FastQCFastQC Report
Fri 10 Feb 2017
ERR1630076.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630076.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53602
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11282.1043990895862095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10591.9756725495317338No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6811.2704749822767807No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4190.7816872504757285No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.223872243573001No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.220141039513451No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1100.20521622327525094No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1100.20521622327525094No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080.2014850192157009No Hit
GAGTACGGGGGAGGCAGTACAGGCACTAAAAAAAAAAAAAAAA1040.19402261109660088No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT970.1809633968881758TruSeq Adapter, Index 3 (95% over 21bp)
AGGCAGTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAA970.1809633968881758No Hit
GTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA910.16976978470952575No Hit
CAGTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA790.14738256035222566No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT790.14738256035222566No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG700.13059214208425057No Hit
TGCCTGTACTGCCTCCCCCGTACTCTGCGTTGATACCCTGTCT650.12126413193537554No Hit
GCAGTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.11939852990560053No Hit
GAGTACGGGGGAGGCAGTACAGGCAAAAAAAAAAAAAAAAAAA590.1100705197567255No Hit
GTACGGGGGAGGCAGTACAGGCACTAAAAAAAAAAAAAAAAAA570.10633931569717547No Hit
GGTATCAACGCAGAGTACGGGGGAGGCAGTACAGGCACTGTCT540.10074250960785044No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGCG200.001826326637.07
GGTATCA3300.020.1818181
GGGAAGC700.00254952215.85714219
CTTATAC800.006195929413.87537
CGGGAAG800.006195929413.87518
GTATCAA4900.013.5918362
CTAAAAA1602.5615354E-612.7187510
TCTCTTA2357.2759576E-1012.59574537
ACGGGAA1050.002587314712.33333417
ACTAAAA1653.6157635E-612.3333339
TAAAAAA1705.0479302E-611.97058911
TATCAAC7200.011.0486121
AACGCAG7200.011.0486125
CGCAGAG7100.010.9436627
TCAACGC7300.010.897263
GGCACTA1706.696388E-510.8823536
ATCAACG7350.010.8231292
CTCTTAT1553.4771187E-410.74193637
CACTAAA1901.7382785E-510.7105268
CAACGCA7450.010.6778524
Produced by FastQC (version 0.11.5)