FastQCFastQC Report
Fri 10 Feb 2017
ERR1630069.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630069.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314537
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT43891.39538432680416No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37131.1804652552799828No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28640.9105447053923703No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20190.6418958659871494No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17980.5716338618350146No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16390.5210833701599493No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10980.3490845274164884No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9610.3055284433945768No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6610.21015015721520838No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6260.19902269049428206No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3790.12049456820660209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGTT401.5951791E-632.37532
GGTATCA9400.029.9148941
CGACCAA405.9266255E-527.75000212
TCGTTGT358.855016E-426.42857234
AACCACG451.3202282E-424.6666687
ACGACCA451.3202282E-424.66666811
GATTACT450.00381972320.5555572
CGAACTC555.131686E-420.18181820
TAAGCTA555.131686E-420.18181833
ATTACTC609.216494E-418.53
CTACACA609.216494E-418.54
CCCTACG500.007023803418.49999825
TCAGGAT500.007023803418.4999983
TACGCAT500.007023803418.49999828
TACTGGT500.007023803418.4999987
GTATCAA16250.017.3046152
ACGGGTG752.0612564E-417.2666665
CAACCAC650.001576486517.0769236
ACGTAGG650.001576486517.07692327
TATACCA650.001576486517.0769235