FastQCFastQC Report
Fri 10 Feb 2017
ERR1630070.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630070.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2761532
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT81970.2968279925780328No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT74260.26890870719586085No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59810.21658267946922213No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC53320.19308123172210206No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42580.15418977582008828No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT27830.10077739457663355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20400.023.4877451
CGAATTA2000.016.65000215
GTATCAA32150.014.9035782
GTACTAG1252.9622006E-614.7999991
CCGAATT2500.014.05999914
GACAGGC17900.014.0558667
CGTGCGA1203.3036566E-513.87499910
GTACGTA1203.3036566E-513.87499913
TGCATCA16850.013.72403614
CGACGTT1102.4583176E-413.45454524
CAGGACA21150.013.3829794
ATTAGAG4300.013.3372093
GGACAGG19850.013.2342576
AGACGTA1703.7366044E-713.0588245
TACGTAG850.00940909913.05882414
GCTGCAT17950.012.98607312
TGCACCG7000.012.9500015
GCATCAG18200.012.90934215
ACAGGCT20250.012.6074088
TAACGGC2358.094503E-1012.59574536
Produced by FastQC (version 0.11.5)