FastQCFastQC Report
Fri 10 Feb 2017
ERR1630071.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630071.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1927300
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT51480.26710942769677787No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42980.22300627821304417No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33450.17355886473304624No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24930.1293519431328802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12550.026.8286861
ACGTTAC400.00193165923.12526
TTTACGC759.271109E-619.73333433
CGCTTAC500.00703656718.51
GTATCAA18700.017.9064162
CGAATTA1708.54925E-1116.3235315
CGTGCGA803.3840514E-416.187510
TAACGGC700.002593346915.85714336
TAGGGGT2400.015.4166664
TACGCCG1101.4528034E-515.13636435
GTATAGG2600.014.9423071
CGTTACT750.00410574614.827
TATCTCG750.00410574614.85
TTAACGG750.00410574614.835
CGCCGAT1252.9610765E-614.79999937
TCGAATA908.277599E-414.38888813
TTACGCC1051.6565487E-414.09523834
CGAGCGT800.006300661213.8756
TCAACGC24600.013.6117895
AACTCGA1102.4577128E-413.45454526
Produced by FastQC (version 0.11.5)