FastQCFastQC Report
Fri 10 Feb 2017
ERR1630077.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630077.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences900627
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29970.3327681715071833No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26430.2934622213191477No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20330.22573162918722178No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14600.1621092860862488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTT308.309989E-637.024
GACGTTA352.387738E-531.71428725
TTACTCG454.00699E-628.77777929
GGTATCA6200.024.766131
GTATTAG1705.456968E-1217.4117641
TAGCGGT752.0666409E-417.26666618
ACGTTAC650.001579574917.07692326
CCCCGAA1201.04042556E-716.95833212
CTAACAC1352.2171662E-816.4444433
CGGTCGA700.00259190915.85714320
TGTAGCG855.3625496E-415.23529416
GTGTGTA2350.014.9574469
GTATCAA11000.014.81
GGTGTGT2500.014.7999998
TCGGCGT750.004103489714.79999911
CGTTACT750.004103489714.79999927
TCGGACT750.004103489714.79999933
GTCGTGT1001.0929465E-414.79999912
GTGTAAG1252.95684E-614.7999991
GTAAACG1001.0929465E-414.79999912
Produced by FastQC (version 0.11.5)