FastQCFastQC Report
Fri 10 Feb 2017
ERR1630067.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630067.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456435
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50230.3448832251353476No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT45200.31034684005808705No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35760.24553103983356622No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26340.18085256121969054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11250.029.1066671
TATCCGG400.001931433923.12510
TTATGCG903.8307917E-922.611114
TATGCGG951.6768718E-719.4736845
CGAATTA1103.853711E-818.515
CAGTGCG701.21948135E-418.59
GTATCAA18350.017.6430512
CGTGCGA852.7239237E-517.41176410
TTTACGC700.002592939915.85714233
CTATCCG700.002592939915.8571429
CGACCAA1455.3480107E-815.31034512
GACGTTA855.3653715E-415.23529425
GTATAGG2252.7284841E-1113.97777751
ACACCGT800.006299687513.8756
AGGGCGC950.001245352813.6315795
TACGAGT1102.4570667E-413.4545454
CGAAGGT1254.8461174E-513.324
CCGAATT1551.888302E-613.12903214
GACGGTT850.00940630513.05882411
TATCAAC25250.012.9683163
Produced by FastQC (version 0.11.5)