FastQCFastQC Report
Fri 10 Feb 2017
ERR1630074.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630074.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17260
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4152.4044032444959442No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3722.1552723059096177No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2941.7033603707995364No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1761.0196987253765932No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.27809965237543455No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.26651216685979146No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.2607184241019699No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.2549246813441483No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT390.22595596755504055TruSeq Adapter, Index 10 (95% over 21bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.220162224797219No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT360.20857473928157588No Hit
GTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.20278099652375434No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.19119351100811124No Hit
TGCCTGTACTGCCTCCCCCGTACTCTGCGTTGATACCCTGTCT300.17381228273464658No Hit
GAGTACGGGGGAGGCAGTACAGGCACTAAAAAAAAAAAAAAAA290.16801853997682503No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG270.1564310544611819No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.1564310544611819No Hit
AGGCAGTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAA240.13904982618771727No Hit
GTACGGGGGAGGCAGTACAGGCACTAAAAAAAAAAAAAAAAAA180.10428736964078794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1454.3788532E-815.3103451
GTATCAA2750.013.4545461
GTCTCTT1102.2441057E-413.45454537
AACGCAG3359.094947E-1211.0447766
CGCAGAG3359.094947E-1211.0447768
GCAGAGT3451.6370905E-1110.7246379
CCTGTCT1250.00906261410.36000136
CAGAGTA3451.8553692E-1010.18840510
AGAGTAC3451.8553692E-1010.18840511
ACGCAGA3654.5474735E-1110.1369877
ATCAACG3706.002665E-1110.03
CAACGCA3757.8216544E-119.8666665
TATCAAC3801.0004442E-109.7368422
TCAACGC3851.2914825E-109.610394
CTGTCTC2003.161982E-49.24999937
TGTCTCT1650.006656688.96969736
AGTACGG1700.0085058538.7058822
GAGTACG1700.0085058538.7058821
AGTACTT2402.0206954E-48.47916713
GAGTACT2452.5266391E-48.30612212
Produced by FastQC (version 0.11.5)