##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630069.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	314537
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.74238324902952	34.0	31.0	34.0	31.0	34.0
2	32.940312268508954	34.0	31.0	34.0	31.0	34.0
3	33.051469302498596	34.0	33.0	34.0	31.0	34.0
4	36.3384562070599	37.0	37.0	37.0	35.0	37.0
5	36.33419597694389	37.0	37.0	37.0	35.0	37.0
6	36.432941116625386	37.0	37.0	37.0	35.0	37.0
7	36.34549830385615	37.0	37.0	37.0	35.0	37.0
8	36.376464454102376	37.0	37.0	37.0	35.0	37.0
9	38.13417817299714	39.0	39.0	39.0	37.0	39.0
10	38.122942610885204	39.0	39.0	39.0	37.0	39.0
11	38.17481568146196	39.0	39.0	39.0	37.0	39.0
12	38.10617192889867	39.0	39.0	39.0	37.0	39.0
13	38.12456722102646	39.0	39.0	39.0	37.0	39.0
14	39.5017025024083	41.0	39.0	41.0	37.0	41.0
15	39.484938178974176	41.0	39.0	41.0	37.0	41.0
16	39.373590388412175	41.0	39.0	41.0	36.0	41.0
17	39.345463331817875	41.0	39.0	41.0	37.0	41.0
18	39.28960026960262	41.0	39.0	41.0	36.0	41.0
19	39.31731719956635	41.0	39.0	41.0	36.0	41.0
20	39.51864804458617	41.0	39.0	41.0	37.0	41.0
21	39.49721336440546	41.0	39.0	41.0	36.0	41.0
22	39.4561848049673	41.0	39.0	41.0	36.0	41.0
23	39.36857666983534	41.0	39.0	41.0	35.0	41.0
24	39.32201299052258	41.0	39.0	41.0	35.0	41.0
25	39.283960233613215	41.0	39.0	41.0	35.0	41.0
26	39.10794914429781	41.0	39.0	41.0	35.0	41.0
27	38.965438088364806	41.0	39.0	41.0	35.0	41.0
28	38.91684603083262	40.0	38.0	41.0	35.0	41.0
29	38.7658717416393	40.0	38.0	41.0	35.0	41.0
30	38.62488355900896	40.0	38.0	41.0	35.0	41.0
31	38.40860693654483	40.0	38.0	41.0	35.0	41.0
32	38.24832054734419	40.0	37.0	41.0	35.0	41.0
33	38.110600660653596	40.0	37.0	41.0	35.0	41.0
34	38.004225258077746	40.0	37.0	41.0	34.0	41.0
35	37.829403218063376	40.0	37.0	41.0	34.0	41.0
36	37.73450818186732	40.0	37.0	41.0	34.0	41.0
37	37.63879925096253	40.0	36.0	41.0	33.0	41.0
38	37.47331474516512	40.0	36.0	41.0	33.0	41.0
39	37.37092933422777	40.0	36.0	41.0	33.0	41.0
40	37.232894699192784	40.0	36.0	41.0	33.0	41.0
41	37.05536391585092	40.0	35.0	41.0	32.0	41.0
42	36.87919386272521	40.0	35.0	41.0	31.0	41.0
43	36.534299621348204	40.0	35.0	41.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	2.0
15	3.0
16	3.0
17	5.0
18	8.0
19	22.0
20	38.0
21	69.0
22	140.0
23	223.0
24	390.0
25	595.0
26	913.0
27	1340.0
28	1841.0
29	2510.0
30	3020.0
31	4031.0
32	4883.0
33	6511.0
34	8929.0
35	12640.0
36	21335.0
37	36797.0
38	56866.0
39	151422.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.615736781364355	19.977935823130505	13.42608341784909	21.98024397765605
2	20.026260821461385	23.107615320296183	34.425202758340035	22.440921099902397
3	20.082534010307214	24.50299964710034	31.116847938398344	24.2976184041941
4	13.642274199855661	17.00563049816079	36.46311880637254	32.88897649561101
5	13.159024216546861	37.68459672470965	35.026085961270056	14.130293097473428
6	31.383589212079976	36.72350152764221	15.002368560773455	16.89054069950435
7	26.88332374251678	31.49963279359821	22.782693292045135	18.83435017183988
8	26.28911701961931	33.65931512031971	20.576275605095745	19.475292254965236
9	24.910582856706842	14.347755589962388	22.208516009245336	38.533145544085436
10	17.68440596813729	27.317294944632902	33.79888534576219	21.19941374146762
11	31.708829167951624	23.444300670509353	22.39927258160407	22.447597579934953
12	20.228780715782246	29.036011661585125	30.120144847823944	20.61506277480869
13	30.480992697202552	21.468698436114035	25.522593526357788	22.52771534032562
14	21.12819795445369	23.677977471648802	28.9031179161752	26.2907066577223
15	24.341492415836612	28.569611842168012	26.015699265905123	21.073196476090253
16	21.051895325510195	27.595481612656066	27.92549048283668	23.427132578997064
17	20.011000295672687	27.97127206020277	28.773085519350666	23.244642124773872
18	20.68405306847843	25.865319501362322	30.064189586598715	23.386437843560532
19	22.18181008911511	25.518778394910612	31.779727027344958	20.51968448862932
20	22.866944111503575	26.243017514632616	31.274540038214898	19.61549833564891
21	21.636246292169126	26.81528723170883	30.610389238785896	20.93807723733615
22	20.91963743534147	26.8346808165653	31.78195252068914	20.46372922740409
23	21.07796539039922	27.109688208382483	30.896842024944597	20.915504376273695
24	20.627461952012005	27.479755958758428	31.085055176338557	20.807726912891013
25	21.05380289123378	27.41458079653586	30.824990382689478	20.70662592954088
26	20.461821661680503	27.576405955420192	30.897795807806393	21.063976575092912
27	20.00432381564013	27.498831615994302	31.239885927569727	21.256958640795837
28	20.12354667336434	27.22604971752131	31.575935422541708	21.074468186572645
29	20.05010539300623	27.39010036974982	32.154563692029875	20.405230545214074
30	20.528268534385465	28.21448668996016	30.722935616477553	20.53430915917682
31	20.976228551807896	28.438943590102276	30.636459303674922	19.948368554414902
32	19.787815106012964	27.963641797308426	31.24783411808468	21.00070897859393
33	19.62853336809342	27.71661203610386	31.49200253070386	21.162852065098857
34	20.276787786492527	26.236023106979466	32.28809329268098	21.19909581384702
35	19.787815106012964	27.048646105227686	31.676082623030037	21.48745616572931
36	20.415722156693807	27.913727160874558	30.712444004997824	20.958106677433815
37	20.059961149244764	26.824507132706167	31.480875063982932	21.634656654066134
38	19.87143007023021	27.3026702740854	31.913256627996073	20.912643027688315
39	19.212366112730773	26.74121009610952	32.58344805221643	21.46297573894327
40	18.948804115255122	26.231254192670495	33.29051908042615	21.529422611648233
41	18.82671990894553	26.0493995936885	32.97481695317244	22.149063544193528
42	18.186731608681967	26.48178115770164	32.98276514368739	22.34872208992901
43	18.22901598222149	25.82716818689057	33.31436365197099	22.62945217891695
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3652.0
1	2334.0
2	1016.0
3	1404.0
4	1792.0
5	1792.0
6	1896.0
7	2000.0
8	1658.5
9	1317.0
10	1725.5
11	2134.0
12	2134.0
13	3701.0
14	5268.0
15	6455.5
16	7643.0
17	6968.0
18	6293.0
19	6293.0
20	7105.5
21	7918.0
22	5935.0
23	3952.0
24	4131.5
25	4311.0
26	4311.0
27	4752.5
28	5194.0
29	6137.5
30	7081.0
31	8204.5
32	9328.0
33	9328.0
34	10715.0
35	12102.0
36	13492.0
37	14882.0
38	15932.0
39	16982.0
40	16982.0
41	18322.5
42	19663.0
43	20169.0
44	20675.0
45	20511.0
46	20347.0
47	20347.0
48	20328.0
49	20309.0
50	20391.5
51	20474.0
52	20308.0
53	20142.0
54	20142.0
55	20435.0
56	20728.0
57	19130.5
58	17533.0
59	16050.0
60	14567.0
61	14567.0
62	12528.0
63	10489.0
64	8684.5
65	6880.0
66	5600.5
67	4321.0
68	4321.0
69	3396.0
70	2471.0
71	1938.0
72	1405.0
73	1105.0
74	805.0
75	805.0
76	614.5
77	424.0
78	320.5
79	217.0
80	168.0
81	119.0
82	119.0
83	82.0
84	45.0
85	34.5
86	24.0
87	22.0
88	20.0
89	20.0
90	16.0
91	12.0
92	7.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	314537.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.97752995805354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.95658662198355	52.87378449161885
2	9.90471950527364	12.475495387389758
3	2.5282747591152783	4.776734981531191
4	1.0420305680702853	2.6249804527141576
5	0.5528837854619512	1.7409627581126037
6	0.3684689285007846	1.3923157789962028
7	0.26103613168083173	1.1507587562144792
8	0.17339496894945766	0.8735989481272185
9	0.16309400489001963	0.9244131821046127
>10	0.9794751459196669	11.58845492144189
>50	0.053795445625966484	2.1784863802383034
>100	0.011165092488408078	1.0818920770402913
>500	0.0015225126120556472	0.7184914000551181
>1k	0.0035525294281298437	5.599630484415333
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4389	1.39538432680416	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3713	1.1804652552799828	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2864	0.9105447053923703	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2019	0.6418958659871494	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1798	0.5716338618350146	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1639	0.5210833701599493	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1098	0.3490845274164884	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	961	0.3055284433945768	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	661	0.21015015721520838	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	626	0.19902269049428206	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	379	0.12049456820660209	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.179276205978947E-4	0.0
2	0.0	0.0	0.0	3.179276205978947E-4	0.0
3	0.0	0.0	0.0	3.179276205978947E-4	0.0
4	0.0	0.0	0.0	3.179276205978947E-4	0.0
5	0.0	0.0	0.0	3.179276205978947E-4	0.0
6	0.0	0.0	0.0	3.179276205978947E-4	0.0
7	0.0	0.0	0.0	6.358552411957894E-4	0.0
8	0.0	0.0	0.0	6.358552411957894E-4	0.0
9	0.0	0.0	0.0	6.358552411957894E-4	0.0
10	0.0	0.0	0.0	6.358552411957894E-4	0.0
11	0.0	0.0	0.0	6.358552411957894E-4	0.0
12	0.0	0.0	0.0	9.537828617936841E-4	0.0
13	0.0	0.0	0.0	9.537828617936841E-4	0.0
14	0.0	0.0	0.0	9.537828617936841E-4	0.0
15	0.0	0.0	0.0	9.537828617936841E-4	0.0
16	0.0	0.0	0.0	9.537828617936841E-4	0.0
17	0.0	0.0	0.0	0.0012717104823915788	0.0
18	0.0	0.0	0.0	0.0012717104823915788	0.0
19	0.0	0.0	0.0	0.0015896381029894735	0.0
20	0.0	0.0	0.0	0.0025434209647831577	0.0
21	0.0	0.0	0.0	0.0028613485853810523	0.0
22	0.0	0.0	0.0	0.005086841929566315	0.0
23	0.0	0.0	0.0	0.006358552411957894	0.0
24	0.0	0.0	0.0	0.011127466720926314	0.0
25	0.0	0.0	0.0	0.021937005821254733	0.0
26	0.0	0.0	0.0	0.04069473543653052	0.0
27	0.0	0.0	0.0	0.13416545589231155	0.0
28	0.0	0.0	0.0	0.30330295005039154	0.0
29	0.0	0.0	0.0	0.5067766272330442	0.0
30	0.0	0.0	0.0	0.7461761255432589	0.0
31	0.0	0.0	0.0	1.1601178875617177	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATCGTT	40	1.5951791E-6	32.375	32
GGTATCA	940	0.0	29.914894	1
CGACCAA	40	5.9266255E-5	27.750002	12
TCGTTGT	35	8.855016E-4	26.428572	34
AACCACG	45	1.3202282E-4	24.666668	7
ACGACCA	45	1.3202282E-4	24.666668	11
GATTACT	45	0.003819723	20.555557	2
CGAACTC	55	5.131686E-4	20.181818	20
TAAGCTA	55	5.131686E-4	20.181818	33
ATTACTC	60	9.216494E-4	18.5	3
CTACACA	60	9.216494E-4	18.5	4
CCCTACG	50	0.0070238034	18.499998	25
TCAGGAT	50	0.0070238034	18.499998	3
TACGCAT	50	0.0070238034	18.499998	28
TACTGGT	50	0.0070238034	18.499998	7
GTATCAA	1625	0.0	17.304615	2
ACGGGTG	75	2.0612564E-4	17.266666	5
CAACCAC	65	0.0015764865	17.076923	6
ACGTAGG	65	0.0015764865	17.076923	27
TATACCA	65	0.0015764865	17.076923	5
>>END_MODULE