FastQCFastQC Report
Fri 10 Feb 2017
ERR1630068.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630068.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2146093
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT65830.30674346358708593No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT55300.2576775563780321No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42440.19775471053677546No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30400.1416527615532039No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT21890.1019993075789353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15700.026.512741
GTATCAA25450.016.2102172
CGCGCTT2151.2732926E-1114.62790712
GTTCGCG1406.001337E-714.53571530
ATCTCGC4750.014.02105211
AATCTCG4750.014.02105210
GTATATC3750.013.8133341
TTCCTCG5450.013.57798219
GGGTAGA3150.013.5079371
CGCGGAA2102.044544E-913.21428533
GTTCTAC9700.013.1597931
GTAAGAC1703.7352947E-713.0588233
TTAGAGT2152.903107E-912.9069774
GCGACTT3450.012.86956522
GTCTTAG2602.3646862E-1112.8076931
CGGTCGA1451.3731751E-512.7586220
GTGCTAG5100.012.6960781
CACACTA9500.012.65789510
ATCAACG32600.012.5981594
TAGCACT5600.012.5535724
Produced by FastQC (version 0.11.5)