##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630076.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	53602
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6426252751763	33.0	31.0	34.0	31.0	34.0
2	32.8383269280997	34.0	31.0	34.0	31.0	34.0
3	32.91981642476027	34.0	31.0	34.0	31.0	34.0
4	36.216988172083134	37.0	35.0	37.0	35.0	37.0
5	36.24428192977874	37.0	37.0	37.0	35.0	37.0
6	36.356162083504344	37.0	37.0	37.0	35.0	37.0
7	36.25204283422261	37.0	37.0	37.0	35.0	37.0
8	36.305025931868215	37.0	37.0	37.0	35.0	37.0
9	38.038095593448006	39.0	38.0	39.0	35.0	39.0
10	38.04180814148726	39.0	38.0	39.0	35.0	39.0
11	38.08999664191634	39.0	39.0	39.0	37.0	39.0
12	37.99824633409201	39.0	38.0	39.0	35.0	39.0
13	38.02535353158464	39.0	38.0	39.0	35.0	39.0
14	39.29329129510093	41.0	39.0	41.0	36.0	41.0
15	39.28679900003731	41.0	39.0	41.0	36.0	41.0
16	39.14518114995709	40.0	39.0	41.0	36.0	41.0
17	39.176672512219696	40.0	39.0	41.0	36.0	41.0
18	39.135256147158685	40.0	39.0	41.0	36.0	41.0
19	39.168090742882725	41.0	39.0	41.0	36.0	41.0
20	39.367150479459724	41.0	39.0	41.0	36.0	41.0
21	39.338159023917015	41.0	39.0	41.0	36.0	41.0
22	39.29554867355696	41.0	39.0	41.0	36.0	41.0
23	39.18266109473527	41.0	39.0	41.0	35.0	41.0
24	39.0771239879109	41.0	39.0	41.0	35.0	41.0
25	39.03518525428156	41.0	39.0	41.0	35.0	41.0
26	38.75842319316443	40.0	38.0	41.0	35.0	41.0
27	38.56639677623969	40.0	38.0	41.0	35.0	41.0
28	38.39345546807955	40.0	38.0	41.0	35.0	41.0
29	38.184041640237304	40.0	37.0	41.0	34.0	41.0
30	37.895731502555876	40.0	37.0	41.0	34.0	41.0
31	37.60070519756726	40.0	36.0	41.0	33.0	41.0
32	37.349445916197155	40.0	35.0	41.0	33.0	41.0
33	37.1127756426999	40.0	35.0	41.0	33.0	41.0
34	36.88698182903623	39.0	35.0	41.0	33.0	41.0
35	36.567553449498156	39.0	35.0	41.0	31.0	41.0
36	36.355602402895414	39.0	35.0	41.0	31.0	41.0
37	36.109398903026005	39.0	35.0	41.0	30.0	41.0
38	35.81325323681952	39.0	35.0	41.0	28.0	41.0
39	35.509104137905304	39.0	35.0	41.0	26.0	41.0
40	35.180198500055965	38.0	35.0	41.0	24.0	41.0
41	34.784187157195625	38.0	35.0	41.0	21.0	41.0
42	34.401887989254135	38.0	35.0	41.0	18.0	41.0
43	33.85563971493601	37.0	34.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	4.0
18	2.0
19	9.0
20	15.0
21	27.0
22	46.0
23	80.0
24	125.0
25	208.0
26	286.0
27	434.0
28	587.0
29	651.0
30	831.0
31	968.0
32	1214.0
33	1661.0
34	2037.0
35	2649.0
36	3730.0
37	8171.0
38	10380.0
39	19484.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.854520353718144	17.670982426028882	13.411812992052536	20.06268422820044
2	22.090593634565874	23.056975485989327	31.883138688854896	22.969292190589904
3	23.14279317935898	24.39834334539756	30.62012611469721	21.838737360546247
4	14.337151598820942	16.939666430357075	32.19469422782732	36.52848774299466
5	14.424834894220364	38.2355136002388	31.61449199656729	15.725159508973546
6	33.08085519197045	33.62561098466475	13.74575575538226	19.547778067982538
7	26.907578075444942	30.814148725793817	20.641020857430693	21.637252341330548
8	29.73209954852431	31.27308682511847	17.691504048356403	21.30330957800082
9	25.54195738964964	13.45472183873736	19.62986455729264	41.373456214320356
10	20.456326256482967	26.844147606432596	27.470989888436996	25.228536248647437
11	36.09193686802731	21.80888772806985	19.488078803029737	22.6110966008731
12	21.8126189321294	28.64072236110593	26.489683220775344	23.056975485989327
13	33.905451289131	19.51046602738704	23.573747248237005	23.01033543524495
14	21.698817208313123	22.723032722659603	27.81052945785605	27.767620611171225
15	26.534457669489946	27.325472930114547	23.417036677735904	22.723032722659603
16	21.8499309727249	26.232230140666392	27.109063094660645	24.80877579194806
17	22.3424499085855	27.984030446625123	26.247154956904595	23.426364687884778
18	22.661467855677028	24.131562255139734	28.0381329054886	25.168836983694636
19	24.722958098578413	25.07555688220589	29.82911085407261	20.372374165143093
20	24.056938173948733	24.366628110891384	29.65001305921421	21.926420655945673
21	23.439423902093207	25.325547554195737	29.860826088578783	21.37420245513227
22	23.01220103727473	26.133353233088318	29.571657773963654	21.282787955673296
23	22.66706466176635	25.97104585649789	29.508227304951305	21.85366217678445
24	22.0961904406552	25.614715868810865	30.153725607253463	22.135368083280476
25	21.512257005335623	25.868437744860266	30.039923883437186	22.579381366366928
26	21.823812544308048	26.332972650274243	30.55483004365509	21.28838476176262
27	21.835006156486696	26.48035521062647	30.416775493451738	21.267863139435093
28	20.898473937539645	27.648222081265622	30.664900563411813	20.78840341778292
29	21.52345061751427	27.377709786948245	30.202231260027613	20.89660833550987
30	20.23805081899929	28.226558710495876	30.636916532965188	20.898473937539645
31	20.618633633073394	28.058654527816124	30.19290324987874	21.129808589231743
32	21.320099996268794	26.844147606432596	30.40371627924331	21.432036118055297
33	20.62236483713294	27.428081041752172	30.00820864893101	21.941345472183873
34	21.06451251818962	27.036304615499425	29.933584567740006	21.96559829857095
35	20.19700757434424	27.41688742957352	30.713406216185962	21.672698779896272
36	20.47311667475094	26.95421812618932	30.933547255699413	21.639117943360322
37	20.226857206820643	26.575500914144996	31.217118764225216	21.98052311480915
38	19.555240476101638	26.43558076191187	31.48949666057237	22.519682101414126
39	19.37427707921346	26.586694526323644	31.935375545688593	22.1036528487743
40	19.66717659788814	26.321779038095595	32.09395171821947	21.9170926457968
41	19.019812693556208	26.534457669489946	32.24693108466102	22.198798552292825
42	18.614977053095032	26.68184022984217	31.52680870116787	23.17637401589493
43	18.64669228760121	26.277004589380994	31.681653669639193	23.394649453378605
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	106.0
1	88.0
2	70.0
3	155.5
4	241.0
5	241.0
6	286.0
7	331.0
8	327.5
9	324.0
10	419.0
11	514.0
12	514.0
13	944.5
14	1375.0
15	1928.5
16	2482.0
17	2149.0
18	1816.0
19	1816.0
20	2002.5
21	2189.0
22	1471.0
23	753.0
24	704.0
25	655.0
26	655.0
27	611.0
28	567.0
29	692.0
30	817.0
31	868.0
32	919.0
33	919.0
34	1000.0
35	1081.0
36	1232.0
37	1383.0
38	1489.0
39	1595.0
40	1595.0
41	1695.0
42	1795.0
43	1926.0
44	2057.0
45	2348.5
46	2640.0
47	2640.0
48	2861.0
49	3082.0
50	3239.0
51	3396.0
52	3347.5
53	3299.0
54	3299.0
55	3312.0
56	3325.0
57	3389.0
58	3453.0
59	3279.0
60	3105.0
61	3105.0
62	2939.0
63	2773.0
64	2537.0
65	2301.0
66	2054.5
67	1808.0
68	1808.0
69	1575.5
70	1343.0
71	1092.5
72	842.0
73	677.0
74	512.0
75	512.0
76	424.5
77	337.0
78	251.5
79	166.0
80	123.0
81	80.0
82	80.0
83	57.0
84	34.0
85	27.5
86	21.0
87	15.5
88	10.0
89	10.0
90	7.5
91	5.0
92	2.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	53602.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.92261482780494
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.71199273167777	64.83899854483042
2	8.545326065011103	12.633856945636357
3	2.046739349888956	4.539009738442595
4	0.7217847769028871	2.1342487220626096
5	0.27760952957803353	1.0260811163762547
6	0.1539471027660004	0.6828103428976531
7	0.09842519685039369	0.5093093541285774
8	0.06056935190793458	0.3581955897168016
9	0.03028467595396729	0.2014850192157009
>10	0.29779931354734507	4.0838028431774935
>50	0.03028467595396729	1.6100145516958324
>100	0.017666060973147588	2.031640610424984
>500	0.002523722996163941	1.2704749822767807
>1k	0.005047445992327882	4.080071639117944
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1128	2.1043990895862095	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1059	1.9756725495317338	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	681	1.2704749822767807	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	419	0.7816872504757285	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	120	0.223872243573001	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	118	0.220141039513451	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	110	0.20521622327525094	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	110	0.20521622327525094	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	108	0.2014850192157009	No Hit
GAGTACGGGGGAGGCAGTACAGGCACTAAAAAAAAAAAAAAAA	104	0.19402261109660088	No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	97	0.1809633968881758	TruSeq Adapter, Index 3 (95% over 21bp)
AGGCAGTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAA	97	0.1809633968881758	No Hit
GTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	91	0.16976978470952575	No Hit
CAGTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	79	0.14738256035222566	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT	79	0.14738256035222566	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	70	0.13059214208425057	No Hit
TGCCTGTACTGCCTCCCCCGTACTCTGCGTTGATACCCTGTCT	65	0.12126413193537554	No Hit
GCAGTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	64	0.11939852990560053	No Hit
GAGTACGGGGGAGGCAGTACAGGCAAAAAAAAAAAAAAAAAAA	59	0.1100705197567255	No Hit
GTACGGGGGAGGCAGTACAGGCACTAAAAAAAAAAAAAAAAAA	57	0.10633931569717547	No Hit
GGTATCAACGCAGAGTACGGGGGAGGCAGTACAGGCACTGTCT	54	0.10074250960785044	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.003731204059550017	0.0
11	0.0	0.0	0.0	0.003731204059550017	0.0
12	0.0	0.0	0.0	0.003731204059550017	0.0
13	0.0	0.0	0.0	0.003731204059550017	0.0
14	0.0	0.0	0.0	0.003731204059550017	0.0
15	0.0	0.0	0.0	0.003731204059550017	0.0
16	0.0	0.0	0.0	0.003731204059550017	0.0
17	0.0	0.0	0.0	0.003731204059550017	0.0
18	0.0	0.0	0.0	0.003731204059550017	0.0
19	0.0	0.0	0.0	0.003731204059550017	0.0
20	0.0	0.0	0.0	0.003731204059550017	0.0
21	0.0	0.0	0.0	0.003731204059550017	0.0
22	0.0	0.0	0.0	0.003731204059550017	0.0
23	0.0	0.0	0.0	0.007462408119100034	0.0
24	0.0	0.0	0.0	0.016790418267975074	0.0
25	0.0	0.0	0.0	0.04104324465505019	0.0
26	0.0	0.0	0.0	0.0671616730719003	0.0
27	0.0	0.0	0.0	0.22946904966232604	0.0
28	0.0	0.0	0.0	0.4290884668482519	0.0
29	0.0	0.0	0.0	0.662288720570128	0.0
30	0.0	0.0	0.0	0.9607850453341293	0.0
31	0.0	0.0	0.0	1.4439759710458564	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGGCG	20	0.0018263266	37.0	7
GGTATCA	330	0.0	20.181818	1
GGGAAGC	70	0.002549522	15.857142	19
CTTATAC	80	0.0061959294	13.875	37
CGGGAAG	80	0.0061959294	13.875	18
GTATCAA	490	0.0	13.591836	2
CTAAAAA	160	2.5615354E-6	12.71875	10
TCTCTTA	235	7.2759576E-10	12.595745	37
ACGGGAA	105	0.0025873147	12.333334	17
ACTAAAA	165	3.6157635E-6	12.333333	9
TAAAAAA	170	5.0479302E-6	11.970589	11
TATCAAC	720	0.0	11.048612	1
AACGCAG	720	0.0	11.048612	5
CGCAGAG	710	0.0	10.943662	7
TCAACGC	730	0.0	10.89726	3
GGCACTA	170	6.696388E-5	10.882353	6
ATCAACG	735	0.0	10.823129	2
CTCTTAT	155	3.4771187E-4	10.741936	37
CACTAAA	190	1.7382785E-5	10.710526	8
CAACGCA	745	0.0	10.677852	4
>>END_MODULE