##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630075.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	679066
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83901417535262	34.0	31.0	34.0	31.0	34.0
2	33.05796638323815	34.0	33.0	34.0	31.0	34.0
3	33.17190376193183	34.0	33.0	34.0	31.0	34.0
4	36.45273508024257	37.0	37.0	37.0	35.0	37.0
5	36.41466219778343	37.0	37.0	37.0	35.0	37.0
6	36.47267423195978	37.0	37.0	37.0	35.0	37.0
7	36.40465286143026	37.0	37.0	37.0	35.0	37.0
8	36.410622826058145	37.0	37.0	37.0	35.0	37.0
9	38.18155525383394	39.0	39.0	39.0	37.0	39.0
10	38.164995744154474	39.0	39.0	39.0	37.0	39.0
11	38.20360318437383	39.0	39.0	39.0	37.0	39.0
12	38.15398797760454	39.0	39.0	39.0	37.0	39.0
13	38.15889913498835	39.0	39.0	39.0	37.0	39.0
14	39.61221147870752	41.0	40.0	41.0	37.0	41.0
15	39.60541537935929	41.0	40.0	41.0	37.0	41.0
16	39.506874147726435	41.0	39.0	41.0	37.0	41.0
17	39.460981701336834	41.0	39.0	41.0	37.0	41.0
18	39.36715871505862	41.0	39.0	41.0	36.0	41.0
19	39.410080905243376	41.0	39.0	41.0	36.0	41.0
20	39.62064512138732	41.0	40.0	41.0	37.0	41.0
21	39.612563432714936	41.0	40.0	41.0	37.0	41.0
22	39.62721149343363	41.0	40.0	41.0	37.0	41.0
23	39.56052578099919	41.0	40.0	41.0	37.0	41.0
24	39.5285539255389	41.0	40.0	41.0	37.0	41.0
25	39.495253775038066	41.0	40.0	41.0	37.0	41.0
26	39.35850565335328	41.0	39.0	41.0	36.0	41.0
27	39.26394047117659	41.0	39.0	41.0	36.0	41.0
28	39.22137023499925	41.0	39.0	41.0	36.0	41.0
29	39.110325064132205	41.0	39.0	41.0	35.0	41.0
30	39.02545849740673	41.0	39.0	41.0	35.0	41.0
31	38.88573717429528	40.0	39.0	41.0	35.0	41.0
32	38.782215572565846	40.0	39.0	41.0	35.0	41.0
33	38.7066838863969	40.0	38.0	41.0	35.0	41.0
34	38.63746970103053	40.0	38.0	41.0	35.0	41.0
35	38.48639012997264	40.0	38.0	41.0	35.0	41.0
36	38.46934465869297	40.0	38.0	41.0	35.0	41.0
37	38.411811223062266	40.0	38.0	41.0	35.0	41.0
38	38.28841820971746	40.0	38.0	41.0	35.0	41.0
39	38.228565411904	40.0	38.0	41.0	34.0	41.0
40	38.16416813682322	40.0	38.0	41.0	34.0	41.0
41	38.046774540324506	40.0	38.0	41.0	34.0	41.0
42	37.94129583869609	40.0	38.0	41.0	33.0	41.0
43	37.638260493089035	40.0	38.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	4.0
15	4.0
16	6.0
17	29.0
18	46.0
19	88.0
20	105.0
21	201.0
22	318.0
23	493.0
24	760.0
25	1142.0
26	1669.0
27	2145.0
28	3003.0
29	4014.0
30	5368.0
31	6908.0
32	8737.0
33	11454.0
34	15414.0
35	21133.0
36	31857.0
37	58376.0
38	119082.0
39	386709.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.26030164961875	19.472923103203517	12.578747868395709	24.68802737878203
2	18.768573305098474	22.178845649760113	35.17507871105312	23.87750233408829
3	19.365422506796097	23.348540495327406	31.595603372868027	25.690433625008467
4	14.569717818297484	16.35466950193354	35.38360041586532	33.692012263903656
5	13.843131595456112	36.85856750301149	35.3065828652885	13.991718036243899
6	33.249345424450645	35.70595494399661	15.571240497978106	15.473459133574647
7	27.37377515587586	31.28502973201427	22.088721862087045	19.252473250022824
8	25.439353464906212	34.48736941622758	20.99147947327653	19.081797645589678
9	25.046902657473645	14.948473344269924	20.93979083034639	39.06483316791004
10	16.09107214909891	27.737362789478485	34.78410051453025	21.38746454689235
11	33.06644714946706	22.912500404968	22.307993626539982	21.713058819024955
12	19.926634524479212	26.633788173756308	30.851198558019398	22.588378743745086
13	29.261073297735418	21.251100776654994	25.65229300244748	23.83553292316211
14	21.656216037910895	22.859780934401076	26.59373315701272	28.89026987067531
15	24.22179876477397	28.591035333826163	24.1868978862143	23.00026801518556
16	22.90425378387373	28.318455054442428	25.368962663422995	23.408328498260847
17	21.938810071480532	28.0143609015913	26.996934024086023	23.04989500284214
18	21.596575296068423	26.8951766102264	28.36484229809768	23.143405795607496
19	23.100405557044528	26.469739318416767	28.959777105612712	21.470078018925996
20	23.526579154309008	26.234121572866258	28.727104581881584	21.512194690943147
21	22.56098818082484	26.74393946980117	27.685674146548344	23.009398202825647
22	22.282811979984274	26.773244426904014	27.967973657936046	22.97596993517567
23	22.537573667360757	26.91122806914203	27.796267225866117	22.7549310376311
24	22.979798723540863	26.811237788374033	28.10315933944565	22.105804148639454
25	23.03561067701814	26.533061587533464	27.80672276332492	22.624604972123475
26	22.694553990333784	26.93714602115258	27.820123522603108	22.54817646591053
27	22.69661564560735	26.736576415252717	27.364055923871906	23.20275201526803
28	22.261900905066664	26.909313674959428	27.95795990375015	22.870825516223757
29	22.249530973425262	27.022999237187552	28.298280284979665	22.429189504407525
30	22.29768535017215	27.802599452777788	27.90141164481803	21.998303552232038
31	22.80367445874186	27.092948255397854	28.031295927052746	22.072081358807537
32	21.70613754774941	27.09353729976173	28.215225029673107	22.985100122815748
33	22.122297390827992	26.72774074979457	28.48721626469298	22.662745594684463
34	22.19769506940415	26.1386963859183	28.688669437138657	22.974939107538884
35	22.120972041009267	26.448975504590127	28.76377259353288	22.66627986086772
36	22.2046163406797	26.29302600925389	28.98746219071489	22.51489545935152
37	22.602368547387147	25.587056339148184	28.557018021812315	23.253557091652358
38	21.975036299858925	26.082000865895218	29.39714254579084	22.545820288455026
39	21.68345933974017	25.811334980694074	29.521725428750667	22.98348025081509
40	21.018722775105807	25.50120312311322	30.239917769406805	23.240156332374173
41	20.69636824697452	25.36027425905582	30.610426674284973	23.332930819684687
42	20.941999746710923	24.917754680693776	30.28439061887946	23.855854953715838
43	20.482692403978405	25.14262236660354	30.556381853899328	23.818303375518727
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	377.0
1	359.5
2	342.0
3	656.5
4	971.0
5	971.0
6	1180.5
7	1390.0
8	1258.5
9	1127.0
10	1576.0
11	2025.0
12	2025.0
13	3333.5
14	4642.0
15	6264.0
16	7886.0
17	7874.0
18	7862.0
19	7862.0
20	9105.5
21	10349.0
22	10088.0
23	9827.0
24	10870.0
25	11913.0
26	11913.0
27	13616.0
28	15319.0
29	18962.5
30	22606.0
31	24702.0
32	26798.0
33	26798.0
34	28397.5
35	29997.0
36	31751.0
37	33505.0
38	35741.0
39	37977.0
40	37977.0
41	39768.5
42	41560.0
43	39978.0
44	38396.0
45	39652.0
46	40908.0
47	40908.0
48	41450.5
49	41993.0
50	45166.5
51	48340.0
52	50935.0
53	53530.0
54	53530.0
55	49675.0
56	45820.0
57	42664.0
58	39508.0
59	34739.0
60	29970.0
61	29970.0
62	26938.5
63	23907.0
64	20604.0
65	17301.0
66	14519.5
67	11738.0
68	11738.0
69	9671.5
70	7605.0
71	6648.0
72	5691.0
73	4541.0
74	3391.0
75	3391.0
76	2644.5
77	1898.0
78	1663.0
79	1428.0
80	1002.0
81	576.0
82	576.0
83	436.0
84	296.0
85	223.0
86	150.0
87	113.5
88	77.0
89	77.0
90	60.0
91	43.0
92	29.0
93	15.0
94	10.0
95	5.0
96	5.0
97	4.5
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	679066.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.18474421829143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.64250586576313	49.136333328713114
2	11.496143062030901	13.83784859370431
3	3.2251684178496896	5.823178088675856
4	1.256721083621268	3.0254174788592025
5	0.6108102826578343	1.8380730313832023
6	0.3587197812396195	1.2953674967968776
7	0.2303612506742754	0.9704963064745888
8	0.1578129393466926	0.7598345111133947
9	0.13454898521858508	0.7288016634190096
>10	0.7161148239238612	8.292652541543525
>50	0.09790906341528953	4.257722664976335
>100	0.07071199681771631	7.997152779818095
>500	0.0014834684646897916	0.4880758632311361
>1k	9.88978976459861E-4	1.5490456512915018
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3552	0.5230713951221236	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3027	0.4457593223633638	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2338	0.3442964306856771	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1493	0.21986080881681602	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.4726109096906633E-4	0.0
11	0.0	0.0	0.0	1.4726109096906633E-4	0.0
12	0.0	0.0	0.0	1.4726109096906633E-4	0.0
13	0.0	0.0	0.0	1.4726109096906633E-4	0.0
14	0.0	0.0	0.0	1.4726109096906633E-4	0.0
15	0.0	0.0	0.0	1.4726109096906633E-4	0.0
16	0.0	0.0	0.0	1.4726109096906633E-4	0.0
17	0.0	0.0	0.0	1.4726109096906633E-4	0.0
18	0.0	0.0	0.0	1.4726109096906633E-4	0.0
19	0.0	0.0	0.0	2.9452218193813266E-4	0.0
20	0.0	0.0	0.0	7.363054548453317E-4	0.0
21	0.0	0.0	0.0	7.363054548453317E-4	0.0
22	0.0	0.0	0.0	0.0010308276367834643	0.0
23	0.0	0.0	0.0	0.0029452218193813267	0.0
24	0.0	0.0	0.0	0.006921271275546118	0.0
25	0.0	0.0	0.0	0.013989803642061302	0.0
26	0.0	0.0	0.0	0.025770690919586607	0.0
27	0.0	0.0	0.0	0.10028480294993418	0.0
28	0.0	0.0	0.0	0.21986080881681605	0.0
29	0.0	0.0	0.0	0.3425292975940483	0.0
30	0.0	0.0	0.0	0.4759478460120224	0.0
31	0.0	0.0	0.0	0.6996374431940342	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	850	0.0	25.464706	1
GCATAGA	60	3.7233953E-5	21.583334	1
TAACGTA	45	0.003824221	20.555555	32
AACGTAA	55	5.140114E-4	20.181818	33
ATAGGTG	75	9.256615E-6	19.733334	5
TACACTG	150	0.0	19.733334	5
ACCGCGC	60	9.231508E-4	18.5	18
TGTATAC	60	9.231508E-4	18.5	3
TTAACGT	60	9.231508E-4	18.5	31
AGCGTTA	50	0.007031992	18.499998	19
CTAGACT	50	0.007031992	18.499998	4
ACTTCGT	50	0.007031992	18.499998	8
CGTCAGA	65	0.0015790337	17.076923	1
GTGCTAG	175	7.2759576E-12	16.914286	1
GTATCAA	1315	0.0	16.460075	2
GTATTAG	125	1.6546619E-7	16.28	1
TGCTCGG	80	3.380017E-4	16.1875	15
TCTATAC	80	3.380017E-4	16.1875	3
TCGCCAT	150	4.658432E-9	16.033333	13
TAGACTT	70	0.0025910286	15.857143	5
>>END_MODULE