##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630070.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2761532
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83854469185945	34.0	31.0	34.0	31.0	34.0
2	33.06624257839489	34.0	33.0	34.0	31.0	34.0
3	33.18233393638024	34.0	33.0	34.0	31.0	34.0
4	36.47895769449711	37.0	37.0	37.0	35.0	37.0
5	36.426967712125006	37.0	37.0	37.0	35.0	37.0
6	36.47303598147695	37.0	37.0	37.0	35.0	37.0
7	36.39846469278647	37.0	37.0	37.0	35.0	37.0
8	36.40543618542171	37.0	37.0	37.0	35.0	37.0
9	38.18066855643896	39.0	39.0	39.0	37.0	39.0
10	38.154068104226205	39.0	39.0	39.0	37.0	39.0
11	38.18849609564546	39.0	39.0	39.0	37.0	39.0
12	38.122097444461986	39.0	39.0	39.0	37.0	39.0
13	38.12991882766522	39.0	39.0	39.0	37.0	39.0
14	39.587742962964036	41.0	40.0	41.0	37.0	41.0
15	39.57308298437244	41.0	40.0	41.0	37.0	41.0
16	39.49511973788462	41.0	39.0	41.0	37.0	41.0
17	39.416788217554604	41.0	39.0	41.0	37.0	41.0
18	39.27801597084517	41.0	39.0	41.0	36.0	41.0
19	39.3098993602102	41.0	39.0	41.0	36.0	41.0
20	39.53064096305963	41.0	39.0	41.0	37.0	41.0
21	39.547980975777214	41.0	40.0	41.0	37.0	41.0
22	39.56406914712558	41.0	40.0	41.0	37.0	41.0
23	39.498206430343735	41.0	40.0	41.0	37.0	41.0
24	39.45362537895632	41.0	40.0	41.0	36.0	41.0
25	39.42196758900494	41.0	39.0	41.0	36.0	41.0
26	39.26631992676529	41.0	39.0	41.0	36.0	41.0
27	39.188982419903155	41.0	39.0	41.0	36.0	41.0
28	39.13148172825808	41.0	39.0	41.0	36.0	41.0
29	39.0245530379514	41.0	39.0	41.0	35.0	41.0
30	38.93178568997209	40.0	39.0	41.0	35.0	41.0
31	38.816693777222206	40.0	39.0	41.0	35.0	41.0
32	38.727149278009456	40.0	39.0	41.0	35.0	41.0
33	38.648869902648244	40.0	38.0	41.0	35.0	41.0
34	38.57262744013106	40.0	38.0	41.0	35.0	41.0
35	38.426488268106255	40.0	38.0	41.0	35.0	41.0
36	38.4138289905748	40.0	38.0	41.0	35.0	41.0
37	38.354799437413725	40.0	38.0	41.0	34.0	41.0
38	38.25897871181648	40.0	38.0	41.0	34.0	41.0
39	38.211030326644774	40.0	38.0	41.0	34.0	41.0
40	38.13044969241711	40.0	38.0	41.0	34.0	41.0
41	38.020454950368126	40.0	38.0	41.0	34.0	41.0
42	37.92564634413072	40.0	38.0	41.0	33.0	41.0
43	37.63152880357715	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	5.0
13	6.0
14	5.0
15	11.0
16	16.0
17	46.0
18	102.0
19	203.0
20	418.0
21	731.0
22	1347.0
23	2121.0
24	3284.0
25	4892.0
26	6960.0
27	9550.0
28	13004.0
29	17315.0
30	22026.0
31	28241.0
32	36379.0
33	47289.0
34	65278.0
35	90872.0
36	134544.0
37	234368.0
38	505460.0
39	1537057.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.661091017594586	18.457037615352636	11.789760176597627	26.092111190455153
2	19.20984439072225	20.440357019219764	35.001513652566764	25.348284937491215
3	19.369791840181463	22.877663557764315	30.18469458257228	27.567850019481938
4	14.406170198281243	15.553106029551714	35.125032047428746	34.915691724738295
5	14.520527011818077	36.41304898874972	34.50443449505564	14.561989504376557
6	34.79651874394358	35.17862548759167	15.222275171897337	14.802580596567413
7	29.081285315542242	29.99259106901532	21.904978830591133	19.02114478485131
8	26.764926135203215	34.081734341662525	19.786589472799882	19.36675005033438
9	26.322309500668467	14.252559811003456	19.701709051352655	39.72342163697542
10	16.881752592401607	26.657123654551167	33.7960957903077	22.665027962739522
11	35.007090267286415	21.960020742109815	21.931847974240384	21.101041016363382
12	21.441431784965737	25.08473557431165	30.16380038326552	23.31003225745709
13	29.83057954787415	20.133606997854812	24.97555704587164	25.060256408399397
14	22.0362827589903	20.919294073000056	26.10221427816154	30.9422088898481
15	24.719467310174206	27.933045860051593	22.800315187367012	24.547171642407186
16	23.91313951820946	26.658137584500196	24.397435916006042	25.0312869812843
17	23.197123915276013	26.738672591880157	26.226493120485294	23.83771037235853
18	23.212622558782588	25.72644459669488	26.740990146049366	24.319942698473167
19	24.22615417818805	25.29117895429059	27.150762692592373	23.33190417492899
20	24.154309998942615	25.77196280904947	26.724224090106507	23.34950310190141
21	24.467940259247403	25.494652967990234	26.24018117479718	23.797225597965188
22	24.275040086444772	25.787895993962774	26.393139749964874	23.543924169627584
23	24.092894813458614	25.868105095287685	26.11887169875272	23.920128392500974
24	24.08290036110391	25.75726082478856	26.258033584256857	23.901805229850677
25	24.15010943201093	25.396591457205638	26.617507962971278	23.83579114781216
26	24.184727897413467	25.819508881302117	26.66063619758888	23.33512702369554
27	24.23299820534399	25.832436488152226	25.974241833880612	23.96032347262317
28	23.69471003776165	25.477850700263478	26.84513523652813	23.982304025446744
29	23.748049995437313	25.519059710334695	27.124690208188788	23.6082000860392
30	23.569344841921076	25.752444657530678	26.669544296426768	24.008666204121482
31	23.663893809667965	25.6973665342281	26.798747941360084	23.839991714743842
32	23.322597746468265	25.591193583851286	27.03912176284758	24.047086906832874
33	23.648214107241923	25.75186526898837	26.76488992341932	23.835030700350384
34	23.955652152500857	25.219334775045155	26.939829051410598	23.885184021043393
35	23.777779870014182	25.173925198042248	27.24896905051254	23.79932588143103
36	23.92842089101267	25.25297552228256	26.606137462828606	24.212466123876165
37	23.102683582880807	24.488001587524604	27.721062077136892	24.688252752457693
38	23.375973915927826	24.58168147245804	28.089010013282483	23.95333459833165
39	22.52688000718442	24.800509282528683	28.47071842730774	24.201892282979156
40	22.697908262515153	23.973468350176642	29.166491643044512	24.16213174426369
41	22.00126596396493	24.133343376068066	29.19850286000669	24.666887799960314
42	21.593666124455556	24.691439389440355	28.818315340904977	24.896579145199112
43	21.74669712319104	23.789150370156857	29.624425862166365	24.83972664448574
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2412.0
1	1847.0
2	1282.0
3	2447.0
4	3612.0
5	3612.0
6	3930.0
7	4248.0
8	3636.5
9	3025.0
10	4193.5
11	5362.0
12	5362.0
13	8718.5
14	12075.0
15	15401.5
16	18728.0
17	19343.5
18	19959.0
19	19959.0
20	24451.0
21	28943.0
22	29368.5
23	29794.0
24	35642.0
25	41490.0
26	41490.0
27	48080.0
28	54670.0
29	62704.5
30	70739.0
31	80820.0
32	90901.0
33	90901.0
34	101286.0
35	111671.0
36	121530.0
37	131389.0
38	137711.5
39	144034.0
40	144034.0
41	153927.5
42	163821.0
43	168175.0
44	172529.0
45	173756.0
46	174983.0
47	174983.0
48	173066.5
49	171150.0
50	177892.5
51	184635.0
52	187589.5
53	190544.0
54	190544.0
55	188389.5
56	186235.0
57	173941.0
58	161647.0
59	148661.5
60	135676.0
61	135676.0
62	128639.5
63	121603.0
64	105756.0
65	89909.0
66	77261.5
67	64614.0
68	64614.0
69	55528.5
70	46443.0
71	40673.0
72	34903.0
73	35010.0
74	35117.0
75	35117.0
76	31758.5
77	28400.0
78	21475.0
79	14550.0
80	10082.0
81	5614.0
82	5614.0
83	4055.0
84	2496.0
85	1825.5
86	1155.0
87	869.0
88	583.0
89	583.0
90	471.0
91	359.0
92	250.5
93	142.0
94	99.5
95	57.0
96	57.0
97	35.5
98	14.0
99	16.5
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2761532.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.80666371583869
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.2471413226957	47.5443284003013
2	10.619648884614028	12.277729437063293
3	3.058736610246565	5.304460758715426
4	1.2765151847395457	2.951643360496025
5	0.6939358856110731	2.0057059189936
6	0.43346997038338725	1.5034471685320214
7	0.2724274356495664	1.102368481169394
8	0.20064135822924484	0.9278726018117665
9	0.14807119784030834	0.7703551745600513
>10	0.8790236026828148	9.884864092660168
>50	0.09775918034286113	3.9108015402946483
>100	0.06540154464370646	6.89156790656293
>500	0.004713797165962844	1.8390456683161611
>1k	0.0022626226396346735	2.1071747336916085
>5k	2.514025155149637E-4	0.9786347568315659
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8197	0.2968279925780328	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7426	0.26890870719586085	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5981	0.21658267946922213	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	5332	0.19308123172210206	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4258	0.15418977582008828	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	2783	0.10077739457663355	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.6211783893867606E-5	0.0
5	1.0863535168160282E-4	0.0	0.0	3.6211783893867606E-5	0.0
6	1.0863535168160282E-4	0.0	0.0	3.6211783893867606E-5	0.0
7	1.0863535168160282E-4	0.0	3.6211783893867606E-5	3.6211783893867606E-5	0.0
8	1.8105891946933804E-4	0.0	3.6211783893867606E-5	3.6211783893867606E-5	0.0
9	1.8105891946933804E-4	0.0	3.6211783893867606E-5	1.0863535168160282E-4	0.0
10	2.5348248725707325E-4	0.0	3.6211783893867606E-5	2.1727070336320564E-4	0.0
11	4.7075319062027886E-4	0.0	3.6211783893867606E-5	2.8969427115094085E-4	0.0
12	4.7075319062027886E-4	0.0	3.6211783893867606E-5	5.069649745141465E-4	0.0
13	5.069649745141465E-4	0.0	3.6211783893867606E-5	5.069649745141465E-4	0.0
14	5.069649745141465E-4	0.0	3.6211783893867606E-5	5.431767584080142E-4	0.0
15	6.156003261957493E-4	0.0	3.6211783893867606E-5	5.431767584080142E-4	3.6211783893867606E-5
16	6.518121100896169E-4	0.0	3.6211783893867606E-5	6.156003261957493E-4	3.6211783893867606E-5
17	8.32871029558955E-4	0.0	3.6211783893867606E-5	6.880238939834845E-4	3.6211783893867606E-5
18	8.32871029558955E-4	0.0	3.6211783893867606E-5	8.690828134528225E-4	3.6211783893867606E-5
19	8.690828134528225E-4	0.0	3.6211783893867606E-5	9.052945973466902E-4	3.6211783893867606E-5
20	8.690828134528225E-4	0.0	3.6211783893867606E-5	0.0010501417329221606	7.242356778773521E-5
21	0.0010501417329221606	0.0	3.6211783893867606E-5	0.0014484713557547043	7.242356778773521E-5
22	0.0011225653007098959	0.0	3.6211783893867606E-5	0.002064071681950454	7.242356778773521E-5
23	0.0011225653007098959	0.0	3.6211783893867606E-5	0.003693601957174496	7.242356778773521E-5
24	0.0011225653007098959	0.0	3.6211783893867606E-5	0.007894168888863138	7.242356778773521E-5
25	0.0011225653007098959	0.0	3.6211783893867606E-5	0.014231231070289969	7.242356778773521E-5
26	0.0011587770846037634	0.0	3.6211783893867606E-5	0.026289755106947883	7.242356778773521E-5
27	0.0013036242201792338	0.0	3.6211783893867606E-5	0.08737903453590254	7.242356778773521E-5
28	0.0015571067074363072	0.0	3.6211783893867606E-5	0.20561050894938027	7.242356778773521E-5
29	0.0015571067074363072	0.0	3.6211783893867606E-5	0.3432152877460772	7.242356778773521E-5
30	0.00166574205911791	0.0	3.6211783893867606E-5	0.49425463836739897	7.242356778773521E-5
31	0.00166574205911791	0.0	3.6211783893867606E-5	0.778155024095321	7.242356778773521E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2040	0.0	23.487745	1
CGAATTA	200	0.0	16.650002	15
GTATCAA	3215	0.0	14.903578	2
GTACTAG	125	2.9622006E-6	14.799999	1
CCGAATT	250	0.0	14.059999	14
GACAGGC	1790	0.0	14.055866	7
CGTGCGA	120	3.3036566E-5	13.874999	10
GTACGTA	120	3.3036566E-5	13.874999	13
TGCATCA	1685	0.0	13.724036	14
CGACGTT	110	2.4583176E-4	13.454545	24
CAGGACA	2115	0.0	13.382979	4
ATTAGAG	430	0.0	13.337209	3
GGACAGG	1985	0.0	13.234257	6
AGACGTA	170	3.7366044E-7	13.058824	5
TACGTAG	85	0.009409099	13.058824	14
GCTGCAT	1795	0.0	12.986073	12
TGCACCG	700	0.0	12.950001	5
GCATCAG	1820	0.0	12.909342	15
ACAGGCT	2025	0.0	12.607408	8
TAACGGC	235	8.094503E-10	12.595745	36
>>END_MODULE