FastQCFastQC Report
Fri 10 Feb 2017
ERR1630075.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630075.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679066
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35520.5230713951221236No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30270.4457593223633638No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23380.3442964306856771No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14930.21986080881681602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8500.025.4647061
GCATAGA603.7233953E-521.5833341
TAACGTA450.00382422120.55555532
AACGTAA555.140114E-420.18181833
ATAGGTG759.256615E-619.7333345
TACACTG1500.019.7333345
ACCGCGC609.231508E-418.518
TGTATAC609.231508E-418.53
TTAACGT609.231508E-418.531
AGCGTTA500.00703199218.49999819
CTAGACT500.00703199218.4999984
ACTTCGT500.00703199218.4999988
CGTCAGA650.001579033717.0769231
GTGCTAG1757.2759576E-1216.9142861
GTATCAA13150.016.4600752
GTATTAG1251.6546619E-716.281
TGCTCGG803.380017E-416.187515
TCTATAC803.380017E-416.18753
TCGCCAT1504.658432E-916.03333313
TAGACTT700.002591028615.8571435
Produced by FastQC (version 0.11.5)