FastQCFastQC Report
Fri 10 Feb 2017
ERR1630072.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630072.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487888
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13600.2787525005739022No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10900.22341193060702458No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8910.18262388089069623No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6470.13261240284655496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTA551.8511855E-830.27272815
TCGTAGT250.00549328129.64
CTAGGAC551.8997504E-523.5454543
GGTATCA3750.023.1866651
ACTAGAC400.001929603223.1253
GCGCTTT400.001929603223.1256
CGTCAGA502.699014E-422.21
CCCCGAA705.0919953E-621.14285912
CTAGACA609.226431E-418.54
CTATACT701.21737256E-418.54
CCGAATT801.6135647E-518.514
TCTATAC902.1470532E-618.53
GTATCAA4700.018.1063842
TTCTATA1054.7832873E-717.6190472
GCCCCGA1001.0913159E-414.811
CCCGAAT1001.0913159E-414.813
TACAGAG1651.6369086E-814.5757595
GTCCTAA1152.204583E-514.4782611
TAGTCCT1803.3105607E-914.3888884
GTGTAGG1458.878924E-714.0344831
Produced by FastQC (version 0.11.5)