##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630067.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1456435
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.71078489599604	34.0	31.0	34.0	31.0	34.0
2	32.951902419263476	34.0	31.0	34.0	31.0	34.0
3	33.08642953513202	34.0	33.0	34.0	31.0	34.0
4	36.42747599446594	37.0	37.0	37.0	35.0	37.0
5	36.361480601605976	37.0	37.0	37.0	35.0	37.0
6	36.41686858665165	37.0	37.0	37.0	35.0	37.0
7	36.35027721800149	37.0	37.0	37.0	35.0	37.0
8	36.357205093258536	37.0	37.0	37.0	35.0	37.0
9	38.098756209511585	39.0	38.0	39.0	37.0	39.0
10	38.060475064112026	39.0	38.0	39.0	37.0	39.0
11	38.11045395091439	39.0	38.0	39.0	37.0	39.0
12	38.0402812346586	39.0	38.0	39.0	35.0	39.0
13	38.06215931366659	39.0	38.0	39.0	37.0	39.0
14	39.478900877828394	41.0	39.0	41.0	37.0	41.0
15	39.475455478617306	41.0	39.0	41.0	37.0	41.0
16	39.39194128127929	41.0	39.0	41.0	36.0	41.0
17	39.323972576874354	41.0	39.0	41.0	36.0	41.0
18	39.221805298554344	40.0	39.0	41.0	36.0	41.0
19	39.25364194076632	40.0	39.0	41.0	36.0	41.0
20	39.46239413362079	41.0	39.0	41.0	37.0	41.0
21	39.4640557251096	41.0	39.0	41.0	37.0	41.0
22	39.47079958940839	41.0	39.0	41.0	37.0	41.0
23	39.40495593692818	41.0	39.0	41.0	36.0	41.0
24	39.36652373775692	41.0	39.0	41.0	36.0	41.0
25	39.33578017556568	41.0	39.0	41.0	36.0	41.0
26	39.19407594571677	41.0	39.0	41.0	36.0	41.0
27	39.1066357235304	40.0	39.0	41.0	36.0	41.0
28	39.06528338030877	40.0	39.0	41.0	35.0	41.0
29	38.95838125285371	40.0	39.0	41.0	35.0	41.0
30	38.871018617377366	40.0	39.0	41.0	35.0	41.0
31	38.74142752680346	40.0	38.0	41.0	35.0	41.0
32	38.65712647663644	40.0	38.0	41.0	35.0	41.0
33	38.57981646966737	40.0	38.0	41.0	35.0	41.0
34	38.51033860076145	40.0	38.0	41.0	35.0	41.0
35	38.37491408816734	40.0	38.0	41.0	35.0	41.0
36	38.36496994373247	40.0	38.0	41.0	35.0	41.0
37	38.32060339115718	40.0	38.0	41.0	34.0	41.0
38	38.2201780374682	40.0	38.0	41.0	34.0	41.0
39	38.17829837926169	40.0	38.0	41.0	34.0	41.0
40	38.11598526539118	40.0	38.0	41.0	34.0	41.0
41	38.009424382138576	40.0	38.0	41.0	34.0	41.0
42	37.915381736912394	40.0	38.0	41.0	33.0	41.0
43	37.6164113056882	40.0	38.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	0.0
12	1.0
13	2.0
14	3.0
15	11.0
16	7.0
17	23.0
18	54.0
19	90.0
20	163.0
21	290.0
22	535.0
23	921.0
24	1550.0
25	2373.0
26	3460.0
27	4778.0
28	6637.0
29	9204.0
30	11985.0
31	15330.0
32	19758.0
33	25547.0
34	35506.0
35	49706.0
36	75519.0
37	133989.0
38	307899.0
39	751092.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.4511907500163	20.104707728116942	12.673892072080115	24.770209449786638
2	18.335868061396493	21.968848592625143	35.84409877543454	23.85118457054383
3	18.9527167364146	24.005534060909	31.492926220531643	25.54882298214476
4	14.141585446655705	16.430874017721354	36.436778846979095	32.99076168864384
5	13.559341817520176	37.24251339743964	35.60866087398339	13.589483911056794
6	32.76418103107932	36.750352744887344	15.883990703326958	14.601475520706384
7	27.15033626629407	31.435251144060665	22.944243993037794	18.47016859660747
8	25.383007137290715	35.033146003769474	20.99393381784975	18.58991304109006
9	25.247127403557318	15.090683758629805	21.11532612166008	38.546862716152795
10	15.774202075616145	27.71438478201911	35.38455200541047	21.126861136954275
11	33.40149062608355	22.959898656651344	23.03679875861264	20.601811958652462
12	19.804591348051922	26.345974931939974	31.79249331415408	22.05694040585402
13	29.033290191460654	21.377473076381712	25.90853694122978	23.680699790927846
14	21.09939681482524	22.39557549770501	27.212405634305686	29.292622053164063
15	23.477532467978317	29.335809699711966	24.128024937604494	23.058632894705223
16	22.746638195319395	28.26202336527205	25.855393477910106	23.135944961498453
17	21.89016330972546	27.992529704380903	27.433012801807156	22.684294184086482
18	21.751605804584482	27.209796523703428	28.352792949908512	22.68580472180358
19	22.87984015764521	26.77249585460388	28.84543422809806	21.502229759652852
20	23.043115552702318	26.727454366312266	28.553419823061105	21.676010257924318
21	22.347100969147267	27.244744873612625	27.898052436257025	22.510101720983087
22	22.52932674647341	26.945864388043404	28.06654605251865	22.458262812964534
23	22.46245112208921	27.108041210215355	27.92606604482864	22.503441622866795
24	22.761331607658423	27.152945376896326	27.90038690363799	22.185336111807256
25	22.816809538359077	26.731574014631615	28.058238095074618	22.39337835193469
26	22.451328071626953	27.069247855208094	27.96506538225187	22.514358690913085
27	22.379234226038236	27.17766326681246	27.658357564875878	22.784744942273427
28	22.199205594482418	27.181782915131812	28.043544682735583	22.575466807650187
29	22.25131914572226	27.348971976092308	28.203455698331886	22.19625317985355
30	22.250838520085004	27.636111463951362	27.933138107776866	22.17991190818677
31	22.593936564281965	27.04446130448664	28.162327875943653	22.19927425528774
32	21.89709805106304	27.13014998952923	28.348673301589155	22.62407865781858
33	21.98395396979611	27.039517726503416	28.466563904328034	22.50996439937244
34	22.237655645463068	26.361080309110946	28.47906017089674	22.922203874529245
35	22.050554950959018	26.607915904245637	28.697332871017245	22.644196273778093
36	22.171466629132095	26.51845087491031	28.677009272641758	22.633073223315836
37	22.261412284104683	25.801494745731873	28.839392077229675	23.097700892933773
38	21.868809799270135	26.125573746854474	29.39176825604988	22.61384819782551
39	21.61929643272786	25.98674159849221	29.652541994665054	22.741419974114876
40	21.249283352844444	25.57436480172476	30.20546745992784	22.970884385502956
41	20.951570100965714	25.660808755625897	30.232451156419614	23.155169986988778
42	20.864302217400706	25.58047561339847	30.117924933141545	23.437297236059283
43	20.563293246866493	25.56509559300621	30.261220033849778	23.61039112627752
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1815.0
1	1366.0
2	917.0
3	1616.0
4	2315.0
5	2315.0
6	2533.5
7	2752.0
8	2431.0
9	2110.0
10	2966.5
11	3823.0
12	3823.0
13	5940.0
14	8057.0
15	10601.0
16	13145.0
17	13798.0
18	14451.0
19	14451.0
20	17310.0
21	20169.0
22	21424.5
23	22680.0
24	26226.5
25	29773.0
26	29773.0
27	34145.0
28	38517.0
29	45137.5
30	51758.0
31	56446.5
32	61135.0
33	61135.0
34	65218.0
35	69301.0
36	74424.5
37	79548.0
38	82743.0
39	85938.0
40	85938.0
41	90167.0
42	94396.0
43	92598.5
44	90801.0
45	91790.5
46	92780.0
47	92780.0
48	92340.5
49	91901.0
50	93688.5
51	95476.0
52	96596.0
53	97716.0
54	97716.0
55	91769.0
56	85822.0
57	79996.0
58	74170.0
59	67293.0
60	60416.0
61	60416.0
62	55223.5
63	50031.0
64	43984.0
65	37937.0
66	32669.5
67	27402.0
68	27402.0
69	23022.5
70	18643.0
71	15824.0
72	13005.0
73	10411.0
74	7817.0
75	7817.0
76	6152.5
77	4488.0
78	3627.5
79	2767.0
80	2057.5
81	1348.0
82	1348.0
83	1020.0
84	692.0
85	510.0
86	328.0
87	243.5
88	159.0
89	159.0
90	114.5
91	70.0
92	50.0
93	30.0
94	25.5
95	21.0
96	21.0
97	15.0
98	9.0
99	7.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1456435.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.94994340816824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.74134269425848	59.80810669917649
2	8.481386457777491	11.69582232573125
3	2.108275608967833	4.360964517814605
4	0.8334191458705549	2.2985681177223465
5	0.4543038921322516	1.5662113826314654
6	0.27705523846058344	1.146176580767627
7	0.18708367335088702	0.9029586083095124
8	0.13766755786896634	0.7593736259364764
9	0.10794009156051423	0.6698216884112959
>10	0.5745822039317546	7.49011441027351
>50	0.058138693362432076	2.7906334099035353
>100	0.03679938682884238	4.59788003650115
>500	0.0013034811591340707	0.5746466171691575
>1k	6.016066888311095E-4	0.9914589914368249
>5k	1.0026778147185159E-4	0.3472629882147111
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5023	0.3448832251353476	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4520	0.31034684005808705	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3576	0.24553103983356622	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2634	0.18085256121969054	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	6.866080532258563E-5	0.0	0.0	0.0	0.0
8	6.866080532258563E-5	0.0	0.0	0.0	0.0
9	6.866080532258563E-5	0.0	0.0	6.866080532258563E-5	0.0
10	6.866080532258563E-5	0.0	0.0	1.3732161064517126E-4	0.0
11	6.866080532258563E-5	0.0	0.0	2.0598241596775688E-4	0.0
12	6.866080532258563E-5	0.0	0.0	2.7464322129034253E-4	0.0
13	6.866080532258563E-5	0.0	0.0	3.433040266129281E-4	0.0
14	6.866080532258563E-5	6.866080532258563E-5	0.0	3.433040266129281E-4	0.0
15	6.866080532258563E-5	6.866080532258563E-5	0.0	4.1196483193551377E-4	0.0
16	6.866080532258563E-5	6.866080532258563E-5	0.0	4.1196483193551377E-4	0.0
17	6.866080532258563E-5	6.866080532258563E-5	0.0	4.806256372580994E-4	0.0
18	6.866080532258563E-5	6.866080532258563E-5	0.0	5.492864425806851E-4	0.0
19	1.3732161064517126E-4	6.866080532258563E-5	0.0	6.179472479032707E-4	0.0
20	1.3732161064517126E-4	6.866080532258563E-5	0.0	9.612512745161988E-4	6.866080532258563E-5
21	2.0598241596775688E-4	6.866080532258563E-5	0.0	0.0010985728851613701	6.866080532258563E-5
22	2.0598241596775688E-4	6.866080532258563E-5	0.0	0.0017165201330646407	6.866080532258563E-5
23	2.0598241596775688E-4	6.866080532258563E-5	0.0	0.003158397044838939	6.866080532258563E-5
24	2.0598241596775688E-4	6.866080532258563E-5	0.0	0.00707206294822632	6.866080532258563E-5
25	2.0598241596775688E-4	6.866080532258563E-5	0.0	0.013457517843226784	6.866080532258563E-5
26	2.0598241596775688E-4	6.866080532258563E-5	0.0	0.0234133346150017	6.866080532258563E-5
27	2.0598241596775688E-4	6.866080532258563E-5	0.0	0.08088242867000588	1.3732161064517126E-4
28	2.0598241596775688E-4	1.3732161064517126E-4	0.0	0.18284372457404552	1.3732161064517126E-4
29	2.0598241596775688E-4	2.7464322129034253E-4	0.0	0.3034807595258285	1.3732161064517126E-4
30	2.0598241596775688E-4	2.7464322129034253E-4	0.0	0.43599611379841874	1.3732161064517126E-4
31	2.0598241596775688E-4	2.7464322129034253E-4	0.0	0.6990356589892442	1.3732161064517126E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1125	0.0	29.106667	1
TATCCGG	40	0.0019314339	23.125	10
TTATGCG	90	3.8307917E-9	22.61111	4
TATGCGG	95	1.6768718E-7	19.473684	5
CGAATTA	110	3.853711E-8	18.5	15
CAGTGCG	70	1.21948135E-4	18.5	9
GTATCAA	1835	0.0	17.643051	2
CGTGCGA	85	2.7239237E-5	17.411764	10
TTTACGC	70	0.0025929399	15.857142	33
CTATCCG	70	0.0025929399	15.857142	9
CGACCAA	145	5.3480107E-8	15.310345	12
GACGTTA	85	5.3653715E-4	15.235294	25
GTATAGG	225	2.7284841E-11	13.9777775	1
ACACCGT	80	0.0062996875	13.875	6
AGGGCGC	95	0.0012453528	13.631579	5
TACGAGT	110	2.4570667E-4	13.454545	4
CGAAGGT	125	4.8461174E-5	13.32	4
CCGAATT	155	1.888302E-6	13.129032	14
GACGGTT	85	0.009406305	13.058824	11
TATCAAC	2525	0.0	12.968316	3
>>END_MODULE