCAAGGCAGGTGGATCAC	731	0.12658184009475454	No Hit
GGCTGGAGTGCCGTGGCATGATCTCAGCTCACTGCAACCTCTG	726	0.12571602723500933	No Hit
GTGTTGGTCAGGCTGGTCTCAAACTCCTGACCTCGTGATCCAC	720	0.12467705180331501	No Hit
CACTTAGGGAGACCAAGGCAGGTGGATCACGAGGTCAGGAGTT	648	0.11220934662298351	No Hit
GTCTCACTCTGTTGCCCAGGCTGGAGTGCCGTGGCATGATCTC	600	0.10389754316942917	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	3.463251438980973E-4	0.0
11	0.0	0.0	0.0	5.194877158471459E-4	0.0
12	0.0	0.0	0.0	5.194877158471459E-4	0.0
13	0.0	0.0	0.0	5.194877158471459E-4	0.0
14	0.0	0.0	0.0	5.194877158471459E-4	0.0
15	0.0	0.0	0.0	5.194877158471459E-4	0.0
16	0.0	0.0	0.0	6.926502877961946E-4	0.0
17	0.0	0.0	0.0	6.926502877961946E-4	0.0
18	0.0	0.0	0.0	0.0010389754316942918	0.0
19	0.0	0.0	0.0	0.0013853005755923892	0.0
20	0.0	0.0	0.0	0.0019047882914395352	0.0
21	0.0	0.0	0.0	0.0022511134353376326	0.0
22	0.0	0.0	0.0	0.004155901726777167	0.0
23	0.0	0.0	0.0	0.006580177734063848	0.0
24	0.0	0.0	0.0	0.014545656043720085	0.0
25	0.0	0.0	0.0	0.029783962375236367	0.0
26	0.0	0.0	0.0	0.050390308437173154	0.0
27	0.0	0.0	0.0	0.1939420805829345	0.0
28	0.0	0.0	0.0	0.434291730448214	0.0
29	0.0	0.0	0.0	0.6968061895229718	0.0
30	0.0	0.0	0.0	0.9749052800731439	0.0
31	0.0	0.0	0.0	1.3407977946014837	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1000	0.0	29.785	1
GAGTGAT	75	3.7340942E-7	22.199999	16
CATACAC	60	3.7221536E-5	21.583334	3
GTGTAAT	105	9.804353E-10	21.142859	1
GTATAGA	45	0.0038235383	20.555555	1
GCAATAT	45	0.0038235383	20.555555	36
ATACACA	325	0.0	19.353846	37
GCACGCA	70	1.2178645E-4	18.5	9
GCTAGAG	130	6.9303496E-10	18.5	2
TACACAT	120	5.156835E-9	18.5	37
GTATTGC	50	0.0070307474	18.499998	1
GTAATGG	100	2.8687646E-7	18.499998	3
TATTGAA	50	0.0070307474	18.499998	2
TCTGTCG	50	0.0070307474	18.499998	8
GAACACT	50	0.0070307474	18.499998	6
CTAATAC	50	0.0070307474	18.499998	3
GTATCAA	1655	0.0	17.885197	2
GGCTAGA	115	6.388473E-8	17.695652	1
CGCACCT	95	3.600102E-6	17.526316	12
TCTAGCC	85	2.7193179E-5	17.411764	3
>>END_MODULE