FastQCFastQC Report
Fri 10 Feb 2017
ERR1630073.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630073.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2954562
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT96380.3262074040077683No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT84060.28450917597938374No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT65630.22213106375835065No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47090.15938064592992124No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC36400.12319931008386352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25200.025.841271
TATACCG752.0686517E-417.2666665
GTATCAA38750.016.7096772
ATAACGC908.2794356E-414.3888893
TATACTG4800.013.4895835
TCTTGCG3450.013.4057967
CGCGCTT1409.5701635E-613.21428512
CGAATTA4200.013.21428515
ATAGTAC4200.012.7738093
CGCGAAC1451.3735891E-512.7586228
TCGTAGG1451.3735891E-512.758622
GTATAGG3700.012.51
ATCAACG51950.012.3926854
TATCAAC53050.012.3449573
TTATACC4350.012.3333344
CCGAACA1359.970296E-512.3333339
TATAACG1050.002645264612.3333332
CAGGACA19000.012.2684214
CTTATAC21000.012.24523737
TCAACGC52950.012.158645
Produced by FastQC (version 0.11.5)