##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630072.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	487888
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.85380661135343	34.0	31.0	34.0	31.0	34.0
2	33.08309694028138	34.0	33.0	34.0	31.0	34.0
3	33.207305775095925	34.0	33.0	34.0	31.0	34.0
4	36.49568138589184	37.0	37.0	37.0	35.0	37.0
5	36.43670678516381	37.0	37.0	37.0	35.0	37.0
6	36.48135022792116	37.0	37.0	37.0	35.0	37.0
7	36.40452726855344	37.0	37.0	37.0	35.0	37.0
8	36.409151280621785	37.0	37.0	37.0	35.0	37.0
9	38.18640753615584	39.0	39.0	39.0	37.0	39.0
10	38.154824467910665	39.0	39.0	39.0	37.0	39.0
11	38.19141893221395	39.0	39.0	39.0	37.0	39.0
12	38.12855204473158	39.0	39.0	39.0	37.0	39.0
13	38.14825533729053	39.0	39.0	39.0	37.0	39.0
14	39.60798379956056	41.0	40.0	41.0	37.0	41.0
15	39.587440150198404	41.0	40.0	41.0	37.0	41.0
16	39.50477978552455	41.0	39.0	41.0	37.0	41.0
17	39.42135900042633	41.0	39.0	41.0	37.0	41.0
18	39.31478331092382	41.0	39.0	41.0	36.0	41.0
19	39.33534335749189	41.0	39.0	41.0	36.0	41.0
20	39.56514404945397	41.0	39.0	41.0	37.0	41.0
21	39.578372495326796	41.0	40.0	41.0	37.0	41.0
22	39.59695052962975	41.0	40.0	41.0	37.0	41.0
23	39.53134940806087	41.0	40.0	41.0	37.0	41.0
24	39.49584125864953	41.0	40.0	41.0	37.0	41.0
25	39.46522152625192	41.0	40.0	41.0	37.0	41.0
26	39.32359680910373	41.0	39.0	41.0	36.0	41.0
27	39.246105663594925	41.0	39.0	41.0	36.0	41.0
28	39.2049650739514	41.0	39.0	41.0	36.0	41.0
29	39.09769045354672	41.0	39.0	41.0	36.0	41.0
30	39.00747097694553	40.0	39.0	41.0	35.0	41.0
31	38.88227830977601	40.0	39.0	41.0	35.0	41.0
32	38.7910565539632	40.0	39.0	41.0	35.0	41.0
33	38.72549642540911	40.0	38.0	41.0	35.0	41.0
34	38.648892778670515	40.0	38.0	41.0	35.0	41.0
35	38.524940150198404	40.0	38.0	41.0	35.0	41.0
36	38.51439264749287	40.0	38.0	41.0	35.0	41.0
37	38.47968386186994	40.0	38.0	41.0	35.0	41.0
38	38.39192601580691	40.0	38.0	41.0	35.0	41.0
39	38.33973165972518	40.0	38.0	41.0	34.0	41.0
40	38.27340701144525	40.0	38.0	41.0	34.0	41.0
41	38.185218738726924	40.0	38.0	41.0	34.0	41.0
42	38.095284163578526	40.0	38.0	41.0	34.0	41.0
43	37.79895180861181	40.0	38.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	1.0
15	2.0
16	2.0
17	5.0
18	19.0
19	33.0
20	60.0
21	108.0
22	202.0
23	308.0
24	519.0
25	768.0
26	1147.0
27	1537.0
28	2075.0
29	2805.0
30	3745.0
31	4833.0
32	6025.0
33	8158.0
34	10808.0
35	15491.0
36	23571.0
37	41078.0
38	89052.0
39	275534.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.220165283835634	19.181451480667693	12.069778309776014	25.528604925720654
2	18.920121011379663	21.221468861705965	35.07444331485915	24.783966812055226
3	19.014199980323355	22.95424359689109	31.134604663365362	26.896951759420197
4	14.260240055094611	15.77226740563408	35.724387564359034	34.24310497491228
5	14.195676056799922	36.49259666152888	35.1037533204342	14.207973961237006
6	34.13590824123569	35.7717344964418	15.39554159971141	14.69681566261109
7	28.20913816285705	30.843349293280426	22.243834650575543	18.703677893286986
8	26.250901846325387	34.27774407241006	20.428254025514054	19.0431000557505
9	25.765954481356378	14.604171449185058	20.507985439281146	39.12188863017742
10	16.29369855376644	27.14864887023251	34.75879710097399	21.798855475027054
11	34.52636670711311	22.237685698356998	22.483438822024727	20.752508772505166
12	20.330690650313187	25.545002131636767	31.14485291706293	22.97945430098711
13	29.442413012822616	20.841668579674025	25.32445971206506	24.3914586954383
14	21.520512904601055	21.536500180369263	26.353384383301087	30.589602531728595
15	24.05306135834454	28.566802216902236	23.3590496179451	24.02108680680812
16	23.703800872331353	27.511027120978586	24.946299150624736	23.838872856065326
17	22.62896402453022	27.349719607778834	26.862107696848458	23.15920867084249
18	22.87205260223658	26.467344964418064	27.454661725641948	23.205940707703405
19	23.61197651920113	26.111525596038437	27.67991834191454	22.596579542845898
20	23.711179614993604	26.056389991145508	27.415718361591185	22.816712032269702
21	23.31785163808087	26.377365296953396	27.054774866362774	23.25000819860296
22	23.378726265044435	26.244752894106842	27.17713901551176	23.199381825336964
23	23.53306496572984	26.291894861115665	26.84366084019283	23.331379332961664
24	23.513388318630504	26.118904338700688	26.996154855212673	23.37155248745614
25	23.585126094513495	25.962925917423675	27.15110845111993	23.300839536942906
26	23.37155248745614	26.491325878070377	26.886498540648674	23.250623093824814
27	23.276448693142687	26.54933099399862	26.765364181943397	23.408856130915293
28	23.237915259239823	26.535188403895976	26.86354245236612	23.363353884498082
29	23.133383071524612	26.46017118682976	27.398091365231362	23.008354376414257
30	23.310062965270717	26.614304922441217	26.984471845997444	23.091160266290625
31	23.321336044338047	26.243728068737088	27.163611320630963	23.271324566293902
32	22.87615190371561	26.32284458728233	27.434165218246807	23.366838290755254
33	22.83392909848162	26.31567080969403	27.43519004361657	23.415210048207786
34	23.261281277670285	25.872946249959007	27.19476601187158	23.67100646049913
35	23.02577640770013	25.823344702062766	27.759854720755584	23.39102416948152
36	23.05385662283147	25.532909192273635	27.728495064441017	23.684739120453873
37	22.9267782769816	24.75998589840291	28.318179582199193	23.995056242416293
38	22.62629947856885	25.214598432427117	28.55983340438789	23.599268684616142
39	22.25838717082609	25.27547305939068	28.9422982323812	23.523841537402028
40	22.0981044829961	24.747483028891875	29.44999672055882	23.704415767553208
41	21.71461482963303	24.84320171842718	29.377234119306074	24.06494933263372
42	21.533425704259994	24.928672154264913	29.295658019873414	24.24224412160168
43	21.277014396746793	24.825984652215265	29.635695077558783	24.26130587347916
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	450.0
1	354.0
2	258.0
3	453.5
4	649.0
5	649.0
6	705.0
7	761.0
8	655.0
9	549.0
10	767.0
11	985.0
12	985.0
13	1499.5
14	2014.0
15	2548.5
16	3083.0
17	3385.0
18	3687.0
19	3687.0
20	4817.0
21	5947.0
22	6323.5
23	6700.0
24	8049.0
25	9398.0
26	9398.0
27	10518.0
28	11638.0
29	13033.0
30	14428.0
31	16118.0
32	17808.0
33	17808.0
34	19457.0
35	21106.0
36	22814.0
37	24522.0
38	25799.0
39	27076.0
40	27076.0
41	28179.0
42	29282.0
43	29495.5
44	29709.0
45	30193.5
46	30678.0
47	30678.0
48	30751.0
49	30824.0
50	31473.0
51	32122.0
52	32448.0
53	32774.0
54	32774.0
55	31520.0
56	30266.0
57	29098.0
58	27930.0
59	25931.0
60	23932.0
61	23932.0
62	22055.0
63	20178.0
64	17912.0
65	15646.0
66	13762.5
67	11879.0
68	11879.0
69	9953.0
70	8027.0
71	6774.0
72	5521.0
73	4480.5
74	3440.0
75	3440.0
76	2784.0
77	2128.0
78	1700.5
79	1273.0
80	945.0
81	617.0
82	617.0
83	454.5
84	292.0
85	226.0
86	160.0
87	123.5
88	87.0
89	87.0
90	65.0
91	43.0
92	28.5
93	14.0
94	8.5
95	3.0
96	3.0
97	3.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	487888.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.11542557561023
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.83594425729319	70.17573017645256
2	6.546231486081461	10.227233169034253
3	1.5786035559012972	3.699398657532043
4	0.6459374085811667	2.0183070226609865
5	0.3367291064106613	1.3151868725481874
6	0.21822733577910558	1.0228152723969854
7	0.15069895584292978	0.824033914862939
8	0.1057378412885202	0.6607805177359268
9	0.08893335605188582	0.62523602602743
>10	0.4615578275427719	6.4125582665343535
>50	0.023219332956370495	1.217505202785538
>100	0.007124112235747591	0.9792392718490038
>500	5.277120174627598E-4	0.3170006314678438
>1k	5.277120174627598E-4	0.5049749981119749
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1360	0.2787525005739022	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1090	0.22341193060702458	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	891	0.18262388089069623	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	647	0.13261240284655496	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	2.0496507395139868E-4	0.0
13	0.0	0.0	0.0	2.0496507395139868E-4	0.0
14	0.0	0.0	0.0	2.0496507395139868E-4	0.0
15	0.0	0.0	0.0	2.0496507395139868E-4	0.0
16	0.0	0.0	0.0	4.0993014790279735E-4	0.0
17	0.0	0.0	0.0	4.0993014790279735E-4	0.0
18	0.0	0.0	0.0	4.0993014790279735E-4	0.0
19	0.0	0.0	0.0	4.0993014790279735E-4	0.0
20	0.0	0.0	0.0	4.0993014790279735E-4	0.0
21	0.0	0.0	0.0	8.198602958055947E-4	0.0
22	0.0	0.0	0.0	0.001844685665562588	0.0
23	0.0	4.0993014790279735E-4	0.0	0.0030744761092709803	0.0
24	0.0	4.0993014790279735E-4	0.0	0.006763847440396156	0.0
25	0.0	4.0993014790279735E-4	0.0	0.011478044141278326	0.0
26	0.0	4.0993014790279735E-4	0.0	0.01988161217328567	0.0
27	0.0	4.0993014790279735E-4	0.0	0.0785016233233857	0.0
28	0.0	4.0993014790279735E-4	0.0	0.18467353163021022	0.0
29	0.0	6.14895221854196E-4	0.0	0.2990440428950907	0.0
30	0.0	6.14895221854196E-4	0.0	0.41874364608270753	0.0
31	0.0	6.14895221854196E-4	0.0	0.6757698488177615	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATTA	55	1.8511855E-8	30.272728	15
TCGTAGT	25	0.005493281	29.6	4
CTAGGAC	55	1.8997504E-5	23.545454	3
GGTATCA	375	0.0	23.186665	1
ACTAGAC	40	0.0019296032	23.125	3
GCGCTTT	40	0.0019296032	23.125	6
CGTCAGA	50	2.699014E-4	22.2	1
CCCCGAA	70	5.0919953E-6	21.142859	12
CTAGACA	60	9.226431E-4	18.5	4
CTATACT	70	1.21737256E-4	18.5	4
CCGAATT	80	1.6135647E-5	18.5	14
TCTATAC	90	2.1470532E-6	18.5	3
GTATCAA	470	0.0	18.106384	2
TTCTATA	105	4.7832873E-7	17.619047	2
GCCCCGA	100	1.0913159E-4	14.8	11
CCCGAAT	100	1.0913159E-4	14.8	13
TACAGAG	165	1.6369086E-8	14.575759	5
GTCCTAA	115	2.204583E-5	14.478261	1
TAGTCCT	180	3.3105607E-9	14.388888	4
GTGTAGG	145	8.878924E-7	14.034483	1
>>END_MODULE