##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630074.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17260
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50139049826188	33.0	31.0	34.0	31.0	34.0
2	32.69101969872538	34.0	31.0	34.0	31.0	34.0
3	32.77601390498262	34.0	31.0	34.0	31.0	34.0
4	36.116801853997686	37.0	35.0	37.0	35.0	37.0
5	36.13910776361529	37.0	35.0	37.0	35.0	37.0
6	36.23464658169177	37.0	37.0	37.0	35.0	37.0
7	36.13899188876014	37.0	36.0	37.0	35.0	37.0
8	36.22833140208575	37.0	37.0	37.0	35.0	37.0
9	37.91442641946698	39.0	38.0	39.0	35.0	39.0
10	37.8824449594438	39.0	38.0	39.0	35.0	39.0
11	37.940845886442645	39.0	38.0	39.0	35.0	39.0
12	37.84675550405562	39.0	38.0	39.0	35.0	39.0
13	37.9005214368482	39.0	38.0	39.0	35.0	39.0
14	39.09971031286211	40.0	38.0	41.0	36.0	41.0
15	39.092004634994204	40.0	39.0	41.0	36.0	41.0
16	38.94119351100811	40.0	38.0	41.0	35.0	41.0
17	39.03418308227115	40.0	38.0	41.0	36.0	41.0
18	38.98580533024334	40.0	39.0	41.0	36.0	41.0
19	39.07578215527231	40.0	39.0	41.0	35.0	41.0
20	39.210602549246815	41.0	39.0	41.0	36.0	41.0
21	39.15527230590962	41.0	39.0	41.0	35.0	41.0
22	39.155561993047506	41.0	39.0	41.0	35.0	41.0
23	39.00173812282735	41.0	39.0	41.0	35.0	41.0
24	38.91234067207416	41.0	38.0	41.0	35.0	41.0
25	38.86042873696408	41.0	38.0	41.0	35.0	41.0
26	38.55921205098494	40.0	38.0	41.0	35.0	41.0
27	38.320683661645425	40.0	37.0	41.0	34.0	41.0
28	38.20139049826188	40.0	37.0	41.0	34.0	41.0
29	37.96205098493627	40.0	37.0	41.0	34.0	41.0
30	37.63539976825029	40.0	36.0	41.0	33.0	41.0
31	37.32723059096176	39.0	35.0	41.0	33.0	41.0
32	37.06205098493627	39.0	35.0	41.0	33.0	41.0
33	36.77537659327926	39.0	35.0	41.0	32.0	41.0
34	36.53337195828505	39.0	35.0	41.0	32.0	41.0
35	36.24409038238702	39.0	35.0	41.0	31.0	41.0
36	35.925144843568944	38.0	35.0	41.0	30.0	41.0
37	35.66112398609502	38.0	35.0	41.0	28.0	41.0
38	35.33453070683662	38.0	35.0	41.0	26.0	41.0
39	35.06419466975666	38.0	35.0	41.0	24.0	41.0
40	34.71257242178447	38.0	35.0	41.0	22.0	41.0
41	34.25915411355736	38.0	35.0	40.0	18.0	41.0
42	33.77786790266512	37.0	34.0	40.0	15.0	41.0
43	33.18887601390498	37.0	33.0	40.0	11.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	1.0
20	4.0
21	6.0
22	24.0
23	33.0
24	34.0
25	57.0
26	113.0
27	150.0
28	191.0
29	267.0
30	285.0
31	361.0
32	468.0
33	582.0
34	747.0
35	995.0
36	1444.0
37	2759.0
38	3182.0
39	5554.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.13441483198146	17.995365005793744	14.58285052143685	19.28736964078795
2	21.04287369640788	24.409038238702202	32.21320973348784	22.334878331402088
3	22.42757821552723	24.466975666280415	32.30011587485516	20.805330243337195
4	13.835457705677868	17.39860950173812	32.46813441483199	36.29779837775203
5	13.684820393974508	38.516801853997684	32.549246813441485	15.249130938586328
6	31.819235225955968	34.42062572421784	14.044032444959445	19.71610660486674
7	26.129779837775203	32.37543453070684	20.834298957126304	20.660486674391656
8	29.194669756662805	31.813441483198147	18.267670915411355	20.724217844727693
9	25.44032444959444	14.293163383545771	19.942062572421783	40.32444959443801
10	20.730011587485517	28.070683661645425	27.566628041714946	23.63267670915411
11	33.69061413673233	22.902665121668598	20.59675550405562	22.80996523754345
12	22.15527230590962	30.318655851680187	27.230590961761298	20.2954808806489
13	32.83314020857474	20.44032444959444	24.565469293163382	22.16106604866744
14	20.93858632676709	24.490150637311704	28.84704519119351	25.724217844727693
15	25.359212050984937	28.006952491309384	25.353418308227116	21.280417149478563
16	20.336037079953652	26.946697566628043	28.66743916570104	24.049826187717265
17	20.74739281575898	29.032444959443797	27.87369640787949	22.34646581691773
18	20.96176129779838	24.97103128621089	29.80301274623407	24.264194669756662
19	23.638470451911935	26.129779837775203	31.44843568945539	18.783314020857475
20	23.27346465816918	25.770567786790266	30.9675550405562	19.98841251448436
21	22.375434530706837	26.46581691772885	31.825028968713788	19.333719582850524
22	21.3441483198146	27.566628041714946	31.37311703360371	19.71610660486674
23	20.689455388180765	27.12050984936269	31.819235225955968	20.37079953650058
24	20.214368482039397	27.54345307068366	32.004634994206256	20.237543453070682
25	20.30127462340672	27.12050984936269	31.714947856315177	20.863267670915413
26	20.388180764774045	27.94322132097335	31.911935110081114	19.756662804171494
27	19.73348783314021	28.638470451911935	32.560834298957126	19.06720741599073
28	19.061413673232906	28.9513325608343	32.7694090382387	19.217844727694093
29	19.959443800695247	28.12862108922364	32.34067207415991	19.571263035921206
30	19.356894553881805	29.049826187717265	32.862108922363845	18.73117033603708
31	18.77172653534183	29.611819235225955	32.68250289687138	18.933951332560834
32	18.870220162224797	29.565469293163382	32.08574739281576	19.47856315179606
33	18.870220162224797	29.51911935110081	32.050984936268826	19.55967555040556
34	18.592120509849362	29.020857473928157	32.14368482039397	20.243337195828506
35	18.487833140208576	29.25260718424102	32.97798377752027	19.28157589803013
36	19.165701042873696	29.29895712630359	31.286210892236383	20.249130938586326
37	17.850521436848204	28.980301274623404	32.236384704519125	20.93279258400927
38	18.174971031286212	28.076477404403242	32.94322132097335	20.805330243337195
39	18.203939745075317	28.806488991888763	33.00695249130939	19.982618771726536
40	17.38702201622248	28.783314020857475	33.60950173812283	20.22016222479722
41	17.64774044032445	28.9513325608343	33.215527230590965	20.18539976825029
42	16.703360370799537	28.93974507531866	32.88528389339513	21.471610660486675
43	16.888760139049825	28.198146002317497	33.83545770567787	21.07763615295481
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	67.0
1	56.0
2	45.0
3	63.5
4	82.0
5	82.0
6	102.0
7	122.0
8	138.5
9	155.0
10	189.0
11	223.0
12	223.0
13	375.5
14	528.0
15	731.0
16	934.0
17	823.5
18	713.0
19	713.0
20	747.0
21	781.0
22	547.0
23	313.0
24	267.5
25	222.0
26	222.0
27	233.5
28	245.0
29	276.5
30	308.0
31	342.5
32	377.0
33	377.0
34	397.5
35	418.0
36	478.0
37	538.0
38	560.0
39	582.0
40	582.0
41	598.5
42	615.0
43	670.0
44	725.0
45	775.5
46	826.0
47	826.0
48	924.5
49	1023.0
50	1041.5
51	1060.0
52	1032.0
53	1004.0
54	1004.0
55	1020.5
56	1037.0
57	1009.5
58	982.0
59	916.5
60	851.0
61	851.0
62	774.0
63	697.0
64	619.5
65	542.0
66	483.0
67	424.0
68	424.0
69	366.0
70	308.0
71	257.5
72	207.0
73	169.5
74	132.0
75	132.0
76	101.0
77	70.0
78	59.5
79	49.0
80	40.5
81	32.0
82	32.0
83	22.0
84	12.0
85	10.5
86	9.0
87	5.5
88	2.0
89	2.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	17260.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.55735805330242
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.00531012611549	72.27694090382387
2	5.590382771590825	8.783314020857475
3	1.0398996976178183	2.450753186558517
4	0.4351353344641935	1.3673232908458863
5	0.21388007965189174	0.8400926998841253
6	0.13275315288738107	0.6257242178447276
7	0.14012832804779113	0.7705677867902665
8	0.0737517516041006	0.4634994206257242
9	0.0368758758020503	0.2607184241019699
>10	0.30238218157681246	4.878331402085748
>50	0.0	0.0
>100	0.029500700641640235	7.282734646581692
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	415	2.4044032444959442	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	372	2.1552723059096177	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	294	1.7033603707995364	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	176	1.0196987253765932	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48	0.27809965237543455	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46	0.26651216685979146	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45	0.2607184241019699	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44	0.2549246813441483	No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT	39	0.22595596755504055	TruSeq Adapter, Index 10 (95% over 21bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38	0.220162224797219	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT	36	0.20857473928157588	No Hit
GTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35	0.20278099652375434	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	33	0.19119351100811124	No Hit
TGCCTGTACTGCCTCCCCCGTACTCTGCGTTGATACCCTGTCT	30	0.17381228273464658	No Hit
GAGTACGGGGGAGGCAGTACAGGCACTAAAAAAAAAAAAAAAA	29	0.16801853997682503	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	27	0.1564310544611819	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27	0.1564310544611819	No Hit
AGGCAGTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAA	24	0.13904982618771727	No Hit
GTACGGGGGAGGCAGTACAGGCACTAAAAAAAAAAAAAAAAAA	18	0.10428736964078794	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.005793742757821553	0.0	0.0	0.0	0.0
11	0.005793742757821553	0.0	0.0	0.0	0.0
12	0.005793742757821553	0.0	0.0	0.0	0.0
13	0.005793742757821553	0.0	0.0	0.0	0.0
14	0.005793742757821553	0.0	0.0	0.0	0.0
15	0.005793742757821553	0.0	0.0	0.0	0.0
16	0.005793742757821553	0.0	0.0	0.0	0.0
17	0.005793742757821553	0.0	0.0	0.0	0.0
18	0.005793742757821553	0.0	0.0	0.0	0.0
19	0.011587485515643106	0.0	0.0	0.0	0.0
20	0.011587485515643106	0.0	0.0	0.0	0.0
21	0.011587485515643106	0.0	0.0	0.0	0.0
22	0.011587485515643106	0.0	0.0	0.0	0.0
23	0.011587485515643106	0.0	0.0	0.005793742757821553	0.0
24	0.011587485515643106	0.0	0.0	0.011587485515643106	0.0
25	0.011587485515643106	0.0	0.0	0.03476245654692932	0.0
26	0.011587485515643106	0.0	0.0	0.05793742757821553	0.0
27	0.011587485515643106	0.0	0.0	0.1564310544611819	0.0
28	0.011587485515643106	0.0	0.0	0.388180764774044	0.0
29	0.011587485515643106	0.0	0.0	0.59675550405562	0.0
30	0.011587485515643106	0.0	0.0	0.93279258400927	0.0
31	0.011587485515643106	0.0	0.0	1.2977983777520279	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	145	4.3788532E-8	15.310345	1
GTATCAA	275	0.0	13.454546	1
GTCTCTT	110	2.2441057E-4	13.454545	37
AACGCAG	335	9.094947E-12	11.044776	6
CGCAGAG	335	9.094947E-12	11.044776	8
GCAGAGT	345	1.6370905E-11	10.724637	9
CCTGTCT	125	0.009062614	10.360001	36
CAGAGTA	345	1.8553692E-10	10.188405	10
AGAGTAC	345	1.8553692E-10	10.188405	11
ACGCAGA	365	4.5474735E-11	10.136987	7
ATCAACG	370	6.002665E-11	10.0	3
CAACGCA	375	7.8216544E-11	9.866666	5
TATCAAC	380	1.0004442E-10	9.736842	2
TCAACGC	385	1.2914825E-10	9.61039	4
CTGTCTC	200	3.161982E-4	9.249999	37
TGTCTCT	165	0.00665668	8.969697	36
AGTACGG	170	0.008505853	8.705882	2
GAGTACG	170	0.008505853	8.705882	1
AGTACTT	240	2.0206954E-4	8.479167	13
GAGTACT	245	2.5266391E-4	8.306122	12
>>END_MODULE